Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Epiblast Stem Cells"”.
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Understanding transcriptional regulation through computational analysis of single-cell transcriptomics
… average gene expression across large pools of cells. In contrast, recent technological improvements have made it possible to perform expression profiling in single cells. Single-cell expression profiling is able to capture heterogeneity among single cells, which is not possible in conventional …
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Extracellular Signalling and Stem Cell Self-Renewal
… and modulating signalling in vitro allows for stem cell populations to be established from these lineages. Fibroblast growth factor (FGF) signalling triggers the differentiation of primitive endoderm (PrE) cells fated to contribute to the yolk sac, while cells unreceptive to FGF form the …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… mouse embryonic development. The ‘2i’ culture system using two small molecule kinase inhibitors was harnessed to convert lsh-/- mouse embryonic stem cells (mESCs) to a hypomethylated ‘ground state’ of pluripotency. Culture conditions were then altered to transition these ground state mESCs to …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… is pluripotency, the ability of embryonic cells of certain developmental stages to divide indefinitely without the loss of their potential to differentiate into derivatives of the three primary germ layers. Pluripotency has been captured in vitro by the establishment of cell culture …
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Effects of HOXB4 downstream targets on the haemopoietic differentiation of pluripotent stem cells
… of mouse ESCs with the recently derived epiblast stem cells (EpiSCs), thought to be comparable to hESCs. Haemopoietic colony forming assay and flow cytometry analysis showed that serum-free conditions generated 8- 10 fold more CD144+CD41+ and c-Kit+CD41+ haemopoietic progenitor cells …
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Studying the principles of cell identity transitions using naïve pluripotency induction as a model
… questions, I created defined and tractable systems based on reprogramming from epiblast stem cells to naïve pluripotency. By independent modulation of genetic and signalling parameters followed by isolation of successfully transitioning cells, I showed that naïve pluripotency can be …
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Date with Destiny: Genetic and Epigenetic Factors in Cell Fate Decisions in Populations of Multipotent Stem Cells
… problem. An important aspect of this is how cells exit a multipotent state and choose their fates in a correct manner and proportion. To tackle an aspect of this problem, I have focused on 2 multipotent models: one infinite self-renewal pluripotency in an artificial environment, and the …
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The role of SOCS3 in nuclear reprogramming and naïve pluripotency network integration
… deconvolute advanced biological properties that cells acquire through a series of hierarchical differentiation decisions. One of the primary unspecified states spanning a short period between conception and functional commitment of the cell is known as pluripotency. Pluripotency is the ability of …