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Showing 1 to 15 of 15 for “"Enzyme kinetic"”.
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The Comparison of Rat and Human Intestinal and Hepatic Glucuronidation of Enterolactone Derived from Flaxseed Lignans
… express UDP-glucuronosyltransferases (UGT), the enzymes responsible for the conjugation of glucuronic acid to EL. To better understand the contribution of liver and intestine to the first-pass glucuronidation, I conducted an in vitro enzyme kinetic analysis of EL glucuronidation using hepatic and …
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Salivary Biomarkers of Acute Stress and Insulin Sensitivity in Nonhuman Primates
… (Eulemur mongoz). Hormones were analyzed with enzyme kinetic or immunoassays. Our study validated tools for analyzing alpha-amylase, cortisol, and insulin in saliva for those species. No significant relationship was found between sAA and insulin. Cortisol and sAA were highly correlated in …
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Renal dopamine and salt-retaining states
… in activity in the diabetic group was observed. Enzyme kinetic analysis of ASA showed that this was not due to the existence of an isoform having an altered affinity for dopamine sulphate. This rather paradoxical situation, that urinary-dopamine is decreased while ASA activity is increased, may …
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Methodologies and analysis of metabolic flux in mammalian systems
… on assumptions such as metabolic objectives or enzyme kinetic parameters. However, most current 13C-MFA methods have limitations on model scope, algorithmic efficacy and user interaction, especially given the availability of modern mass spectrometry-based metabolomics techniques and …
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Regulation of Ciz1 by Cyclin A-Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
… is known to be a substrate of cyclin A-CDK2, enzyme kinetic studies were performed to evaluate whether there is preferential phosphorylation at specific sites within Ciz1. We characterise new phospho-specific antibodies that will be of benefit in future in in vivo studies evaluating the role …
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Development and Characterization of a Stopped-Flow-Bypass Analysis System With Applications To Biochemical Measurements
… and for assays which utilize equilibrium and/or kinetic methods to create measurable product(s) for ultraviolet/visible spectrophotometry, fluorimetry, and chemiluminescence. Enzyme activities and fundamental enzyme kinetic parameters (Kms, Kis, VMAXs) were determined directly. Genetic …
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Polianilino ir pilipirolo, aukso nanodalelių bei įvairių elektronų pernašos tarpininkų taikymas gliukozės biologiniuose jutikliuose /
… the solution of glucose, oxygen and dissolved enzyme - catalyzed reaction that formed a strong oxidizing agent hydrogen peroxide and gluconic acid which allows the initiation of polymerization reaction process. Adapted polymer synthesis method demonstrated the possibility of polyaniline and …
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Polianilino ir polipirolo, aukso nanodalelių bei įvairių elektronų pernašos tarpininkų taikymas gliukozės biologiniuose jutikliuose /
… the solution of glucose, oxygen and dissolved enzyme - catalyzed reaction that formed a strong oxidizing agent hydrogen peroxide and gluconic acid which allows the initiation of polymerization reaction process. Adapted polymer synthesis method demonstrated the possibility of polyaniline and …
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Population structure, biochemical adaptation and systematics in temperate marine fishes of the genera Arripis and Chrysophrys (Pisces: Perciformes)
… distinct snapper populations were detected. Enzyme kinetic studies were carried out on snapper isocitrate dehydrogenase (IDH) and salmon malic enzyme (ME) allozymes to determine whether or not functional differences existed in response to temperature fluctuation. It was found that differences …
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Population structure, biochemical adaptation and systematics in temperate marine fishes of the genera Arripis and Chrysophrys (Pisces: Perciformes)
… distinct snapper populations were detected. Enzyme kinetic studies were carried out on snapper isocitrate dehydrogenase (IDH) and salmon malic enzyme (ME) allozymes to determine whether or not functional differences existed in response to temperature fluctuation. It was found that differences …
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Structural requirements for enzymatic efficiency in cofactor-independent decarboxylation
… decarboxylase (OMPDC) is the last enzyme in the de novo pyrimidine biosynthetic pathway. It catalyzes the decarboxylation of orotidine 5’-monophosphate (OMP) to uridine 5’-monophosphate (UMP), without the requirement of a cofactor. It provides catalytic rate enhancements of ~1017, …
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Mechanisms and measurements of sialidase in pneumonia
… susceptible to reinfection by encoding three enzymes: NanA, NanB and NanC where NanA is the focus of this study. NanA cleaves 3'- sialyllactose or 6'-sialyllactosefound on the host cell walls to release N-acetylneuraminic acid (sialic acid) and lactose. This aids the bacterial adhesion which …
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Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex: e1 and e2 substrate specificty, e1 carboligase activity, and e2 interchain succinyl transfer
… coli (E. coli) 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc) contains three components: a thiamin di phosphate (ThD P) dependent 2-oxogl utarate dehydrogenase (E1 o), a di hydrol i poylsucci nyl transferase (E2o), and a di hydrol i poyl dehydrogenase (E3). The first two components carry …
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Funktionelle Charakterisierung von prokaryotischen und eukaryotischen Molybdoflavoenzymen
Die Xanthin-Dehydrogenase aus Rhodobacter capsulatus ist ein cytoplasmatisches Enzym, welches ein (αβ)₂ Heterotetramer mit einer Größe von 275 kDa bildet. Die drei Kofaktoren (Moco, 2[2Fe2S], FAD) sind auf zwei unterschiedlichen Polypeptidketten gebunden. So sind die beiden spektroskopisch …
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PEDOT Electrodes as Redox Mediators for Determination of Antioxidants in Beverages
… was required for the next study involving an enzyme. In order to overcome this problem, two types of solutions were tested; the first one was an aqueous solution containing EDOT, LiClO4 and sodium dodecyl sulphate (SDS) and the second one had different ratios of acetonitrile/water containing …