Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Epac"”.
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Epac-mediated modulation of neurotransmitter release from cultured hippocampal neurons
… wurde die Beteilung des neuen cAMP Rezeptors Epac an der cAMP-vermittelten Regulation der Neurotransmission untersucht. Mit Hilfe des Patch-Clamp Verfahrens wurde die Neurotransmitterfreisetzung bei hoch oder niedrigfrequenter Stimulation kultivierter exzitatorischer autaptischer Nervenzellen …
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The role of extracellular matrix in modulation of myocyte Ca2+ signalling and contraction in the isolated rat heart
… from the adult rat hearts. The effect of novel EPAC signalling pathway which contributes to the EC-coupling and Ca2+ homeostasis was also investigated by activating with cpTOME (an EPAC agonist) in intact trabeculae. It was depicted that cpTOME do not affect trabeculae until the experimental …
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A Role For Epac1 and Epac2 In Nociceptor Hyperexcitability and Chronic Pain After Spinal Cord Injury
… remain limited or non-existent. The cAMP sensor EPAC has previously been shown to play a key role in chronic inflammatory and neuropathic pain, though the contribution from each of its two main isoforms, EPAC1 and EPAC2, is unclear. Here I test the hypothesis that both EPAC1 and EPAC2 play a key …
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The Non-genomic Signaling Pathways Mediated By G-protein Coupled Estrogen Receptor 1 (GPER) In Coronary Arteries
… exchange proteins directly activated by cAMP (Epac) as well as PKA; and 4) activation of GPER potentiates coronary artery contraction by a G??/EGFR-dependent pathway. Isometric tension studies were performed on endothelium-denuded porcine coronary arteries to test the function role of GPER and …
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Host response of lung cells to virulent Bacillus anthracis
… PKA and exchange protein activated by cAMP (Epac). Including PKA- and Epac-selective cAMP analogs confirmed the involvement of both pathways in this inhibition. This suggests that EdTx-generated cAMP weakens the host immune response by impairing cytoskeletal functions essential for MØ …
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The crosstalk between the ERK and the cAMP signalling pathways in PC12 Cells
… the Exchange protein activated by cyclic AMP (Epac) and the cAMP regulated protein kinase (PKA) respectively, which were hypothesised to be the effectors of cAMP in the regulation of ERK. The Epac agonist mimicked the effects of cilostamide on the activation of ERK, but failed to enhance cell …
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Investigating luteinizing hormone receptor signal transduction using the cyclic AMP reporter ICUE1
… relied on biochemical methods. ICUEl is an Epac-based cAMP reporter which undergoes conformational changes after binding cAMP. Unlike traditional biochemical assays, ICUEl combined with FRET techniques is capable of real-time monitoring of cAMP levels in individual cells. In this project, …
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The Role of Interleukin-6 in the Regulation of Glucose Sensing in Hypothalamic GT1-7 Neurons
… role for the exchange protein activated by cAMP (EPAC) implicated in this observation. The results obtained suggest that EPAC may mediate this effect by enhancing the sensitivity of ATP-sensitive potassium (KATP) channels to ATP-mediated inhibition following IL-6 exposure. The current results …
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Effect of Prostaglandin E2 on the Expression of Adhesion Molecules in Mouse Cerebrovascular Endothelial Cells
… ICAM-1 expression. However, involvement of Epac was confirmed by experiments using specific stimulators and inhibitors, and siRNA technology. PGE2 and dbcAMP stimulated phosphorylation of Akt and GSK-3β, suggesting the role of PI3K/Akt in PGE2-induced ICAM-1 expression. This inference was …
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The Roles of Rap1 in Cancer Metastasis and Pancreatic Islet Beta Cell Function
… demonstrated that the Rap activator proteins, Epac, are important regulators of pancreatic β-cell insulin secretion. However, the role of Rap1 in β-cell biology has not yet been defined. Here we established roles for Rap1 in distinct signaling events and begin to answer some of the …
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Role of Exchange Protein Directly Activated by cAMP Isoform 1 in Energy Homeostasis
… exchange protein directly activated by cAMP (Epac). Epac has two isoforms, the ubiquitously expressed Epac1 and the more tissue restricted Epac2. The biological functions of Epac1 have been revealed, including cardiac stress, chronic pain, cancer and infections. However, the roles of Epac1 in …
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EPAC1 AS A THERAPEUTIC TARGET FOR PANCREATIC DUCTAL ADENOCARCINOMA
… proteins, exchange protein activated by cAMP (EPAC), ushered in a new era in cAMP signaling. EPAC is composed of two main isoforms, EPAC1 and EPAC2, whose roles in mediating cAMP effects in various cellular contexts remains relatively unclear, and must be elucidated to examine the therapeutic …
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Role of Exchange Protein Directly Activated by cAMP Isoform 1 in Energy Homeostasis
… exchange protein directly activated by cAMP (Epac). Epac has two isoforms, the ubiquitously expressed Epac1 and the more tissue restricted Epac2. The biological functions of Epac1 have been revealed, including cardiac stress, chronic pain, cancer and infections. However, the roles of Epac1 in …
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Adenylyl Cyclase 2 Selectively Regulates IL-6 Expression in Human Bronchial Smooth Muscle Cells
… exchange protein directly activated by cAMP (Epac) predominated in mediating the IL-6 response, but at higher cAMP concentrations protein kinase A (PKA) assumed the larger role. IL-6 promoter mutations demonstrated that activator protein 1 (AP-1) and cAMP responsive element (CRE) transcription …
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cAMP and Calcium Interactions in Endocrine Cells
… cAMP molecular targets. 8-cpt-cAMP initiated an Epac mediated pathway and had strong effects on calcium influx in the cell; suggesting, a relatively strong cAMP target. Experiments of α-Pancreatic cells were done in 2mM glucose and 8mM glucose to stimulate hypoglycemia and resting states …
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Evaluating luteinizing hormone receptor signaling using the cyclic AMP reporter ICUE3
… to LH receptors. These studies used ICUE3, an EPAC-based reporter molecule for cAMP in cells. ICUE3 is an engineered molecule that contains a FRET donor and acceptor pair as well as a membrane-targeting sequence. FRET occurs when the donor fluorophore in an excited electronic state transfers …
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Funktionelle Charakterisierung des Vasodilatator stimulierten Phosphoproteins (VASP) für die Stabilität des Aktin-Zytoskeletts und die Integrin-abhängige Zelladhäsion
… Adhäsion von Zellen zu. Aktivierung von Epac, einem Guanin-Nukleotid- Austauschfaktor von Rap1, führt in Wildtyp-Zellen zur Verstärkung des Widerstandes gegenüber mechanischen Kräften, die auf Fibronektin-beschichtete Perlen wirken, ohne dabei die Membranrigidität zu verändern. Diese …
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Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
… pathways through its effectors including EPAC, PKA and ion channels. cAMP signalling regulates processes such as memory, muscle contraction and inflammatory responses. PDE enzymes offer a mechanism to negatively regulate elevated cAMP levels elicited by activators of adenylyl cyclase. …
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Acidosis Activated GPR68 Promotes Vascular Smooth Muscle Cell Growth
… cyclic AMP and its downstream effectors PKA and EPAC, yet the influence of GPR68 and its cyclic AMP signals on VSM cell (VSMC) growth in the context of CVD in not known. The hypothesis of this study was to determine that acidic activation of proton sensing GPR68 and its Gs signals regulates VSMC …
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Investigating Mechanisms In Nociceptors Driving Ongoing Activity and Ongoing Pain
… and downstream cAMP signaling via PKA and EPAC. Furthermore, whole-cell patch clamp recordings of primary DRG neurons, analysis of spontaneous pain behavior, and RNA sequencing were used to reveal an unexpected persistence of hyperexcitability in isolated DRG neurons and potentially …
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