Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Environmental Isolates"”.
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Isolation and characterization of plasmids from human and environmental isolates of mycobacteria
Human clinical (n=131) and environmental (n=226) isolates of the Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) complex were screened for plasmids in an effort to increase knowledge about the genetics and epidemiology of these pathogenic bacteria. Approximately 50% of the clinical …
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Characterizing the Persistence of Sewage Enterococci in Mississippi Coastal Waters
… persistence have been done but few under natural environmental conditions. The purpose of this study was to investigate how long sewage enterococci and enterococcal DNA persist in beach water and to determine whether there is a difference in the genetic diversity and hardiness of sewage vs. …
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Gliotoxin Production in Aspergillus fumigatus
… amounts of gliotoxin produced from clinical and environmental isolates of A. fumigatus. We hypothesized that the amount of gliotoxin produced would be higher in the clinical isolates in comparison to the environmental isolates. Cell assay results showed that the clinical isolates produced more …
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The impact of bile and anaerobic stress on Listeria monocytogenes isolates
… by anaerobic conditions. In total 154 food/environmental isolates and 74 clinical isolates were screened. A bile tolerance assay and a BSH enzymatic agar plate assay were developed. Experiments were carried out at a lowered pH and/or anaerobic conditions to simulate conditions in the human …
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Screening For Cry Genes From Bacillus Thuringiensis In Various Textural Classes Of Southwest Missouri Soils
… in a variety of environments, primarily in soil. Environmental strains of B. thuringiensis were isolated from 21 soil samples and each soil sample was classified texturally based on percentage of sand, silt, and clay. Population densities were used to analyze the frequency of B. thuringiensis and …
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Evaluation of Dunaliella isolates from Sua Pan (Botswana) solar salt evaporation ponds for production of ß-carotene
… The study assessed the productivity of the environmental isolates by means of growth and carotenoid biosynthesis properties at indoor laboratory scale...
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Environmental and biological drivers of bacterial and antimicrobial resistance carriage by house flies (Musca domestica L.)
… of this study was to assess biological and environmental drivers of bacterial abundance, as well as trends of AMR for suspected coliform (SC) bacteria carried by adult house flies in both dairy and beef cattle production facilities in Kansas. Cultured aerobic bacteria and SC were enumerated …
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Monoclonal and phage-displayed antibodies as lipopolysaccharide serotyping reagents and their use in typing colony variants of Vibrio vulnificus
… that can be typed. Analysis of V. vulnificus isolates from the United States, Europe, and Asia demonstrated that the O1/O5 serovar is dominate in clinical isolates, serovars O3 and O4 are dominate in the environmental isolates of Europe, and that most environmental isolates from United States …
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Linking sequence to function in microbial genomics
… project, I completed whole-genome sequencing of environmental isolates derived from single, cultivable cells and employ mutational and horizontal transfer analysis to demonstrate adaptation to harsh environmental conditions in contaminated groundwater. These projects demonstrate the rich …
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Host cell death in Legionella pneumophila pathogenesis and immunity
… macrophages respond to <em>Legionella </em>environmental isolates, which are responsible for most of the Legionnaires' disease outbreaks so far, we analyzed the intracellular growth of a few such isolates in A/J mouse macrophages, which is permissive to most, if not all, laboratory strains. …
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Epidemiology of <i>Mycobacterium avium</i> complex infecting AIDS patients
… we attempted to recover M. avium complex from environmental samples in geographical areas (Boston, Massachusetts; Hanover, New Hampshire; Helsinki, Finland; Nairobi, Kenya; and Kinsasha, Zaire) located near <i>M. avium</i> infected AIDS patients. Although <i>M. avium</i> was recovered from …
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A comparative and phylogenetic analysis of the genome of Pseudomonas aeruginosa
… a LC-MS metabolomics analysis of the CF isolates grown in artificial sputum media to mimic chronic lung infection. Further to this, in an attempt at mapping the core genome of P. aeruginosa and providing the community with a greater understanding of the genomic basis of this pathogen, we …
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Evolution and Regulation of Virulence and Antimicrobial Resistance in Pseudomonas aeruginosa
… that those are prevalent amongst clinical and environmental isolates and not restricted to a particular region of the gene. My findings describe a novel mechanism by which P. aeruginosa can restore virulence in an SRindependent manner and show that mexT can be a mutational hotspot for the …
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Environmental proteome profiling applied to the study of polybacterial metal resistance and adaptation
Environmental biotechnology can be defined as the use of biotechnology to solve environmental engineering problems, frequently involving bacterial communities and unsequenced species. Here we define environmental proteomics as the proteomic profiling of microorganisms of environmental relevance, …
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Isolation, identification and characterisation of novel actinobacteria from Zambian hot-springs
… the search for a novel hydantoinase from environmental isolates obtained from two Zambian hot-springs. The aim of this study was to isolate, characterise and screen novel actinobacteria for industrially relevant enzymes including hydantoinases. Fifty one actinobacteria were isolated. …
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Investigation of the distribution of motile Aeromonas within Grampian water supplies.
… suitability of Aerokey 2 for identifying water isolates to species level found that this scheme, designed originally for clinical isolates and not tested with drinking water isolates to date, was incapable of reliably speciating such environmental isolates. Chlorine resistance of Aeromonas was …
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Detection and survival of Escherichia coli O157:H7 in cattle water troughs and the effects of cetylpyridinium chloride against Escherichia coli O157:H7 biofilms on the surface of stainless steel
… the growth and survival of human E. coli O157:H7 isolates was significantly lower than environmental isolates at lower temperatures (5°C and 15°C). The biofilm study showed that <1.0% CPC was effective in inactivation of E. coli O157:H7 biofilms grown on stainless steel in all treatment conditions.
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Studies of new natural products and their biosynthesis spearheaded by high-resolution mass spectrometry
… PrISM as a full discovery platform applied to environmental isolates of the genera Bacillus and Streptomyces, where new natural products and undiscovered biosynthetic gene clusters were characterized.
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Implementation of molecular techniques in the diagnosis of legionnaires’ disease and in the investigation of legionella outbreaks
… consequently typing of both clinical and environmental isolates is crucial to rapidly identify the possible source and prevent further cases. Legionellae are difficult to isolate by culture, moreover as respiratory samples are not available for up to 65% patients, alternative techniques …
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Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries
… A diverse bacterial isolate library (N= 1,866 isolates) was constructed by employing a variety of culturing techniques across thirty-four of the fermentation communities selected based on superior performance in the biofuel platform. Phylogenetic analysis of partial 16S rDNA sequences placed …
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