Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Enzyme Engineering"”.
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COMPUTATIONAL ENZYME ENGINEERING: FROM MOLECULAR MODELING TO MACHINE LEARNING
… of biocatalysis in modern life sciences. Enzymes, as highly selective and efficient natural catalysts, offer significant advantages for environmentally benign and carbon-neutral processes. However, their practical application is often limited by narrow substrate scope, insufficient …
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Rationalising and accelerating enzyme engineering by quantitative sequence-function profiling in microdroplets
Enzymes catalyse nearly all reactions in Nature's vast chemical repertoire with high precision and under sustainable conditions. To apply them in cost-effective and sustainable biocatalytic production processes in chemical industry, enzymes first need to be modified or "engineered" to work with …
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Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… the native cellular context. A wide panoply of enzyme-mediated labeling methodologies have been developed that couple the power of enzyme specificity with the functional diversity of small molecules to specifically tag proteins within the crowded heterogeneous intracellular environment for a …
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ENZYME ENGINEERING AND GREEN SYNTHESIS OF N-ACYL GLYCINE BIOSURFACTANTS FROM RENEWABLE RAW MATERIALS
… synthesize N-acyl glycines. We then engineer an enzyme (proRML) by reshaping its catalytic pocket to enhance its aminolysis activity and catalytic efficiency by 103-fold and 465-fold, respectively. The evolved proRML (D156S/L258K/L267N/S83D/L58K/R86K/W88V) catalyzed the amidation of a fatty acid …
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Artificial intelligence and protein engineering : information theoretical approaches to modeling enzymatic catalysis
… literature. Despite this, the vast majority of enzyme engineering approaches are based on the energetic optimization of static molecular structures. Using computational sampling techniques, especially rare-event sampling methods like Transition Path Sampling, we can now generate large datasets …
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Evolvability of a viral protease: experimental evolution of catalysis, robustness and specificity
… investigate aspects of molecular evolution and enzyme engineering using the experimental evolution of Tobacco Etch Virus cysteine protease (TEV) as a model. I map key features of the local fitness landscape and characterise how they affect details of enzyme evolution. In order to investigate the …
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Modifying Tryptophan and Indole-Derived Compounds Using Engineered Indole Prenyltransferase Enzymes
<p>Indole Prenyltransferase (IPT) enzymes are present in many microorganisms. They catalyze the transfer of prenyl moieties from natural pyrophosphate donors to tryptophan and other indole-derived small molecules. Prenylation alters the structure of small molecules, enhances their hydrophobicity, …
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FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES
… each focusing on process intensifica:on, enzyme engineering, and biocatalyst immobiliza:on to maximize efficiency, scalability, and environmental sustainability. First, a co-immobilized mul:-enzyme system combining a commercial α-rhamnosidase and an extremophilic β-glycosidase was …
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Plant aromatic amino acid decarboxylases: Evolutionary divergence, physiological function, structure function relationships and biochemical properties
… (AAADs) are a group of economically important enzymes categorically joined through their pyridoxal 5'-phosphate (PLP) dependence and sequence homology. Extensive evolutionary divergence of this enzyme family has resulted in a selection of enzymes with stringent aromatic amino acid substrate …
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Computational Investigation of the Catalytic and Structural Roles of Metals in Metalloenzymes
Metalloenzymes capitalize on the unique roles of metal co-factors and protein scaffolds in catalyzing crucial chemical transformations at ambient conditions with exquisite selectivity. Some metalloenzymes exploit the redox properties of metal cofactors to catalyze challenging reactions, while …
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Raman Chemometrics and Application to Enzyme Kinetics and Urinalysis
… in novel areas. Fast and accurate evaluation of enzyme specific activity is required for engineering enzymes, and results of Raman assays, evaluated in the RDA Toolbox, are successfully correlated to absorbance activity assays of an enzyme WT and mutant library. Further development of this …
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Mechanisms of Flavin-Dependent Monooxygenases Involved in Natural Product Chemistry
… deaths in 2019 alone. One approach is utilizing enzymes to synthesize existing natural products to improve the yields and stereospecificity issue. This dissertation is focused on the biochemical characterization of three enzymes-ZvFMO, OxaD, and CreE-that are implicated in the detoxification of …
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Developing assays to study and screen engineered Escherichia coli NADPH-dependent sulfite reductase
… (e.g., oxidoreductases) through protein engineering. However, successful enzyme engineering studies depend on appropriate assays to evaluate and characterize the desired variants. The Escherichia coli NADPH-dependent sulfite reductase is a heterododecameric complex that mediates one of …
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Biochemical Analysis of Poly(ethylene terephthalate) Film Degradation Kinetics of Engineered IsPETase Variants
… not be directly applicable to kinetics of these enzymes, as the catalysis is occurring under a heterogeneous phase. To improve the fundamental understanding of the enzymatic reaction on the surface and derive an appropriate biochemical model for kinetic analysis, this thesis aims to develop a …
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Using myoglobin models of oxidases for mechanistic understanding of the oxygen reduction reaction
… of the oxygen reduction reaction in metalloenzymes that have remained elusive. There are many pathways for the oxygen reduction reaction to take place in metalloenzymes like oxidases, but strangely, there is a high degree of variance in the active site of these enzymes. Studying the native …
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Bioprospecting novel hemicellulases from the gut microbiota of the common black slug (Arion ater)
… has greatly expanded the repertoire of novel enzymes with putative carbohydrate-active activities able to degrade LCB. Of particular interest, metagenomic exploration of gut microbiota from invertebrates has yielded a significant resource. In this study, the output from the metagenomic …
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Catalytic promiscuity of two plant P450 enzymes: CYP725A4 from Taxus cuspidata and CYP71B102 from Isatis Tinctoria
… mutating key residues at the active site of the enzyme. A mutant V3741 increased the production of T5α-ol by ~10 fold, although it still supported the formation of OCT and iso-OCT as major products. The levels of these compounds in V374I were almost 45% less than in native CYP725A4. Site-directed …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, halogens, and other moieties to endow biological functions and provide their producing organisms an evolutionary advantage. This thesis focuses on mechanistic and functional studies of such …
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