Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 103 for “"Enzymology"”.
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Enzymology of Gentamicin Biosynthesis
Gentamicin C complex is a mixture of five structurally similar aminoglycoside antibiotics, gentamicins C1, C1a, C2, C2a, and C2b, produced by the actinomycete bacterium Micromonospora echinospora. It is established in clinical use and despite significant toxicity remains valuable to treat severe …
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Large scale quantum mechanical enzymology
… the system sizes required to study problems in enzymology. This dissertation presents methodological developments and protocols, including best practice, for accurate preparation and optimisation, combined with proof-of-principle calculations demonstrating reliable results for a range of small …
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Enzymology of DHHC-mediated Protein S-Acylation
Protein <italic>S</italic>-acylation is the post-translational modification of proteins with long-chain fatty acids at cysteine residues via a thioester linkage. The most commonly attached lipid is 16-carbon palmitate, thus the process is often called palmitoylation. Unlike other lipid …
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Enzymology of butanol formation in Clostridium Beijerinckii
The present study encompasses an investigation of the expression of solvent forming enzymes and purification and characterization of butanol-forming enzymes. More sensitive and accurate procedures for the determination of acids and solvents in cultures have been developed, which led to the …
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Structural enzymology of bacterial carbon fixation and storage
As concerns about sustainable energy and climate change grow, there has been an ever growing interest in understanding how Nature sequesters and uses carbon. In this thesis, I present X-ray crystal structures of two central players in bacterial carbon fixation and storage: carbon monoxide …
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Structural enzymology of soluble methane monooxygenase protein-protein interactions.
Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that activates molecular oxygen, breaks the 105 kcal/mol C-H bond of CH4, and inserts one atom of O to create methanol at ambient temperature and pressure. This feat of catalytic prowess requires all three protein components of …
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Discovery, enzymology, and engineering of bacterial natural product pathways
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Enzymology and inhibition of ghrelin acylation by ghrelin O-acyltransferase
<p>Ghrelin is a circulating peptide hormone that plays a regulatory role in many physiological processes related to energy balance and metabolism. To be recognized by its receptor, growth hormone secretagogue receptor 1a (GHSR1a), the serine 3 residue of ghrelin must be octanoylated. This unique …
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Enzymology of dicarboxylic acid metabolism in Corynebacterium sp. strain 7E1C
1.Dicarboxylic acid metabolism was investigated in the alkane-utilizing bacterium Corynebacterium 7E1C.2.The best yields of dicarboxylic acid were obtained during growth on dodecane although significant amounts of tetradecanedioic acid were produced during growth on methyl myristate. No …
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Comparative enzymology of wheat germ aspartate transcarbamoylase and related studies
Studies on aspartate transcarbamoylase from various organisms have been reviewed. The amino acid composition, isoelectric point and peptide maps of purified wheat germ aspartate transcarbamoylase have been elucidated and compared to the E.coli enzyme. Three types of essential amino acid residues …
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Studies in biochemistry--steroid synthesis, P-450 enzymology and renal lithogenesis
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis: Iron-Sulfur Biochemistry and Enzymology
The iron-sulfur prosthetic group of glutamine PRPP amidotransferase was found to be a {4Fe-4S} cluster. In the native enzyme, the cluster exists in the diamagnetic +2 redox state. Treatment of the cluster with oxidants resulted either in no reaction or in oxidative dissolution of the cluster, i.e., …
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The biosynthetic Claisen condensation : mechanistic enzymology of biosynthetic thiolase from Zoogloea ramigera
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1987.
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Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
<p>N5-Carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase), a key enzyme in microbial de novo purine biosynthesis, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to N5-CAIR. To date, this enzyme has been observed only in microorganisms, and thus, it represents an …
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Enzymology of lipid metabolism by CYP2J2 as controlled by multiple-ligand binding events
Lipid mediators are a recently discovered class of endogenous molecules that perform a variety of functions in the body. Specifically, polyunsaturated fatty acids (PUFAs) are transformed by three main pathways: the cyclooxygenase, lipoxygenase, and cytochrome P450 (CYP) epoxygenase pathways. …
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
Our laboratory is interested in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical …
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ENZYMOLOGY AND MOLECULAR BIOLOGY OF BILE ACID 7alpha- AND 7beta- DEHYDROXYLATION BY THE INTESTINAL BACTERIA CLOSTRIDIUM SCINDENS AND CLOSTRIDIUM HYLEMONAE
The collective microbial genomes within our gut(microbiome) represent a powerful metabolic force, leading many authors to call our GI flora an "organ within an organ", and the metagenomic sequencing of our microbiome, "the second human genome project". Bile acids, endogenously produced by the host …
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In Search of Catalytic Proficiency: The Importance of Enzyme Conformational Change to Orotidine 5'-Monophosphate Decarboxylase Catalysis
By applying the tools of enzymology learned in the Gerlt Laboratory and working successfully with numerous collaborators, I have furthered our understanding of the mechanism of one of Nature's best catalysts, OMPDC. Increasingly in enzymology, the role of conformational change in enzyme catalysis …
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Formylmethanofuran formation in Methanobacterium thermoautotrophicum
… is not measurable in vitro unless activated. The enzymology, and the activation of this reaction were studied.
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