Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Enzyme Hydrolysis"”.
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Compounds Released from Biomass Deconstruction: Understanding Their Effect on Cellulose Enzyme Hydrolysis and Their Biological Activity
… during biomass pretreatment on cellulolytic enzyme was investigated. Liquid prehydrolyzates were prepared by pretreating switchgrass using 24 combinations of temperature, time, and sulfuric acid concentration based on a full factorial design. Temperature was varied from 140°C to 180°C; time …
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Steam Explosion Pretreatment of Cotton Gin Waste for Fuel Ethanol Production
… pretreatment, cotton gin waste was subjected to enzyme hydrolysis using Primalco basic cellulase. The sugars released by enzyme hydrolysis were fermented by a genetically engineered Escherichia coli (Escherichia coli KO11). The effect of steam explosion pretreatment on ethanol production from …
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Effect of Foliar Nitrogen and Sulfur Applications on Aroma Profile of Vitis vinifera L. cv. Petit Manseng using Modified Quantitative Descriptive Analysis, SPME GC-MS and Electronic Nose Technology
… content. Harvest samples underwent acid or enzyme hydrolysis of precursor fractions. Solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC/MS) analysis identified 27 free aroma and flavor compounds and 52 bound compounds. Lactones and carboxylic acids were the major …
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Miscanthus conversion to ethanol: Effect of particle size and pretreatment conditions for hot water
… from the recalcitrance of biomass to enzymatic hydrolysis, which necessitates an expensive pretreatment in combination with a heavy enzyme dosage. The objective of this study was to develop an e fficient process for conversion of Miscanthus x giganteus to ethanol using hammer milling for …
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Isolation and characterization of ferulic acid carbohydrate esters from corn hull (Zea mays)
… by analysis of the products of acid, alkali, and enzyme hydrolysis, in combination with carbon-13 and 2D proton NMR spectroscopy. Three feruloyl oligosaccharides were identified as 5-0-(trans)-feruloyl-L-arabinofuranose (FA), 2-0-β-xylopyranosyl-(5-0-trans-feruloyl arabinofuranose) (FXA), and …
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Application of physicochemical and biological pretreatments to enhance volatile fatty acids production via Dark Fermentation from agri-food waste
… the Doctoral Thesis project, the application of Enzyme Hydrolysis (EH) was also tested to enhance the valorization strategy. So, the general objective can be expressed as: "To develop and optimize physicochemical and biological pretreatments applied to agro-food residual biomass to favor the …
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Effects of corn quality and storage on dry grind ethanol production
… ethanol concentrations. Effects of different enzyme treatments on mean ethanol concentration over a year were evaluated. Two liquefaction enzymes (optimum pH – 5.8 and 5.1, respectively), two saccharification enzymes (optimum pH – 5.0) and one protease were used in five enzyme treatments (I, …
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Anticancer Potential of Soy Peptides
… than glycinin after simulated gastrointestinal enzyme digestion. Three topoisomerase II inhibitory peptides, FEITPEKNPQ, IETWNPNNKP and VFDGEL, were identified. Their interaction energies with CTD enzyme domain ranged from -186 to -398 Kcal/mol, as determined by molecular docking, correlated …
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The role of acid phosphatases in the phosphorus nutrition of arctic tundra plants
… differences among treatments, suggesting that enzyme activity would not provide a reliable measure of plant or soil phosphorus levels. Experiments with radiolabeled inositol hexaphosphate showed that Eriophorum is able to hydrolyze and absorb inorganic phosphate from an organic phosphate …
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Water Vapor Sorption by Soy Proteins, Proteinates and Hydrolysates
… that reduce the ability of an ion to sorb water. Enzyme hydrolysis increased water sorption. Each hydrolysed peptide bond increased water sorption by the same amount. The total amount of water sorbed by a hydrolysate was the sum of the water sorbed by the substrate, by the added Na ions, and by …
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Fermentable Carbohydrate Substrates Generated from Cereal and Pseudocereal Insoluble Dietary Fibers and Their In Vitro Fecal Fermentation
… of microwave treatment and sequential enzymatic hydrolysis in order to effect solubilization and improve fermentability. Characterization of the dietary fibers revealed that, besides cellulose and lignin, insoluble fiber from pseudocereals consisted of pectic polysaccharides and xyloglucans, and …
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Preprocessing of Biomass Using Mechanical, Chemical and Microbial Techniques
… optimizing the pretreatment temperature and enzyme requirements, and by developing a mechanical method to identify the heterogeneous fractions of switch grass. Switch grass was preprocessed with the fungus Phanerochaete chrysosporium and enzyme profiles were determined for various cellulase …
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Increasing dry grind ethanol yield through fiber pretreatment after liquefaction
… for dry grind plants. Recalcitrance towards enzyme hydrolysis is a major challenge associated with the conversion of corn fiber into ethanol. Objectives of this study were to study the effects of hot water pretreatment, disk milling and cellulase dose on conversion of corn fiber into ethanol …
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EVALUATION OF CELLULOLYTIC ENZYMES FROM A NEWLY ISOLATED BREVIBACILLUS SP. JXL; AND OPTIMIZATION OF COSLIF PRETREATMENT VARIABLES OF SWEET SORGHUM BAGASSE USING A RESPONSE SURFACE METHOD
… of simple sugars after a sequential pretreatment-enzyme hydrolysis process. Accordingly, changes in delignification, total reducing sugar (TRS) yield, glucan retention, digestibility and overall sugar yields resulting from various combinations of reaction times and temperatures were determined. …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… (hot) and the suffix ‘ase’ is a general term for enzymes. The specific activity of the pure enzyme was estimated to be 38,000 proteolytic units per mg (PU mg ml⁻¹) at 75°C using casein as the substrate. The purified enzyme had a pH optimum of 9.5 with an isoelectric point of 8.9. Caldolase …
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Structural changes to Pinus radiata wood lignin during kraft pulping and bleaching
… The method involves a three day enzymatic hydrolysis of ball milled wood, followed by a second ball milling stage. A combination of extraction and purification steps enables isolated yields of approximately 75% of the lignin present in the wood to be obtained. The key step is the second …
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Understanding the Relative Importance of the Effects of Soluble and Insoluble Inhibitors on the Enzyme Digestibility of Steam Pretreated Radiata Pine
… the sugar pathway. This pathway involves using enzymes to hydrolyse the carbohydrate polymers in the biomass to monomeric sugars, and then converting these sugars to ethanol. High sugar yields during enzymatic hydrolysis requires an effective pretreatment to disrupt and/or remove some of the …