Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Enzymatic amplification"”.
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Improving multiplexed RNA detection assays by interfacing enzymatic amplification strategies with silicon photonic microring resonators
… these goals by developing assays that combing enzymatic processing steps with silicon photonic microring resonators, a technology pioneered by the Bailey Research Laboratory. While other efforts in lab have been geared to other classes of biomolecules, the developed assays discussed in this …
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Hydrogel-based microfluidic assays for multiplexed medical diagnostics
… to commercial standards without needing amplification. In particular, aptamer-based protein detection using gel particles was found to provide up to three orders of magnitude better sensitivity than competing platforms using the hydrated and flexible gel particles also without using …
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Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… Enzyme-Linked Immunosorbent Assay (ELISA) and enzymatic signal amplification. Specific areas of glass slide beneath the detection area of the chip coated with anti-CD81 antibodies are used to capture EVs. A cocktail mixture of biotin-labeled anti-CD9 and anti-CD63 antibodies were used as …
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Increased sensitivity for lateral flow immunoassays through signal amplification methods
… and silver enhancement solutions, (2) enzymatic creation and precipitation of visible dyes, and (3) nucleic acid amplification test (NAAT)-based signal enhancement. Using the enzymatic amplification-based detection, we developed a prototype of a point-of-care (POC) device including a …
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Paper-based Molecular Technologies for Faster, More Accessible Infectious Disease Diagnostics
… Colorimetric detection was performed either via enzymatic amplification or eosin photopolymerization amplification, a visible light-initiated radical polymerization method for colorimetric signal amplification in diagnostic assays. To better understand the eosin photopolymerization reaction …
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Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… acid self-assembly, all without requiring enzymatic amplification or fluorescence labelling. To establish a fundamental understanding of signal generation mechanisms, I first conducted a systematic investigation into the relationship between nanopore current signals and molecular …
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Application of polymerization-based amplification in point-of-care diagnostics
… settings. Existing colorimetric methods based on enzymatic reactions and gold nanoparticles often produce results that must be read within a specified time interval to ensure their validity. In many instances, a user has to wait several minutes for the color to develop. Moreover, the result can be …
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Development of silicon photonic microring resonator biosensors for multiplexed cytokine assays and in vitro diagnostics
… of nanoparticles and sub-micron beads for signal amplification are described in Chapter 7, also demonstrating the ability to monitor single bead binding events. Chapter 8 describes an alternative cytokine signal enhancement strategy based on enzymatic amplification for human cerebrospinal fluid …