Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Enzyme-linked immunosorbent assays"”.
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Development of novel Competitive Enzyme-linked immunosorbent assays to detect SARS-CoV-2-specific antibodies in animals
… detect the exposure of humans to the virus, few assays have the capacity to detect antibodies in a wide variety of species. Here, I describe the development of a competitive enzyme-linked immunosorbent assay (cELISA) to detect SARS-CoV-2 antibodies in mammals for which species-specific reagents …
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Analysis of the Immunomodulatory Properties of Mycobacterium Cell Wall Fraction on the Cytokine Production of Peripheral Blood Mononuclear Cells of Healthy Dogs
… blood mononuclear cells (PBMCs), using enzyme-linked immunosorbent assays (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantifying cytokine protein and mRNA transcripts, respectively. PBMCs were isolated from eight healthy dogs and cells were …
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Dendritic cell maturation and activation via RNA/DNA danger signals : co-delivery of protein antigen with siRNA or CpG DNA
… or siRNA as adjuvants, we observed via enzyme-linked immunosorbent assays for interleukin 12 and interferon-[alpha], respectively, that DCs were activated by CpG oligonucleotide- and siRNA-functionalized nanoparticles [approx.]10-fold more potently than by soluble CpG or siRNA ligands.
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The use of whole blood cell cultures as a model for assessing the effects of SeptilinTM on the immune system.
… WBC were incubated with the product. Enzyme linked immunosorbent assays were used to screen for IL-6, IL-10, and IFN? as biomarkers for inflammation, humoral immunity, and cell mediated immunity, respectively. Results show that the presence of SeptilinTM in LPS stimulated WBC has no …
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Nežindančių motinų pieno maistinių medžiagų koncentracijos pokyčiai po gimdymo ir veiksniai bei procedūros, turintys įtakos jo sudėčiai /
… human milk macronutrient and energy value. Enzyme-linked immunosorbent assays (ELISA) were performed for the analysis of lactoferrin and lysozyme concentration in human milk. In cooperation with Italian researchers, innovative human milk metabolome analysis was conducted; the analysis …
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Surface functionalisation of encoded SU-8 microparticles and their uses in multiplexed suspension biological assays
… for various multiplexed suspension biological assays. These microparticles, which are manufactured using photolithography of a commercial epoxy-based negative photoresist (SU-8), contain micrometre-sized diffractive elements and can encode millions of unique codes. In this thesis, the …
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Female Social Rank and Steroid Production in Semi-Free Ranging Lemur Catta on St. Catherines Island, Georgia
… Samples (n=15) were analyzed for steroids using enzyme-linked immunosorbent assays. Social status was determined via behavioral observations (50 hours). Due to the known negative relationship between cortisol and other steroids, its concentration was also analyzed to investigate a possible effect …
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Influenza C : a pilot study investigating the prevalence in Lancaster of a neglected respiratory virus
… chain reaction (PCR) and the serum samples by enzyme-linked immunosorbent assays (ELISA) and, of the samples tested via ELISA, 106 were positive (82%) and 23 were negative. Two swab samples also appeared positive for influenza C following PCR and so were sent for deep sequencing. A further …
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A Conserved epitope on the surface of carlaviruses
… relies heavily on double antibody sandwich-enzyme-linked immunosorbent assays (DAS-ELISA) for detection of plant pathogens. Each individual sample requires a separate test for each pathogen. The aim of this research was to simplify the procedure, which would result in considerable savings in …
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A photonic crystal biosensor application for assessment of iron deficiency
… analytical error was quantified. Commercialized enzyme-linked immunosorbent assays (ELISAs) served as the reference methods to quantify the analytical error of the PC biosensor measuring both biomarkers in the IA and SA format. In the SA human liver ferritin (450 kDa), clinical serum controls, …
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Proteomic Characterisation of the mdx-4cv mouse model of Duchenne Muscular Dystrophy
… have been further verified by biochemical assays including comparative immunoblotting and enzyme-linked immunosorbent assays. In-depth analyses have been performed on skeletal muscle, the aged heart, and brain tissue to investigate proteome-wide changes in the skeletal musculature, the …
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Early Diagnosis of Alzheimer's Disease and Studying Binding to Opioid Receptors using Whispering Gallery Mode Microtoroid Biosensors
… and specificity compared to traditional enzyme linked immunosorbent assays.
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The Effects of TET2-Deficiency on Neutrophil Gene Expression and Function
… LTF serum protein titers were quantified with enzyme-linked immunosorbent assays (ELISA). Additionally, the Tet2flox/flox Vav-iCre mouse model was used to investigate the effects of Tet2-deficiency on neutrophils. Motility and phagocytosis were assessed through a Staphylococcus aureus …
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POST-EXPOSURE TREATMENT OF MARBURG VIRUS ANGOLA DISEASE IN NON-HUMAN PRIMATES USING RECOMBINANT VESICULAR STOMATITIS VIRUS VECTORS
… cytometry. Humoral responses were assessed via enzyme-linked immunosorbent assays and plaque-reduction neutralization tests. In survivors, we found upregulation of STAT4, an early transcription factor associated with T helper 1 (Th1) and T follicular helper differentiation, and genes mapping to …
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An Immunochemical Analysis of Alcoholic Hyaline and its Relationship to Keratin Intermediate Filaments
… polyclonal as well as monoclonal antibodies. Enzyme-linked immunosorbent assays (ELISAs) were employed, as well as "immunoblotting" and immunocytochemical techniques. HCK was isolated using Triton X-100 and "high-salt” buffers and exhibited seven major polypeptide bands on SDS-PAGE (41-58 kd). …
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Isolation and characterisation of novel DNA aptamers against Mycobacterium tuberculosis biomarkers: new tools for tuberculosis diagnostics
… solution to identify patients with tuberculosis. Enzyme linked immunosorbent assays (ELISAs) were performed on concentrated tuberculosis patient urine to detect two M. tuberculosis biomarkers: lipoarabinomannan (LAM) and early secreted antigen-6 kDa (ESAT-6). Concentrating urine improved the …
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Cytokines regulation of chemokine and chemokine receptor in relation to multiple sclerosis.
… level, as determined by real time PCR and enzyme linked immunosorbent assays (ELISA), respectively. CCL2 binding to astrocytes was then determined to evaluate any autocrine action on astrocytes in a single astrocyte preparation. CCL2 bound constitutively and following cytokine treatment. …
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Multiplexed immunoassay development for precision medicine diagnostics and protein characterization using silicon photonic microring resonators
… result for functional diagnostics. Immunoassays are a powerful tool to quantitatively measure cytokine levels in biological solutions. While enzyme-linked immunosorbent assays have been the gold standard technique, multiplexed approaches have rapidly developed in response to the need for …
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Autoantibody Responses to Traumatic Brain Injury
… of ongoing neurodegeneration. The use of enzyme-linked immunosorbent assays to measure autoantibodies in this setting is assessed, but their signal is dominated by the magnitude of polyantigenic response, and therefore cannot accurately differentiate dominant responses to particular …
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Exploring molecular and cellular mechanisms underlying seizures in neurocysticercosis
… larvae produce acetylcholinesterases, as these enzymes have the potential to affect neuronal signaling by digesting the neurotransmitter acetylcholine. Ellman's assays, in situ acetylcholinesterase activity assays, and patch clamp electrophysiology reveal that both Taenia crassiceps and Taenia …
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