Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 46 for “"Enzymatic digestion"”.
-
In vitro enzymatic digestion of milk proteins
Thesis (M.S.)--Michigan State University. Dept. of Food Science, 1975
-
Molecular interactions of plant cell wall polymers
… to the recalcitrance of lignocellulose to enzymatic digestion. In this thesis the molecular basis of this change in recalcitrance was investigated. The gux1 gux2 mutant, which lacks glucuronic acid in secondary cell walls, was found to be more accessible to hydrolytic enzymes, likely due to …
-
A nucleic acid-based bacterial message export system for cell-to-cell communication
… due to their labile nature and proclivity for enzymatic digestion. Eliminating these obstacles will allow for a larger array of messages to be sent with minimal cellular machinery. Exploiting the bacterial twin-arginine translocation (TAT) pathway and a nucleic-acid binding protein sourced from …
-
Development and Application of a High-Throughput Assay for Discovery of Starch Hydrolase Inhibitors
… the decrease in turbidity over time during the enzymatic digestion of starch. By applying HTS, extracts of wild buckwheat root exhibited strong starch hydrolase inhibitory activity, and diboside A was isolated as a selective inhibitor of pancreatic a-amylase and sucrase. Moreover, …
-
Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… surface biotinylations at low temperature (4°C), enzymatic digestion of the intact fungal cell surface proteins ("whole cell shaving"), biotin-avidin affinity enrichment of biotinylated peptides, liquid chromatography mass spectrometry (LC-MS) based proteomic approach were employed for unambiguous …
-
Chemical synthesis of DNA and RNA containing thio-substituted bases and their post-synthetic modifications
… were validated by various methods (UV, HPLC, enzymatic digestion). Pioneer work in utilization of the versatile sulphur group for post-synthetic modification was also tested. Post-synthetic modification was also carried out on DNA with 6- deoxythioguanosine. Electrophilic reagents with various …
-
Perivascular cell contribution to skeletal muscle repair following physiological injury
… adult male wild type mouse skeletal muscle via enzymatic digestion and FACS. Following 12 days of recovery in culture, isolated pericytes were subjected to strain (10%, 1 Hz) for 1 hr using a Flexercell Strain unit. Conditioned media (CM) was collected from strained (CM-S) or unstrained (CM-NS) …
-
Arsenic Speciation in Food
… to more toxic species was observed when an enzymatic digestion procedure, based on the action of trypsin, was applied to fish except in the case of one of the plaice specimens for which DMAA was characterised in the digest at the mg kg-1 level. Ten volunteers participated in a dietary trial …
-
Developing cultivated mollusks through establishing primary cell culture methods of Eastern Oyster, Crassostrea virginica, as a model bivalve
… were obtained via tissue explant, mechanical and enzymatic digestion. The cells were routinely monitored using an inverted microscope for phase-contrast and fluorescence imaging. Culture vessels were coated with surface proteins such as fibronectin, laminin, matrigel, and poly-d-lysine to promote …
-
Mesophilic fermentative hydrogen production from sewage biosolids
… biosolids which had undergone heat treatment and enzymatic digestion. When fermentation took place at pH 5.5 a peak hydrogen production rate of 3.75 cm<sup>3</sup> min<sup>-1</sup> was observed. At this pH the hydrogen yield was 0.37 mol H<sub>2</sub> mol<sup>-1</sup> carbohydrate, equivalent to …
-
Engineering a Photo-Control Mechanism for RNA Interference
… these effectors are resistant to sugar-specific enzymatic digestion. 1-(4,5-dimethoxy-2-nitrophenyl)diazoethane (DMNPE) was used to cage NA oligonucleotides, including FNAs for controlling RNAi. Photo-control over RNAi was demonstrated in cell culture and developing zebrafish embryos. Caging also …
-
Analysis of glycans and glycoproteins by mass spectrometry
… after subjecting the glycoprotein to enzymatic digestion. The first approach is widely used as a method of getting structural information. In this approach, glycans are usually derivatized using reductive amination to facilitate detection. An improved reductive amination procedure is …
-
Gangliosides와 oxidized low density lipoprotein (oxLDL) 에 의한 신경교세포의 활성조절 및 그 기전에 대한 연구
… analysis 15 F. Determination of NO release 15 G. Enzymatic digestion of sialic acid 16 H. Oxidation of LDL 16 I. Relative electrophoretic mobility (REM) assay 16 J. Thiobarbituric acid reacting substances (TBARS) assay 17 K. Lipid hydroperoxide (LPO) assay 17 L. Enzyme-linked immunosorbent assay …
-
Synthetic enzymatic pathway conversion of cellulosic biomass to hydrogen
… achieve. In order to increase susceptibility to enzymatic digestion, thermochemical pretreatments are applied, with the goals of removing of lignin, the simplest example being soaking in aqueous ammonia (SAA); hemicellulose removal, most often using dilute acid (DA); and increasing cellulose …
-
Characterization of the major extrapallial fluid protein of the mollusc, Mytilus edulis: Implications for function
… phosphorylation) analysis of the EP protein by enzymatic digestion and MALDI-TOF mass spectrometry suggests that protein phosphorylation is not the major source of the charge heterogeneity on the EP protein. Also, the N-linked glycan of the EP protein is proposed to possess one or more …
-
The Role of Perineuronal Nets in Regulating Barrel Cortex Physiology
… in fast spiking (FS) inhibitory interneurons; 3) enzymatic digestion of PNNs only mildly impac spike frequency and firing probability, but significantly reduces input resistance, and spike amplitude in FS inhibitory interneurons and 4) Perturbation of PNNs reduces the temporal range of spontaneous …
-
The role of extracellular matrix in modulation of myocyte Ca2+ signalling and contraction in the isolated rat heart
… be lost during the standard cell isolation by enzymatic process. The aim of this thesis was to investigate the contribution of ECM in myocyte Ca2+ handling in cardiac trabeculae. To achieve this, measurements of isometric force and intracellular Ca2+ (340/380 ratio) in electrically stimulated …
-
Ultrastructure and nanomechanical properties of aggrecan from native cartilage and engineered tissue
… compared to that of newborn aggrecan. After enzymatic digestion of chondroitin sulfate (CS) GAGs, keratan sulfate GAG chains were visualized near the N-terminal domain of a less extended core protein. Direct visualization of aggrecan aggregates confirmed the structure of the constituent …
-
Bioinformatic methods for the analysis of genetic variability and regulation
… Alternative ChIP-seq protocols employing enzymatic digestion of DNA can overcome this limitation by producing mono-nucleosomal footprints of histone PTM. Still, care has to be taken to avoid co-founding effects stemming from the relation between nucleosomes co-localization levels and …
-
Investigation of the Efficacy of Various Proteinase-Treated "Serpula (Treponema) Hyodysenteriae" Vaccines
… "S. hyodysenteriae" culture were subjected to enzymatic digestion of the following proteinases: Proteinase K, proteinase type X-15, proteinase type 2 fungal, papain proteinase, and pepsin proteinase. Sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE) was performed on all bulk …
Page 1 of 3