Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Enzyme Evolution"”.
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Study of Enzyme Evolution Within the MLE Subgroup Focusing on Characterizing Member Enzymes for the Purpose of Discovering Relationships Among Sequence, Structure, and Function
… sequence/structure/function relationships of enzymes by elucidating interesting insights into the structural basis for catalysis as well as potential design principles that can be used to develop catalysts for new reactions. The enolase superfamily possesses the most ubiquitous protein fold in …
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Towards The Development Of Intrinsically Fluorescent Unnatural Amino Acids For In Vivo Incorporation Into Proteins
… the synthetase and Acd that can guide our future enzyme evolution strategy.
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Investigation of the Evolutionary Aspects of Thiamin Diphosphate-Dependent Decarboxylases
Thiamin diphosphate (ThDP)-dependent enzymes catalyze a wide range of reactions including the oxidative and nonoxidative decarboxylation of 2-keto acids, carboligation reactions, the cleavage of C-C bonds, and the formation of C-S, C-N, and C-O bonds. Surprisingly, given this diversity, all …
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Evolvability of a viral protease: experimental evolution of catalysis, robustness and specificity
… thesis is to investigate aspects of molecular evolution and enzyme engineering using the experimental evolution of Tobacco Etch Virus cysteine protease (TEV) as a model. I map key features of the local fitness landscape and characterise how they affect details of enzyme evolution. In order to …
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Characterization of Ortho-Succinylbenzoate Synthase (Osbs): A Study of Mechanism, Proficiency and Evolutionary Diversity
"ortho-Succinylbenzoate synthase (OSBS), an enzyme of the enolase superfamily, catalyzes a dehydration reaction in the menaquinone biosynthetic pathway. Menaquinone is an electron acceptor that is utilized in bacteria, archaebacteria and red green algae. OSBSs from over 70 organisms have been …
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MANET and the evolution, structure and function of biological networks
U of I Only Restriction Lifted for Item 50608 on 2016-09-22T20:59:19Z.
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Statistical and Relational Learning for Understanding Enzyme Function
… in living systems is a challenging task. Enzymes are involved in almost all of the chemical processes taking place within the cell. They accelerate chemical reactions by forming a complex with the substrate and therefore lowering the reaction activation energy. The characterisation of the …
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MOLECULAR CHARACTERIZATION AND EXPRESSION OF GENTISATE 1,2-DIOXYGENASE FROM PSEUDOMONAS ALCALIGENES NCIB 9867
… central core resulted in the complete loss of enzyme activity whereas mutations in the flanking regions yielded GDOs with enzyme activities that were reduced by up to 78%. Site directed mutagenesis was also performed on a pair of highly conserved HRH and HXH motifs found within motifs 1 and 2 …
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Evidence for a third thermal parameter of enzymes
… describing the effect of temperature on enzyme activity. Traditionally, the dependence of enzyme activity on temperature has been described by a model (the 'Classical Model') consisting of two processes: the catalytic reaction defined by ΔG‡cat, and irreversible inactivation defined by …
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High-throughput protein engineering tools for efficient exploration and quantitative mapping of protein fitness landscapes
Over the past few decades, directed evolution, a process involving iterative genotype diversification and isolation of desired phenotypes through screening or selection, has had a profound impact on society. It has led to the development of key pharmaceuticals, precision gene editing tools that …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… investigations into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, specificity and …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… investigations into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, specificity and …
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Functional and physiological discovery in the mannonate dehydratase subgroup of the enolase superfamily
… characterization being developed by the Enzyme Function Initiative (EFI). Prior to this study, one member of the ManD subgroup had been characterized and was shown to dehydrate D-mannonate to 2-keto-3-deoxy-D-gluconate. Forty-two additional members of the ManD, selected from across the …
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The Origins of Terpene Infochemicals in Insects: Identification and Evolutionary Analysis of Terpene Synthases in Diverse Lineages
… produced by the action of terpene synthase (TPS) enzymes. While insects lack TPS enzymes found in plants and microbes, there is growing evidence that insect TPS proteins have evolved independently from isoprenyl diphosphate synthase (IDS) enzymes in core terpene metabolism. To gain deeper insight …