Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Enzyme stability"”.
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Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications.
… attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) …
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GLUCOSE/ OXYGEN-BASED BIOFUEL CELL FOR BIOMEDICAL APPLICATIONS: ELECTRODE DESIGNS INTEGRATING CARBON COMPOSITE
… current density generation, cofactor and enzyme stability is a major accomplishment of this research. The technology herein can serve as a departing foundation to engineer electrode designs that meet the criteria required for reagentless biofuel cells for implantable and extracorporeal …
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Development and Characterization of Reagent Pencils for Microfluidic Paper Based Analytical Devices
… pencil composition. Further, the effects of enzyme stability in the presence of poly(ethylene glycol) (PEG) is investigated.</p> <p>To show the versatility of reagent pencils, a novel reagent pencil incorporating a stimuli responsive polymer, poly(N-isporopylacrylamide) (PNIPAM) was …
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Activity of α- and β-amylase at low temperatures
… -0.4°, and -9.4°F) in systems with purified enzymes and in a system with sweet potato puree for different periods of time. In the systems with purified enzymes, commercially purified swine pancreatic α-amylase and sweet potato β-amylase at 0, 0.5, 1.0, and 1.5 μg/0.5 ml concentrations were …
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From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly
<p>The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We …
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Structural and Functional Studies of Biotin-Dependent Carboxylases
… concerns the exceptional diversity of metabolic enzymes and how they respond to their ligands and dynamic environments with remarkable precision. In humans, the family of biotin-dependent carboxylases holds important roles in intermediary metabolism. Recent years have witnessed significant …
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Production, purification and properties of Bacillus thuringiensis neutral protease
… paraspore (crystal) an extracellular proteolytic enzyme. This study concerns the conditions for production, the.purification and the properties of this enzyme. It was found in studies relating to production of protease that B. thuringiensis var. kurstaki (HD-1) produced a considerable quantity of …
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Enzymatic fuel cells via synthetic pathway biotransformation
Enzyme-catalyzed biofuel cells would be a great alternative to current battery technology, as they are clean, safe, and capable of using diverse and abundant renewable biomass with high energy densities, at mild reaction conditions. However, currently, three largest technical challenges for …
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The Effects of Zinc on Skeletal Alkaline Phosphatase Activity and Skeletal Tissues
… by which zinc influences growth. ALP, an ectoenzyme expressed in high levels in osteoblasts, is a zinc-containing metalloenzyme and is important in bone formation. Preliminary studies showed that low levels of zinc could increase ALP specific activity in human osteosarcoma, SaOS-2 cells. …
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The chromatographic separation of carbohydrate mixtures
… fructose in the presence of the dextransucrase enzyme. Conversions of over 80% were achieved at 4 hr sucrose residence times. The crude dextransucrase was purified using centrifugation, ultrafiltration and cross-flow microfiltration techniques. Better enzyme stability was obtained by first …
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Biochemical characterization of calmodulin isoforms and identification of calmodulin-binding proteins in Arabidopsis
… pathways. A combination of in vitro biochemical enzyme activation and molecular biological approaches were employed to tackle this question. First, CaM isoforms produced from E. coli expression systems were used to test their abilities to activate pea NAD kinase. There was a small, but …
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The functional analysis of XhLEA3-2 - a LEA_4 from the resurrection plant, Xerophyta humilis
… was assessed using a lactate dehydrogenase (LDH) enzyme stability assay. Transgenic expression of XhLEA3-2 in E. coli and tobacco was also investigated. In summary, this thesis demonstrates that XhLEA3-2: has typical LEA protein properties according to bioinformatic analyses, has two close …
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Bioaffinity-based Concept for Glyphosate Detection
… study optimizes parameters such as pH levels, enzyme concentrations, and substrate concentrations using the Ellman enzymatic assay. AChE activity is best maintained at 50 mU with 3 mM DTNB and 5 mM ACth, and storage at 4°C assures stability. The study also exposes glyphosate's inhibitory effect …
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Protein containing porous polymers for biocatalytic applications
… great deal of research conducted into the use of enzymes as catalysts for reactions conducted in non-aqueous media. Not only has it been shown that enzymes can function effectively in organic media, but also that the range of reactions that they can catalyse has been vastly increased. The major …
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Characterisation of the structural stability of transketolase under biocatalytically relevant conditions
The enzyme transketolase (TK; E.C. 2.2.1.1) from Escherichia coli occupies a pivotal place in metabolic regulation. TK catalyses the interconversion of sugars by transferring a two-carbon ketol unit from a ketose donor substrate to an aldolase acceptor substrate. It is also an important biocatalyst …
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Sviluppo di biosensori: modifiche di superfici elettrodiche e sistemi di immobilizzazione enzimatica
… developed using an anionic clay matrix (LDH) as enzyme support. The enzyme glucose oxidase (GOx) was immobilized on a layered double hydroxide Ni/Al-NO3 LDH during the electrosynthesis, which was followed by crosslinking with glutaraldehyde (GA) vapours or with GA and bovine serum albumin (GABSA) …
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Crosstalk of protein phosphorylation and lysine acetylation on 14-3-3 proteins and their applications in brassinosteroid signaling and photosynthesis
… center; acetylation of RbCL may affect enzyme stability and the activity of Rubisco holoenzyme. Our data indicated that lysine acetylation is functionally important in photosynthesis. In summary, we identified new roles of 14-3-3 protein phosphorylation in BR signaling and provided the …
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Directed evolution of amino acid dehydrogenases for biocatalysis of chiral amines
… become a powerful practical approach to study enzymes and optimize them to catalyze industrially relevant transformations. In this thesis, I applied this strategy to the engineering of amino acid dehydrogenases for biocatalysis of chiral amines, focusing on two crucial features for successful …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… (hot) and the suffix ‘ase’ is a general term for enzymes. The specific activity of the pure enzyme was estimated to be 38,000 proteolytic units per mg (PU mg ml⁻¹) at 75°C using casein as the substrate. The purified enzyme had a pH optimum of 9.5 with an isoelectric point of 8.9. Caldolase …
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Deoxyribophosphoaldolase of human erythrocytes: identification, purification and characterization
The enzyme, deoxyribophosphoaldolase, reversibly cleaves deoxyribose- 5-P to glyceraldehyde-3-P and acetaldehyde. Until this exposition, it was not known to exist in human erythrocytes. However, the enzyme was postulated to occur in erythrocytes in order to account for the synthesis of …
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