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Showing 1 to 20 of 26 for “"Equilibrium dialysis"”.
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Binding study of nickel complex to protein by equilibrium dialysis
… Nickel complex was labeled with Ni63 during the dialysis, and counted by a liquid scintillation counter.</p>
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Equilibrium Dialysis Studies of Purine and Pyrimidine Binding to Rabbit Myofibrils
Made available in DSpace on 2014-12-05T20:55:38Z (GMT). No. of bitstreams: 1 5805447.pdf: 2201465 bytes, checksum: af98da1dc92c66e90077493b47db502e (MD5) Previous issue date: 1958
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Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR
Direct binding of ligands to PyrR was studied by equilibrium dialysis. The dissociation constant calculated for UMP was 27 +/- 3 muM, whereas the KD for PRPP was 18 +/- 2 muM. There was no detectable binding of uracil or PPi to PyrR, which was consistent with each of the four proposed …
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The purification and characterization of phosphoenolpyruvate carboxykinase
… molecular weight, determined by sedimentation equilibrium is 73,300. The ultraviolet and visible absorption spectra of the enzyme revealed no characteristic chromophores. The amino acid composition of the enzyme is reported. Fifteen sulfhydryl groups per molecule of phosphoenolpyruvate …
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Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions
… blood proteins. The results are compared with equilibrium dialysis. The systems studied using this technique includes, the binding of Cu(ll) to HSA and BSA, ternary Cu(ll)-HSA-amino acid systems, as well as competitive studies of the Cu(ll)-HSA system with Mg(ll), Ca(ll) and Zn(ll). The …
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FACTORS AFFECTING THE TRANSPORT OF SOLUTES THROUGH BRONCHIAL MUCUS
… Using the techniques of ultrafiltration and equilibrium dialysis the binding of benzyl-penicillin, cephalexin, chloramphenicol. tetracycline and decamethonium iodide to reconstituted human bronchial mucus and to human serum albumin has been determined. lt was found that only tetracycline was …
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Ligand Binding and Catalysis by Phosphoribosyl Pyrophosphate Synthetase
… from Salmonella typhimurium has been studied by equilibrium dialysis of these compounds labeled with ('32)P. The simplest model that fits the data is the suggestion that the enzyme possesses three distinct binding sites for these compounds. The active site appears to contain separate sites for …
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Effects of Levothyroxine Adminstration and Withdrawal on the Hypothalamic-Pituitary-Thyroid Axis in Euthyroid Dogs
… of total thyroxine (T4), free thyroxine (fT4) by equilibrium dialysis, thyrotropin (TSH), and 3,5,3'-triiodothyronine (T3) were measured every 4 weeks during supplementation and for 16 weeks after levothyroxine was discontinued. Results: Mean serum T4 and fT4 were significantly higher and TSH was …
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The binding of divalent cations to tobacco mosaic virus and to some isometric plant viruses
… their isolated proteins was investigated, using equilibrium dialysis and potentiometric titration, in an attempt to elucidate the role of divalent cations in virus stabilisation. It was found that dissociation of bound calcium ions from TMV is apparently a necessary but insufficient condition for …
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The Effects of Anesthesia and Surgery on Thyroid Function Tests in Dogs
… control group. Thyroxine (T4), free T4 (fT4) by equilibrium dialysis, triiodothyronine (T3), reverse T3 (rT3) and thyroid-stimulating hormone (TSH) concentrations were measured in all samples. Results: Results of all thyroid function tests were not significantly different between control and …
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Development of liquid chromatography mass spectrometry methods for the monitoring of ropivacaine, endogenous steroids and dihydroartemisinin
… free and bound ropivacaine in human plasma using equilibrium dialysis and HILIC chromatography coupled with a high resolution mass spectrometery. In this chapter a highly sensitive method for the determination of the free and bound portion of ropivacaine in patients undergoing knee and hip joint …
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INTERACTION AND PROTECTION OF ANTIMICROBIAL COMPOUNDS WITH CARBOHYDRATE-BASED COLLOIDAL SYSTEMS FOR IMPROVED FOOD SAFETY
… was used as the model antimicrobial peptide. Equilibrium dialysis experiments showed that PG-OS with different degree of substitution (DS) interacts with nisin in a Langmuir monolayer adsorption pattern in both non-emulsion and emulsion systems. The monolayer adsorption capacity (Qm) increased …
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INTERACTION AND PROTECTION OF ANTIMICROBIAL COMPOUNDS WITH CARBOHYDRATE-BASED COLLOIDAL SYSTEMS FOR IMPROVED FOOD SAFETY
… was used as the model antimicrobial peptide. Equilibrium dialysis experiments showed that PG-OS with different degree of substitution (DS) interacts with nisin in a Langmuir monolayer adsorption pattern in both non-emulsion and emulsion systems. The monolayer adsorption capacity (Qm) increased …
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Characterization of chemically fixed nitrogen-15-labeled liquid anhydrous ammonia and of biologically immobilized nitrogen-15 in typical Illinois mollisols
… and high molecular weight (HMW) components by equilibrium dialysis, and into generic FA and nonhumic substances by sorption-desorption from XAD-8 resin. The results show that: (1) chemical fixation by soil organic matter accounted for 36% of the total fixed NH$\sb3$; clay fixation accounted for …
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Dissolved Organic Matter Characterization and Impact on Mercury Binding in an Urbanizing Watershed
… constants (<em>K</em><sub>DOM’</sub>) using an equilibrium dialysis ligand exchange (EDLE) method. Molecular weights ranged from 668 Da (forest) to 209 Da (influent), SUVA<sub>254</sub> ranged from 3.17 (forest) to 0.02 (influent) L mg<sup>-1 </sup>m<sup>-1</sup>, and FI ranged from 1.2 (forest) …
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Evaluation of the Pharmacokinetic-Pharmacodynamic relationship, Metabolism and Plasma Protein Binding of the novel antitumor agent, 2-Methoxyestradiol (2ME2), following oral administration in patients with solid tumors.
… Plasma protein binding of 2ME2 was studied using equilibrium dialysis. Mean unbound fraction of 2ME2 (fu) in plasma of patients and healthy human volunteers was 0.019 ± 0.0043 and 0.027 ± 0.0019 respectively. Binding was concentration-independent and unaffected by presence of 2-methoxyestrone. …
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Clinical Pharmacology of MS-275, A Histone Deacetylase Inhibitor
… of MS-275 was characterized by a validated micro-equilibrium dialysis method. In vitro phase I and phase II hepatic metabolism of MS-275 were evaluated using human liver microsomes. A correlative covariate analysis was performed in an effort to explain the wide inter-individual variability among …
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A study of the binding of anti-aggregatory substances to platelet bound plasma proteins.
… with that obtained from the classical methods of equilibrium dialysis, dynamic dialysis, and continuous ultrafiltration and used to set up a number of nonlinear least squares regression analysis computer programmes to analyse binding isotherms. In the course of this study, the fluorescence methods …
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Effects of Furosemide and Oleic Acid On Thyroxine Binding to Isolated Pairs of Thyroxine Binding Serum Proteins
… acid (0.0 to 0.10 mol/L) were studied using equilibrium dialysis with purified binding proteins at concentrations 1/200 of levels in normal human sera, separated across the dialysis membrane and competing for T<sub>4</sub>.</p> <p>Furosemide (1.0 mol/L) reduced TBG bound T<sub>4</sub> in both …
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