Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Epiblast"”.
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NOGGIN Transport in a Model Human Epiblast
… I report the surprising discovery that in human epiblasts, NOGGIN and BMP4 were selectively secreted into opposite sides of the polarized epithelium, effectively segregating the ligand and its inhibitor into distinct extracellular spaces. Moreover, utilizing a microfluidic flow chamber, I …
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An in vitro model of human yolk sac and epiblast crosstalk
… primitive streak formation to the posterior epiblast, thereby establishing the anterior-posterior axis of the mouse embryo. Recent studies of human embryos cultured to postimplantation stages have alluded to the presence of a human equivalent of the mouse AVE in the anterior hypoblast, the …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… methylated stages of development (‘serum’ mESCs, epiblast stem cells and embryoid bodies). Implementation of this model system suggests that Lsh does not contribute to DNA methylation establishment in a pluripotent context, but rather is important for facilitating de novo DNA methylation during …
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A Study of Mouse Peri-Implantation Morphogenesis
… three lineages of the blastocyst, the embryonic epiblast and the two extraembryonic lineages, the trophectoderm and primitive endoderm. My first goal was to characterise the blastocyst to egg cylinder transition in mouse embryos and understand its mechanism through live imaging and inhibitor …
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Bovine Pre-gastrulation Development and the role Of Rauber's Layer
… is very different to the mouse, with a flat epiblast and the loss of the polar trophoblast, known as Rauber’s layer. In mice, the polar trophoblast is essential for the induction of gastrulation and the formation of the placenta. The pre-gastrulation layout of cattle embryos is shared by a …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… to the multilineage blastocyst, comprised of the epiblast (precursor of the fetus), hypoblast (precursor of the yolk sac) and trophoblast (precursor of the placenta). The blastocyst then implants into the maternal uterus and specifies body axes in preparation for gastrulation. It is thought that …
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Morphogenesis of the early post-implantation mouse embryo
… polar trophectoderm, a monolayer covering the epiblast of the blastocyst, which expands and undergoes invagination to form a slit-like cavity. By carrying out analyses of fixed specimens and live imaging of cultured embryos, I have found that the epiblast and ExE cavity extend towards each …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
During gastrulation, epiblast cells undergo an epithelial-to-mesenchymal transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic …
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Regulation of amnion induction during the human formative pluripotency transition
… Naïve hPSC resembles the pre-implantation epiblast, retain trophectoderm (TE) potential, but are resistant to somatic lineages induction. Primed hPSC is related to post-implantation epiblast and readily form germ layers but lack TE potential. The formative transition, corresponding to the …
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Regulation of polycomb repressive complexes at the neural differentiation gene Pax6
… progenitors of the stem zone/caudal lateral epiblast, termed neuromesodermal progenitors. Neural differentiation gene transcription begins in a sharply defined position due to loss of fibroblast growth factor (FGF) signalling. This highly coordinated onset of gene expression implies the …
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Date with Destiny: Genetic and Epigenetic Factors in Cell Fate Decisions in Populations of Multipotent Stem Cells
… cell population found in the Caudal Lateral Epiblast (CLE) of mammalian embryos, which contributes to the spinal cord and paraxial mesoderm. The aim here was to derive NMPs in vitro which exhibit similar gene expression patterns and function like their mouse embryo counterpart and study their …
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Extracellular Signalling and Stem Cell Self-Renewal
… sac, while cells unreceptive to FGF form the epiblast (Epi) that subsequently contributes to the embryo proper. In vitro, FGF signalling is required for preimplantation Epi-derived mouse ES cells to exit self-renewal. Conversely, in human ES cells and postimplantation Epi-derived mouse …
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Understanding transcriptional regulation through computational analysis of single-cell transcriptomics
… by analysing single-cell RNAseq data from the epiblast stem cells reprogramming and the leukaemia systems. Three different driver genes, namely Esrrb, Klf2 and GY118F, were shown to drive reprogramming of epiblast stem cells via different reprogramming routes. As for the leukaemia system, …
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Topographie molekularer Faktoren der Keimzellentwicklung während der frühembryonalen Entwicklung des Kaninchens
… 0 im Hypoblast exprimiert, erste "geblimpte" Epiblastzellen, die wahrscheinlichen PGC-Vorläufer, treten im Stadium 2 in der PGE auf, ihre Anzahl nimmt bis ins Stadium 4 zu, im Stadium 5 sind nur noch wenige nachweisbar. BMP2 bildet im Stadium 1 eine ringförmige Expressionsdomäne aus, die den …
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Molecular analysis of germ cell specification
… as a small population in the post-implantation epiblast in response to external signals, including BMP4 and WNT. However, what governs the first steps in the decision of cells to enter the germline remains unknown. Here we use single cell omics (scRNA-seq and scATAC-seq) to characterise the …
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Gene expression studies of pregastrulation development: the basement membrane is essential for cell differentiation
… are responsible for the induction of the epiblast EBs derived form embryonic stem (ES) cells, modified to repress FGF receptor signaling, have been described before. However, a full-scale analysis of the transcriptome was missing. We therefore analysed these EBs at four time points during …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… the pre-implantation mouse embryo, and primed Epiblast Stem Cells (EpiSCs), which are most similar to the post-implantation mouse embryo. In recent years, the exit of mESCs from naive pluripotency has been partially characterised, and several protocols have been developed that can generate …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… (as measured by STAT3 activation). However, some epiblast (NANOG+) cells were also IL6-responsive, and IL6 appeared to initially slow epiblast differentiation. Finally, IL6-treated blastocysts also had increased transcripts of hypoblast/primitive endoderm (PE) markers. These results indicate that …
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Studying the principles of cell identity transitions using naïve pluripotency induction as a model
… tractable systems based on reprogramming from epiblast stem cells to naïve pluripotency. By independent modulation of genetic and signalling parameters followed by isolation of successfully transitioning cells, I showed that naïve pluripotency can be established via distinct routes. These …
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
… Nanog is essential to establish the pluripotent epiblast. For Nanog -/somatic cells reprogramming by defined factors, in a culture condition using inhibitors for MEK/ERK and GSK3 (2i) fails to occur. This led to the assumption that acquisition of pluripotency in reprogramming is dependent on …
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