Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"EpiSCs"”.
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… mouse embryo, and primed Epiblast Stem Cells (EpiSCs), which are most similar to the post-implantation mouse embryo. In recent years, the exit of mESCs from naive pluripotency has been partially characterised, and several protocols have been developed that can generate either transient or …
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Effects of HOXB4 downstream targets on the haemopoietic differentiation of pluripotent stem cells
… with the recently derived epiblast stem cells (EpiSCs), thought to be comparable to hESCs. Haemopoietic colony forming assay and flow cytometry analysis showed that serum-free conditions generated 8- 10 fold more CD144+CD41+ and c-Kit+CD41+ haemopoietic progenitor cells (HPCs) compared to serum …
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
… post-implantation epiblast derived stem cells (EpiSCs) to nai:ve pluripotency. I showed that for reprogramming of adult neural stem cells, increased activation of JAK/ST AT3 enhances efficiency and acts as a potent reprogramming factor. In addition, I demonstrated that sufficient activation of …
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Date with Destiny: Genetic and Epigenetic Factors in Cell Fate Decisions in Populations of Multipotent Stem Cells
… protocols stresses that Epiblast Stem Cells (EpiSCs) are an effective source for deriving a multipotent population resembling the embryo Caudal Epiblast (CE), that generates NMPs. Furthermore, self-organization of this CE-like population, resulted in axially organized aggregates. Exploiting …
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The role of SOCS3 in nuclear reprogramming and naïve pluripotency network integration
… genes working as barriers for reprogramming from EpiSCs to ESCs. Having assembled a comprehensive lentiviral library and established a tractable cell system to target the mouse genome, I screened EpiSCs in the reprogramming setup which allowed selection of effective reprogramming antagonists. Here …
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Studying the principles of cell identity transitions using naïve pluripotency induction as a model
… Oct4 at this level is sufficient to reprogram EpiSCs and more advanced cell types under signal instruction alone, yet is also compatible with bonafide development when returned to the in vivo context. At this expression level, I therefore deem Oct4 to be a ‘transition’ factor, a novel concept …
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Determining the Signalling Pathways that Govern Human Naive Pluripotency
… state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived either through transient histone deacetylase inhibition mediated resetting of conventional hESCs or via isolation of the inner cell mass. …