Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Enzyme Promiscuity"”.
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The role of enzyme promiscuity in plant metabolic evolution
… of these arose as extensions of pre-existing enzymes and metabolites, leveraging promiscuous enzymatic activities and opportunistic gene duplication events to produce novel metabolic traits. The goal of this dissertation is to examine the mechanisms of enzyme promiscuity with regard to the …
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Expanding the structural diversity of the RiPP class of natural products
… modifications installed by biosynthetic enzymes. These conformational restrictions allow these natural products to exhibit a wide range of therapeutic activities like antifungal, antibacterial, allelopathic, and antiviral. Despite the structural and functional disparities, they all share …
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Human Genetics, Psychotropic Drugs, and Acts of Violence
… or the interaction between the drugs and the enzymes that are primarily responsible for their metabolism (cytochrome P450s) could cause these reactions. Given that acts of violence could be medication-induced, the role of the rate of drug metabolism is discussed. Genetic testing of certain …
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Microbial synthesis of 3,4-dihydroxybutyric acid, 3-hydroxybutyrolactone and other 3-hydroxyalkanoic acids
… with new substrates, taking advantage of natural enzyme promiscuity for the stereospecific synthesis of a variety of 3HAs of desired carbon chain length and substituents. Using this platform, we have now demonstrated the synthesis of five novel products: 3,4-dihydroxybutyric acid (3,4-DHBA) and …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, halogens, and other moieties to endow biological functions and provide their producing organisms an evolutionary advantage. This thesis focuses on mechanistic and functional studies of such …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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Rationalising and accelerating enzyme engineering by quantitative sequence-function profiling in microdroplets
Enzymes catalyse nearly all reactions in Nature's vast chemical repertoire with high precision and under sustainable conditions. To apply them in cost-effective and sustainable biocatalytic production processes in chemical industry, enzymes first need to be modified or "engineered" to work with …
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Exploring the emergence of pesticide degradation in the α/β hydrolase superfamily
… triad of synaptic acetylcholinesterase. Numerous enzymes from different evolutionary origins have rapidly evolved to hydrolyse phosphotriesters since. Interestingly, all known enzymes have convergently reached the same mechanistic solution by recruiting divalent cations as a cofactor for metal-ion …