Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 59 for “"Enzymatic Reactions"”.
-
Mass Spectrometric Studies of Enzymatic Reactions
… mass spectrometry for the study of proteolytic enzymatic reactions was evaluated. Two types of soft ionization were employed, electrohydrodynamic ionization and fast atom bombardment.
-
Computational Studies Of Enzymatic Reactions And Inorganic Compounds
… results in studying a variety of chemical reactions. However, the current computing resources limits its use in large-size systems such as enzymes. Compared to QM, Molecular Mechanics (MM) have an advantage in computing time but disadvantage in accuracy, and MM cannot be used in reactions …
-
Enzymatic reactions involving glycine within the central nervous system of the rat
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
-
Hydrodynamics and Diffusion of Membrane Proteins to Engineer Chemical Reactions in Synthetic Biomembrane Platforms
… platforms for use in mimicking and engineering enzymatic reactions requires progress in a few different areas. These areas include an understanding of transport phenomena in lipid bilayers, the incorporation of transmembrane proteins into SLBs, the fluidization of those transmembrane proteins, …
-
Nature's way: the effects of hydrogen bond networks on acidities, catalysis, and molecular recognition
… multiple hydrogen bonds to better understand enzymatic reactions. Anion recognition abilities of synthetic hydrogen bonded receptors are studied to probe the role of hydrogen bonds in anion channels. At the same time, environmentally friendly green acids are developed and their catalytic …
-
Molecular communication between artificial cells
… compartments in water solutions and to carry out enzymatic reactions or gene expression. These artificial cells are able to establish communication pathways with natural cells and may be further developed to fight pathogens or cancer cells, for example. Despite these promising results, …
-
Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System
… of diffusion or reaction. We also found that enzymatic reactions in nanochannels were very effective due to short diffusion length and high surface area to volume ratio. A bead based ELISA was developed to exploit the rapid binding reactions in the bulk and efficient enzymatic conversion in …
-
Changes in Mammalian Chromatin Structure as a Function of Protein-Poly(ADP-Ribosyl)ation by Endonuclease Digestion
… and enzyme concentration. Endonuclease enzymatic reactions were stopped with EDTA. Samples were run in 1.5% agarose gels and the oligonucleosomal electrophoretic migration patterns compared. Endonuclease experiments were carried out with rat liver chromatin pre-incubated in the presence …
-
Cytometry of Reaction Rate Constant (Crrc): Optimization of the Technique and Development of a Novel Protocol to Assess Cell Population Heterogeneity Based on Aldh1a1 Activity in Vitro
… cellular heterogeneity based on the activity of enzymatic reactions. In the past, CRRC was able to assess cellular heterogeneity in vitro by investigating the activity of ABC-transporter enzymes. However, CRRC showed poor robustness to highly motile cells. Here, I report on the development of a …
-
Metals in Chemistry and Biology: Computational Chemistry Studies
Numerous enzymatic reactions are controlled by the chemistry of metallic ions. This dissertation investigates the electronic properties of three transition metal (copper, chromium, and nickel) complexes and describes modeling studies performed on glutathione synthetase. (1) Copper nitrene complexes …
-
Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli
… reducing equivalent NADPH is required in several enzymatic reactions leading up to the synthesis of high-value compounds like polymers, chiral alcohols, and antibiotics. However, it’s a highly costly compound with limited intracellular availability. This study focuses on the genetic manipulation …
-
Biological detection by means of mass reduction in a suspended microchannel resonator
… researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution.
-
Micromachined Electrochemical Sensors For Hydrogen Peroxide And Chlorine Detection
… and wastewater treatment and biochemical enzymatic reactions is critical. Given the gamut of areas that hydrogen peroxide is a key component of; its detection assumes great importance. Similarly chlorine has long been used as a disinfectant for making drinking water safe, but excessive …
-
Touch-and-go chemistry: development of materials for and demonstration of a contact-initiated polymerization
… come into proximity in the correct orientation. Enzymatic reactions display improved reaction rates because catalytic groups are prearranged in proximity to the substrate. It follows that one can control a reaction rate by controlling the spatial proximity of two reactive groups. To demonstrate …
-
Modifying Tryptophan and Indole-Derived Compounds Using Engineered Indole Prenyltransferase Enzymes
… enzymes were purified and screened. Our in vitro enzymatic reactions, coupled with HPLC-MS, kinetic data, in addition to 1- and 2D nuclear magnetic resonance spectroscopy, show that the three mutants are capable of catalyzing diprenylation reactions. This work highlights the crucial role of enzyme …
-
Towards Deep Learning Models of Metabolism
… play a critical role in catalyzing the chemical reactions that underpin metabolic processes in living organisms. Despite their importance, a vast majority of enzymes remain uncharacterized, limiting our understanding of their potential roles in metabolism and disease. This thesis aims to address …
-
Sideroflexins enable mitochondrial transport of polar neutral amino acids
… in eukaryotic cells, facilitating diverse enzymatic reactions that support cell function. However, this compartmentalization of metabolism necessitates regulated transport of metabolites across the inner mitochondrial membrane. While many proteins enabling mitochondrial membrane transport …
-
Modification of Fermentation by Exogenous Energy Input
… production. Light schemes on the scale of enzymatic reactions, yeast generation times, and circadian rhythms produced the most ethanol. Discrete blocks and duration of illumination were used to elucidate where light had the most influence over yeast metabolism and fermentation. Late lag …
-
Mechanisms of biochemical reactions within crowded environments
… and protein folding. Thus, biochemical reactions that take place in such crowded conditions differ from diluted test tube conditions, and a considerable effort has been invested in order to understand such differences. In this work, we combine different computationally tools to …
Page 1 of 3