Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 3 of 3 for “"Error prone polymerase chain reaction"”.
-
Identification of Residues Critical to Epithelial Sodium Channel Assembly
… that express ENaC and show growth inhibition. Error prone polymerase chain reaction (EP-PCR) was employed to promote random mutagenesis in the extracellular loop of alpha-ENaC. The levels of growth inhibition were monitored in the yeast strain S1InsE4A and subsequently characterized. The …
-
Uncovering the Critical Amino Acids in the Beta Bubunit of the Epithelial Sodium Channel (ENaC)
… is not yet fully understood. In this study, error prone polymerase chain reaction (EP-PCR) was used to introduce random mutations in the extracellular loop of the beta subunit of ENaC in order to identify residues that may be critical to proper function. A yeast screen, dilution survival …
-
Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
Mycolic acids are long chain lipids from the cell walls of Mycobacterium tuberculosis. The Nkuku phage display library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic …