Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"Erk signalling"”.
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Investigating the role of Neuronatin in neural development
… understood. For instance, in Xenopus, calcium signalling is known to play a pivotal role in neural induction but whether a similar mechanism occurs in mammals is unknown. As part of a wider study on genes regulating neural stem cell (NSC) development, we have discovered a novel role for …
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The role of mechanotransduction in signalling and pluripotent stem cell fate
… mechanisms of interplay between soluble factor signalling and mechanical cues remain unclear. Further understanding of how mechanical factors of the extracellular matrix (ECM) affect pluripotency and cell fate will not only improve the differentiation of pluripotent stem cells in vitro, but also …
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Regulation of polycomb repressive complexes at the neural differentiation gene Pax6
… due to loss of fibroblast growth factor (FGF) signalling. This highly coordinated onset of gene expression implies the involvement of a higher order mechanism to co-ordinately regulate hundreds of genes at the same time. One possible mechanism underlying this phenomenon is the regulation of …
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The crosstalk between the ERK and the cAMP signalling pathways in PC12 Cells
The extracellular-regulated kinase (ERK) signalling pathway is involved in the control of different biological processes such as survival, proliferation and differentiation. In PC12 cells, the ERK signalling pathway integrates different external stimuli: epidermal growth factor (EGF) stimulates the …
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B cell signalling in mechanisms of central and peripheral tolerance
… help. On the other hand, mitogenic BCR-mediated signalling of mature B cells can be suppressed by co-ligation of the inhibitory receptor FcγRIIb. This allows the maintenance of B cell homeostasis in the periphery, as cross-linking of the BCR and FcγRIIb by immune complexes enables the system to …
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ENHANCING CARDIAC TRANSDIFFERENTIATION OF FIBROBLASTS USING SINGLE-CELL TRANSCRIPTOMICS ANALYSIS AND HIGH-THROUGHPUT CHEMICAL LIBRARY SCREENING APPOACHES
… could function co-linearly with TGF-β or MEK/ERK signalling pathways, although this remains to be proven empirically. Next, single-cell transcriptomic studies revealed trajectory of cardiac transdifferentiation which includes an unstable intermediate subpopulation that ultimately progresses …
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Systems biology approaches to the computational modelling of trypanothione metabolism in Trypanosoma brucei
… of information content is illustrated using the ERK signalling pathway. We propose a novel multi-objective optimization algorithm (MoPSwarm) for the validation of perturbation-based models of biological systems, and perform a comparative study to determine the factors crucial to the performance …
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The role of sphingosine kinases in pulmonary fibrosis
… block extracellular regulated kinase (P-ERK) signalling, suggesting that ERK 1 and 2 are involved in SK2 mediated signalling at high concentrations of TGF-β1, although the functional consequence requires further study.
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The Role of p66Shc in Stem Cells: Signalling, Identity and Apoptosis
… receptor tyrosine kinases (RTKs) to downstream signalling, thereby shaping cell fate decisions. While the canonical isoforms p52Shc and p46Shc act as Ras–MAPK transducers, the longer p66Shc isoform harbours an additional CH2 domain that confers distinct regulatory properties. Despite …
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PKB/Akt : a critical regulator of lymphocyte, development and function
… and calcineurin activity. In addition, when TCR signalling was inhibited by the MEKinase inhibitor PD98059 or the Srckinase inhibitor PP1 myr PKB tg thymocytes again were more resistant to inhibition. Western blot analysis revealed myr PKB enhances activation of the kinases Lck, Raf and Erk after …
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Probabilistic reasoning and inference for systems biology
… concentrations, with an example portion of the ERK signalling pathway. We demonstrate that the resulting model approximates the same dynamic system as traditionally defined using ordinary differential equations. The advantage of the new approach is quantitative logical analysis; we formulate a …
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p38 MAPK Signalling in Endothelial Apoptosis
… in the treatment of cancer. We investigated signalling pathways in endothelial apoptosis induced by the inflammatory cytokine tumour necrosis factor alpha (TNF), the main target of which is the endothelium, and by the anticancer drug doxorubicin. Doxorubicin (also called Adriamycin) is a …
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Control of DAPK-1 degradation
… role of DAPK-1 as a survival factor in the TNF-α signalling pathway. In addition, a splice variant of DAPK-1, which I have called s-DAPK-1, was discovered. s-DAPK-1 shares part of DAPK-1’s ankyrin repeats region and cytoskeletal binding domain, and possesses an unique tail region, which contains a …
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Elucidating oncogenic mechanisms in human B cell malignancies
… of the MAPK pathway. I identified Erk negative feedback control via DUSP6 to be crucial for NRASG12D-mediated pre-B cell transformation and investigated its potential as a therapeutic target. I showed that a small molecule inhibitor of DUSP6 (BCI) selectively induced cell death in …
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The role of cellular signalling pathways and translation initiation factors in Herpesvirus infection
… quiescence, specifically the role of host cell signalling and translation initiation during reactivation from a non productive state. To achieve these objectives, a new system was established to study HSV-1 quiescence. The system employs the use of serum starved and temperature elevated primary …
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The effect of cAMP elevation on intracellular signalling pathways in prostate epithelial cells
Chronic IL-6 signalling contributes to the pathophysiology of many diseases including prostate cancer. Relevant to prostate cancer is the ability of the pro-inflammatory cytokine IL-6 to activate the oncogenic signalling protein STAT3, thus inhibition of STAT3 activation is a popular avenue of …
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Estrogenic modulation of excitatory synaptic transmission at hippocampal temporoammonic-CA1 synapses
… LTP and GPER1-mediated LTP utilise distinct signalling mechanisms - ERα-mediated LTP involves PI3-K signalling whereas GPER1-mediated LTP involves ERK signalling and transactivation of EGFR. Further, ERα, GPER1 and hippocampal-synthesised E2 have been implicated in the induction of …
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The Regulation of Oncogenic MAP Kinase Signalling by Dual-Specificity MAP Kinase Phosphatases
Oncogenic activation of the Ras-ERK pathway is frequently observed in human cancers. Dual-specificity MAP kinase phosphatases (DUSPs or MKPs) are important negative regulators of this pathway and could therefore play an important role in modulating the oncogenic potential of signalling. DUSP5 and …
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The role of intracellular and extracellular cysteines in regulating human proteinase-activated receptor-2 (hPAR2) function
… still retained the ability to signal through ERK MAP kinase and internalise post-activation with trypsin but was incapable of agonist mediated Ca2+ mobilisation. Removal of C22 resulted in a receptor with similar ERK signalling to wt-hPAR2 but altered agonist-mediated Ca2+ mobilisation. …
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Reverse genetics analysis of biological functions of zinc transporters Znt1 (Slc30a1) & Zip10 (Slc39a10) in zebrafish
… its effects on extracellular-regulated kinases (ERK1/2). Investigations were also conducted on embryos with znt1 gene knockdown, through deployment of antisense morpholino- modified oligonucleotides (MO) in embryos of wild-type background, and in all of the studies it was shown that zebrafish …
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