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Showing 1 to 20 of 70 for “"Enzyme assays"”.
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Studies of Enzyme Kinetics and Inhibition Through Capillary Electrophoretic Enzyme Assays
Enzyme assays are used throughout the biological, chemical, and medical sciences for the study of enzymes, and typically are performed by bulk solution measurements in cuvettes or microplates. The development of new enzyme assays that combine the measurement of reaction rates with separation …
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Extracellular enzyme assays of Blastomyces dermatitidis and HeLa cells in combined and separate populations
Thesis (M.S.)--Michigan State University. Dept. of Microbiology and Public Health,
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Determining the Usefulness of Aerobic and Anaerobic Enzyme Assays as Proxies for Rockfish Ecological Data.
… fall, winter, and spring) using metabolic enzyme assays. Enzymes used were lactate dehydrogenase (LDH), malate dehydrogenase (MDH), pyruvate kinase (PK), and citrate synthase (CS). Some muscle composition data (percent water, percent protein, percent lipid, and protein as a percentage of …
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DEVELOPMENT OF A LC/MS/MS ENZYME METHOD FOR N8 - ACETYLSPERMIDINE MEASUREMENTS IN ENZYME ASSAYS
… the N<sup>8</sup> - acetylspermidine deacetylase enzyme activity. The method created in this thesis emphasizes sensitivity, accuracy and safety.</p> <p>In this study, HeLa cells were cultured and extracted to yield a crude N<sup>8</sup> - acetylspermidine deacetylase enzyme mixture. By measuring …
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Drug resistance mechanisms and drug design strategies for human immunodeficiency virus and hepatitis c virus proteases
… the efficacy the drugs. In this study, the enzyme assays indicate that darunavir and tipranavir exhibit the most potent inhibition against the multi-drug resistant (MDR) HIV-1 protease, which is supported by the co-crystal structures of the MDR protease-darunavir complex and the MDR …
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PaaG and PaaF: New Members of the Crotonase Superfamily
… proteins expressed, and purified. I showed by enzyme assays using purified proteins that beta-oxidation in phenylacetate metabolism proceeds via adipyl-CoA intermediates. I showed that PaaG catalyzes the isomerization of trans-Delta 3-hexenedioyl-CoA to trans-Delta2-hexenedioyl-CoA, uses an Asp …
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Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes
… be achieved through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific …
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Development of a phosphotriesterase enzyme assay for determination of organophosphate pesticides
… in the development of novel phosphotriesterase enzyme assays to measure paraoxon and other representatives of the organophosphate pesticides. The assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. This enzyme, which was labeled with fluorescein …
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Mass spectrometric analysis of chitooligosaccharides and their interaction with proteins
… homologes and isomers are applied to biological assays. Chitooligosaccahrides form high-affinity non-covalent complexes with HC gp-39 (human cartilage glycoprotein of 39 kDa). The affinity of the chitooligosaccharides depends on DP, FA and the sequence of glycosamin and N-acetylglycosamin …
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Increased expression of NADP malic enzyme in the guard cells of <i>Arabidopsis</i> plants
… of the transgene in these transformants. Enzyme assays shows higher ME activity in these transformants, indicating an active form of maize ME. Such transgenic plants would help to determine effects of increased ME activity on plant water loss.</p>
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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Structural and biochemical studies of plant inositol phosphate kinases
… able to form a complex with these substrates. Enzyme assays revealed that neo-inositol 1,3,4,6-tetrakisphosphate and D-chiro-inositol 2,3,4,5-tetrakisphosphate are phosphorylated twice by AtIPK1, producing neo-inositol hexakisphosphate and D-chiro-inositol hexakisphosphate. The enzyme was shown …
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Isothermal Titration Calorimetry Uncovers Substrate Promiscuity of Bicupin Oxalate Oxidase
… (CsOxOx) is a manganese-dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to form two moles of carbon dioxide and one mole of hydrogen peroxide. CsOxOx is the first oxalate oxidase reported to have a two beta barrel architecture (bicupin). The CsOxOx …
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Characterizing the oligomeric structure and catalytic activity of the dihydroorotase and aspartate transcarbamoylase from the bacterium, <i>bacillus anthracis</i>
… for survival. The dihydroorotase domain is a key enzyme in pyrimidine biosynthesis and a promising drug target. The genes encoding the dihydroorotase (DHOase) and aspartate transcarbamoylase (ATCase) of <em>Bacillus anthracis</em> (<em>B. anthracis</em>) were cloned for expression in …
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Resistance evaluation and management of Colorado potato beetle, Leptinotarsa decemlineata (Say), using novel chemistries
… tolfenpyrad on L. decemlineata. In leaf-dip assays, tolfenpyrad was highly toxic to L. decemlineata with LC50 values of 0.013 and 0.164 g ai/L for larvae and adults, respectively. Tolfenpyrad was also toxic to eggs with 0% hatching after being dipped in a field rate concentration. In field …
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Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha
… yeast and tobacco, in conjunction with in vitro enzyme assays. We discover two cytochromes P450 (CYP87G1 and CYP749B2) and a short-chain dehydrogenase (SDH2) responsible for formation of the lactone ring on the sterol side chain, a key chemical feature of withanolides. Two additional P450s …
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Preparation of brush border and basolateral membrane vesicles from bovine intestine for nutrient uptake studies
… gradient centrifugations. Membrane marker enzyme assays were used to monitor the effectiveness of the fractionation procedure. Enrichments were determined by comparing the enzyme specific activities of the membrane fractions to the homogenate. Sodium-potassium adenosine triphosphatase and …
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Characterization of Value Added Proteins and Lipids form Microalgae
… in Amphora sp. it was 39.89 ±2.09 % of dry mass. Enzyme assays were conducted spectrophotometrically. Nannochloris sp. had malate dehydrogenase, peroxidase and catalase activities. Amphora sp. exhibited malate dehydrogenase, catalase and cytochrome C oxidase activities. These enzymes have several …
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Human alkaloid biosynthesis : chemical inducers of Parkinson's disease?
… its biological function through a series of enzyme assays. The assays used to evaluate enzyme activity were modeled after the Pictet-Spengler condensation catalyzed by the plant enzyme. Substrates were selected based on their potential to form alkaloids previously identified in central …
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Aggregation Pheromone Biosynthesis and Engineering in Plants for Stinkbug Pest Management
… pheromones. Knowledge of the genes and enzymes involved in pheromone biosynthesis may allow engineering of pheromone biosynthetic pathways in plants to develop new forms of trap crops and agricultural practices for pest management. The harlequin bug, Murgantia histrionica, a specialist …
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