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Showing 1 to 13 of 13 for “"Enzyme purification"”.
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Development of a Yeast Transcription System Using a Saccharomyces Cerevisiae Enzyme Extract and a Drosophila Melanogaster Histidine tRNA Gene
… problem is directed toward the isolation of an enzyme from the yeast Saccharomyces pombe which endonucleolytically processes the 3' terminus of transfer ribonucleic acids. Plasmids containing tRNA genes were a gift from Dieter Soll, Yale University Department of Molecular Biophysics and …
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INTERACTION OF CIBACRON BLUE 3G-A AND RELATED DYES WITH NUCLEOTIDE-REQUIRING ENZYMES
… has become widely used as an absorbant for enzyme purification. Its mode of interaction with the proteins which bind to it is not entirely clear. One popular theory for proteins' attraction for the dye is that it is a specific structural probe for the supersecondary protein structure called …
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The production of clinical dextran using membrane processes
… also has several other potential uses including enzyme purification. A dextransucrase bioreactor was also developed. This preliminary investigation highlighted the problems involved with the development of a successful bioreactor for this enzyme system.
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Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… α-amidating monooxygenase (PAM) is the only enzyme that catalyses the C-terminal amidation of peptides in vivo through its two catalytic cores, peptidylglycine α-hydroxylating monooxygenase (PHM) and peptidylglycine αamidating lyase (PAL). The cost and limited quantities of the commercial PAM …
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Tyrosine Sulfation in A431 Cells
… activity, which opened a door towards enzyme purification Sulfation of proteins on tyrosine residues has recently been shown as a widespread occurrence. Most of the known tyrosine sulfated proteins are secretory proteins. The tyrosine sulfotransferase activity was suggested to be …
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A Novel Method to Detect Bacterial Restriction-Modification (R-M) Systems
… These findings show that numerous restriction enzymes abundant in bacteria have yet to be discovered. Traditionally, restriction enzymes have been discovered by the classical restriction and modification (R-M) phenomena of bacteriophages (type I and III enzymes), or by direct enzyme assays …
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Characterisation of fibrinogen and fibrin proteolysis by the neutrophil membrane
… and fibrin degradation products. Whilst enzyme purification procedures were unsuccessful, several observations made during these attempts suggested that the neutrophil membrane associated proteolytic activity displayed similar characteristics to proteases of the azurophil granule. The …
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Purification of Aspartate Transcarbamoylase from Moraxella (Branhamella) catarrhalis
The enzyme, aspartate transcarbamoylase (ATCase) from Moraxella (Branhamella) catarrhalis, has been purified. The holoenzyme has a molecular mass of approximately 510kDa, harbors predominantly positive charges and is hydrophobic in nature. The holoenzyme possesses two subunits, a smaller one of 40 …
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Purification and Properties of Bacteriophage f2 Replicase
The present dissertation concerns the purification and properties of an RNA-dependent ribonucleotide-polymerizing enzyme (RNA replicase) produced during infection of E. coli with the RNA bacteriophage f2. Studies on the RNA replicase of the distantly-related bacteriophage Q3 have established the …
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Purification and characterization of fungal β-glucosidase enzymes
… applications are based on the utilization of enzymes. There are multiple cellulolytic enzymes required in the efficient hydrolysis of lignocellulose, and one of the key enzyme group is β-glucosidases. These enzymatic systems are mainly adopted from wood-decaying fungi. The overall enzymatic …
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The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels
… Chloroplasts, CF0F1, is an integral membrane enzyme, which catalyses the synthesis of ATP from ADP and Phosphate. The reaction is driven energetically by proton-transport across the enzyme. The activity of the enzyme is regulated through a transmembrane pH-difference and a redox process, in …
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Reinigung und Charakterisierung der alpha-Methylacyl-CoA-Racemase aus menschlicher Leber
Im Katabolismus methylverzweigter Fettsäuren spielt die alpha-Methylacyl-CoA-Racemase eine wichtige Rolle, indem sie die (R)- und (S)-Isomere von alpha-methylverzweigten Fettsäuren als Coenzym A Thioester racemisiert. Methylverzweigte Fettsäuren entstehen beim Abbau von Isoprenoiden und werden …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… characterize the substrate specificity of this enzyme along with its human homolog cathepsin C. Although native purification of DPAP1 is possible, the amount of purified enzyme obtained is insufficient for extensive biochemical characterization. To overcome this obstacle, a strategy was …