Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Enzymes and Coenzymes"”.
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… net greenhouse gas emissions, create more jobs, and enhance national energy security. Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated chemical reaction by the in vitro assembly of numerous enzymes and coenzymes. Two of the biggest challenges for its …
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… net greenhouse gas emissions, create more jobs, and enhance national energy security. Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated chemical reaction by the in vitro assembly of numerous enzymes and coenzymes that microbes cannot do. One of the largest …
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Developing a vector for expressing serratiopeptidase in Lactococcus lactis
… inflammation include lifestyle changes (diet and exercise) and a variety of conventional drugs. Beyond these treatments, supplementing the body with systemic enzymes may attenuate inflammation. One promising enzyme is serratiopeptidase. Serratiopeptidase is a serine protease expressed by …
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Production and characterization of a phage endolysin with putative antimicrobial activity against Cutibacterium acnes
… Because of the heterogeneous pathology of acne and the increasing antibiotic resistance of the causal agent (Cutibacterium acnes), novel therapeutics need to be developed to treat acne. One option is endolysins, highly conserved enzymes from bacteriophages that disrupt the bacterial cell wall. …
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Identifying notch-regulated genes using a specific notch signaling inhibitor
… a balance between bone resorption by osteoclasts and bone formation by osteoblasts to maintain skeletal integrity. Disruptions to this equilibrium can lead to pathological conditions such as osteoporosis. Osteoclasts originate from macrophages, and their differentiation is regulated by several …
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Toward the Synthesis of Nuclease Models.
<p>Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general …
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Expression, Purification, and Characterization of the Mast Cell Proteases Chymase and Cathepsin G.
… with wound healing, allergies, inflammation, and defense against pathogens and have been detected in tissues close to blood vessels especially in the areas between the inside of the body and the external environment, such as the skin, lungs, digestive tract, mouth, and nose. Previous studies …
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Inhibition of Cysteine Protease by Platinum (II) Diamine Complexes
… drugs such as cisplatin, carboplatin and oxaliplatin are used in treatment. Cisplatin enters the cell through copper transporter CTR1 by passive diffusion and bind to DNA and proteins. Cisplatin is found to inhibit several enzymes targeting cysteine, histidine and methionine residues, …
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Interaction Between Two E3 ligases, NEDD8ylated Cullin and HHARI
… Most well known RBRs include Parkin and HOIP, which are associated with Parkinson’s disease and innate immune deficiency. However, it is not well known how the RBR proteins gain activity, as they are known to be autoinhibited. Here I show that a specific F430A, E431A, E503A triple …
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CDC45 Function Alters Cell Sensitivity to DNA Topoisomerase I Poisons
… replication, chromatid condensation and recombination. The enzyme binds duplex DNA and through a series of strand cleavage and religation reactions removes positive or negative supercoils relieving torsional strain. Top1 is the sole cellular target of the anticancer agent …
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The Impact of Treatment With Palynziq® On Quality of Life For Individuals With Pku
… recommended range (μmol/L). The time commitment and financial, emotional, and social burdens of adhering to a restrictive diet, as well as the neurocognitive symptoms associated with elevated Phe levels, make it challenging to manage PKU and lead to reductions in quality of life. …
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Periplasmic Modification of the 1-Phosphate Group of Lipid A in Gram-Negative Bacteria.
… (LPS) is important for the pathogenesis and virulence of various Gram-negative bacteria. The major lipid A species of <em>Helicobacter pylori</em> is significantly different from that of <em>Escherichia coli</em>. <em>H. pylori</em> lipid A contains fewer acyl chains and phosphate groups …
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An Immunochemical Analysis of Alcoholic Hyaline and its Relationship to Keratin Intermediate Filaments
<p>Alcoholic Hyaline (AH) and keratin intermediate filaments possess ultrastructural, biochemical and immunochemical similarities. This study compares human AH, human stratum corneum keratin (HSCK) and normal human liver cytokeratin (HCK) by quantitative immunochemical means, using polyclonal as …
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An Automated Syringe Pump System For Improving The Reproducibility of Dynamic Hyperpolarized Mri Phantoms
… its ability to provide good soft tissue contrast and image resolution without the use of ionizing radiation. The use of hyperpolarized pyruvate as a contrast agent for tumor metabolism during MR scans has the potential to provide information about tumor metabolism in vivo that is not available …
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Enzyme Architecture and Flexibility Affect DNA Topoisomerase I Function
… duplex DNA; a non-conserved linker domain; and a C-terminal/catalytic domain. Top1 catalyzes changes in DNA topology by transient cleavage of a single DNA strand and the concomitant formation of a phosphotyrosyl linkage between the enzyme and the 3’ DNA end. This covalent Top1-DNA complex is …
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… Oxysterol-related binding proteins ORP5 and ORP8 exchange PtdSer synthesized in the ER for phosphatidylinositol-4-phosphate (PI4P) synthesized in the PM. We show that depletion of ORP5 or ORP8 reduced PM PtdSer levels, resulting in extensive mislocalization of KRAS from the PM. …
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The Purification, Properties and Subunit Structure of Glycerol Dehydrogenase
… experiments with the enzyme treated with urea and dithiothreitol, and with dithiothreitol and p- hydroxymercuribenzoate in the presence of guanidinium chloride indicated that the isolated enzyme is composed of subunits. The untreated enzyme, with a molecular weight of 3.4 x 10<sup>5</sup> …
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Characteristics of the Recovery of the Coenzyme A-Synthesizing Protein Complex from Saccharomyces Cerevisiae
… formed by exposing the yeast cell to ether and dry ice. Only after solubilization has this multienzyme complex been shown to catalyze the formation of bound dephospho-CoA utilizing the substrates adenosine triphosphate, D-pantothenic acid and L-cysteine. A low molecular weight component or …
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The Molecular Basis of Fitness and Transmissibility of Neuraminidase Inhibitor Resistant Influenza A Viruses
… (NA) inhibitors including oral oseltamivir and inhaled zanamivir are among the first line of defense against influenza virus infection. Development of resistance to NA inhibitors is a huge drawback for limited options for the control of influenza. During the first decade of NA inhibitor use, …
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