Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"Enzyme Function"”.
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Statistical and Relational Learning for Understanding Enzyme Function
Unravelling the functioning of the complex processes involved in living systems is a challenging task. Enzymes are involved in almost all of the chemical processes taking place within the cell. They accelerate chemical reactions by forming a complex with the substrate and therefore lowering the …
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
… acid sequence make it clear that although the enzymes in this superfamily are structurally and mechanistically related, the chemistry and substrates are varied. Two highly divergent enzymes from Thermotoga maritima and Enterococcus faecalis were targeted for characterization through a …
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The chemistry and evolution of enzyme function: isomerases as a case study
… However any attempt to understand how protein function changes during evolution benefits from consistent definitions of function and robust approaches to quantitatively compare them. The function of enzymes is described as their ability to catalyse biochemical reactions according to the Enzyme …
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Harnessing Deep Learning with Protein Language Models to Unveil Microbial Enzyme Function in Health and Disease
… genomics, accurate annotations of the biological functions of enzymes are critical, as these proteins have important roles in catalysing essential biochemical reactions with high specificity and efficiency. Historically, functional annotation tools have relied on hidden Markov models (HMMs) that …
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Conserved Conformational Dynamics Control PTP1B Enzymatic Activity
… long-accepted paradigm of biochemistry is that enzyme function depends on the three-dimensional (3D) structure. However, this paradigm is changing. Namely, the emerging view is that protein function also depends on intrinsic protein dynamics. Further, while sequence conservation is …
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Cytochrome AA(3) From Rhodobacter Sphaeroides: Affinity Purification and Biophysical Characterization of Site-Directed Mutants
… protein which were predicted to be important for enzyme function by sequence analysis. Methods ranging from visible and vibrational spectroscopy to rapid kinetic measurements of electron and proton transfers were used to probe the various effects of these mutants on the structure and function of …
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Cytochrome AA(3) from Rhodobacter sphaeroides: Affinity purification and biophysical characterization of site-directed mutants
… protein which were predicted to be important for enzyme function by sequence analysis. Methods ranging from visible and vibrational spectroscopy to rapid kinetic measurements of electron and proton transfers were used to probe the various effects of these mutants on the structure and function of …
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Protein Design at Library Scale
… feasibleto create proteins with novel functions, driven by rapid progress in both com- putational modeling and high-throughput experimentation. Modern tools can explore vast sequence-structure spaces and evaluate biomolecular interactions, while experimental assays can now screen …
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The Molybdenum Cofactor: Modeling the Swiss Army Knife of Metabolic Diversity
… in mononuclear molybdenum (Mo) and tungsten (W) enzymes, continues to intrigue us. The complexity of this ligand has been suggested as a cog in Mo and W enzyme function, and the pterin has been increasingly appreciated for its electronic contributions. To elucidate the conundrum of pterin …
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Cooperativity and communication in archaeal Cdc48·20S, an ancient proteolytic machine
… their activities. A regulatory N domain functions to control the activity of the enzyme and binding to its partner peptidase, the 20S proteasome. In this thesis I present experiments which investigate the role of inter-ring communication in ATP hydrolysis, protein unfolding, and …
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The Effects of Cell Culture Oxygen Levels on the Replicative Senescence Processes of Primary Human Fibroblasts
… TCA precursor molecules due to attenuation in enzyme function; this theory was also supported by an observed up regulation of gene expression as a compensatory mechanism. Mitochondria exhibited changes in membrane potential as well as volume and percent volume per cell which suggested …
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Benzo[a]pyrene effects on Fundulus heteroclitus reproductive endpoints
… hydrocarbon (PAH) that modulates aromatase enzyme function and, thus, potentially interrupts normal reproductive function. The aim of this study was to use a fish model, Fundulus heteroclitus, to assess whether BaP exposure adversely impacted reproduction. Adult fish were exposed to …
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Conservation of Electrostatically Mediated Function Across Protein Families and Superfamilies
The molecular association between an enzyme and substrate is highly specific. The overall association reaction is a delicate balance between attractive and repulsive forces. Association occurs at the cost of removing waters from the binding site and reducing side chain degrees of freedom, which is …
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Neural and Behavioural Consequences of Chronic Inflammation following Spinal Cord Injury
… kinetics, as well as indirectly by altering enzyme function thereby resulting in changes in critical neuroactive compounds. Proinflammatory mediators have been shown to up-regulate the enzyme indoleamine 2,3 dioxygenase (IDO) resulting in the accelerated degredation of serotonin precursor …
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An Investigation into Clinically Relevant Determinants of Azole Resistance in Candida albicans
… changes are known to be mediated through gain-of-function mutations in the genes of a fungal-specific transcription factor family known as the zinc cluster transcription factors. Furthermore, genetic changes in the ergosterol biosynthesis genes, ERG11 and ERG3, encoding a C-5 sterol desaturase, …
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Purification and characterization of glutathione reductase isozymes specific for the state of cold hardiness of red spruce (Picea rubens sarg.)
… with respect to temperature adaptation of enzyme function in a species that experiences extreme temperature differences during its life cycle.
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Functional discovery in the oxidative D-galacturonate assimilation pathway and development of the enzyme similarity web tool
… something must be done about the way the functions of those sequences are being discovered and identified. One approach to addressing the problem of the growing number of sequences without a known function is that set forth by the Enzyme Function Initiative (EFI). The goal of the EFI is to …
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Sparse Kernel feature extraction
… feature selection in one view, is applied to an enzyme function prediction case study.
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Comparative Analysis and Speciation of Sulfur K, L-edge X-ray Absorption Spectroscopy
… of life as a key component of protein and enzyme function. It also plays a significant role in the contemporary demand for energy resources in modern society, with associated industrial and commercial applications. Sulfur composes a complex variety of organic and inorganic compounds and are …
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Conformational Entropy in PEPCK Catalysis: Dynamic Motions Critical to Function
Enzymes increase the rate at which chemical reactions occur. How they achieve this rate increase has been an area of intense research for many years. Multiple studies have shown that dynamic motions of individual protein segments can play a role in enzyme function. Our enzyme of interest, …
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