Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Escherichia coli K12"”.
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Resistance to colicins in Escherichia coli K12 / John K. Davies
vii, 185 leaves : ill., tables ; 29 cm.
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The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12
… the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by …
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… of a major cell envelope protein from E. coli K12, the so-called "succinate eluted protein" (SEP) is reported. SEP was released by EDTA-sucrose and osmotic shock treatments, or during the formation of spheroplasts. The protein was purified on columns of aspartate-Sepharose (A-S) an …
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A Genetic and Biochemical Analysis of Attenuation Regulation in the Threonine Operon of Escherichia Coli-K12
Threonine biosynthesis in Escherichia coli K-12 was proposed to be regulated by an attenuation mechanism. A genetic and biochemical analysis was performed to provide more evidence that attenuation was the mechanism utilized to regulate threonine biosynthesis.
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The Deoxythymidine Kinase-Deoxythymidylate Kinase Enzymes in SALMONELLA POTSDAM and ESCHERICHIA COLI K12 Infected with Bacteriophage P3
… It is also capable of infecting <em>Escherichia coli</em> K12, but undergoes a host-controlled modification of its nucleic acid in the <em>E. coli</em>. The resultant P3 is found to be restricted in its growth in the <em>Salmonella</em> host as a result of this restriction.</p> <p>The …
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Proteome, transcriptome and metabolome plasticity in closely related strains of Escherichia coli - K12 and its diversity during molecular evolution processes
Escherichia coli, originally known as Bacterium coli commune, was identified in 1885 by the German pediatrician, Theodor Escherich. They are Gram-negative, straight and rod-shaped bacteria from the Enterobacteriaceae family. Due to its rapid growth rate, simple nutritional requirements and …
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Polysulfone ultrafiltration membranes impregnated with silver nanoparticles show improved biofouling resistance and virus removal
… towards a variety of bacteria, including Escherichia coli K12 and Pseudomonas mendocina KR1, and enhanced the removal of MS2 bacteriophage. Nanosilver incorporation also increased membrane hydrophilicity, reducing the potential for other types of membrane fouling. Thus, the incorporation …
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Fingerprinting and characterisation of Escherichia coli isolates using DNA arrays
Two commercially available DNA whole genome Escherichia coli K12 arrays were compared to identify a subset of markers for typing. The arrays were identical in probe composition but different in substrate (membrane and glass slide arrays) and probe preparation (radio- and fluorescent-labelled). …
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CoryneRegNet : a reference database and analysis platform for corynebacterial gene regulatory networks
… 2 mycobacteria and the model organism Escherichia coli K12. As the previous versions, CoryneRegNet provides a web-based user interface to access the database content, to allow various queries, and to support the reconstruction, analysis and visualization of regulatory networks at …
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Studies on the mode of action of diacetyl and carbon dioxide as inhibitors of meat spoilage bacteria
… of CO2 on pure cultures of Pseudomonas fragi, Escherichia coli O157:H7 (both associated with meat), and Escherichia coli K12 W3110 (reference organism) in AOAC synthetic broth was studied using two-dimensional electrophoresis (2-DE) to determine the effect of CO2 on cellular proteins. Pure …
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Controlling Pseudomonas aeruginosa Persister Cells by Human Granulocyte Macrophage Colony-Stimulating Factor
… cells of P. aeruginosa and the non-pathogenic Escherichia coli K12 persister cells</p> <p>were not affected by GM-CSF.</p> <p>DNA microarray and qPCR analyses revealed that GM-CSF induced flagella and pyocin associated genes in persister cells of P. aeruginosa PAO1, while the same genes in …
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The role of signal regulatory protein alpha in regulating macrophage subset activity in homeostasis and inflammation
… with either Staphylococcus aureus LTA, Escherichia coli K12 LPS or Porphyromonas gingivalis LPS. TNFα production was measured by ELISA. M2-like Mφs were found to express higher levels of SIRPα than M1-like Mφs, and K562 cells expressed greater CD47 than HL-60 cells. In Mφ monoculture, …
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Avaliação da técnica de ATP-bioluminescência no controle do procedimento de higienização na indústria de laticínios
… de uso em um laticínio, ii) a adesão de Escherichia coli K12 e de esporos de Bacillus sporothermodurans, em suspensões, contendo cerca de 10 4 e 10 6 UFC/mL, em aço inoxidável e em polietileno de baixa densidade a 37°C e tempo de adesão de 24h. e, iii) o procedimento de higienização em …
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Physiological roles and regulation of the cryptic prophage-encoded small protein DicB and small RNA DicF in Escherichia coli
… DicB encoded on the Qin cryptic prophage of Escherichia coli K12. Bacterial genomes harbor cryptic prophages that have lost genes required for induction, excision from host chromosomes, or production of phage progeny. DicB and DicF are encoded on the dicBF operon, which is located in the …
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The Functional Characterization of the LysR-Type Transcriptional Regulator QseD and the SorC-Type Transcriptional Regulator LsrR in Enterohemorrhagic Escherichia coli
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a human pathogen responsible for numerous outbreaks of hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) throughout the world. EHEC is able to sense and respond to biotic cues from its environment, such as the human host produced …
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Isolation and biological characterisation of plant-derived chemicals for antibacterial purposes in food animals and humans
… epidemic. Pathogens such Listeria monocytogenes, Escherichia coli and Staphylococcus aureus have been the source of a variety of human and animal diseases resulting in economic losses. The ease with which these pathogens adapt to antibacterial remedies means that many antibacterial remedies are …
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Cargo delivery into gram-negative bacteria via enterobactin uptake machinery
… Cargo Delivery to the Cytoplasm of Escherichia coli and Pseudomonas aeruginosa: Syntheses of Monofunctionalized Enterobactin Scaffolds and Evaluation of Enterobactin-cargo Conjugate Uptake This Chapter describes the design and syntheses of monofunctionalized enterobactin (Ent, L- and …
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Incorporation of selected plant extracts into edible chitosan films and the effect on the antiviral, antibacterial and mechanical properties of the material
… also investigated against Listeria innocua and Escherichia coli K12. The viral infectivity after treatments was measured by plaque assays. The 1, 1.5 and 2.5% aqueous GTE solutions significantly (p<0.05) reduced murine norovirus (MNV-1) plaques by 1.69, 2.47, and 3.26 log after 3 h exposure, …