Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Envelope Stress"”.
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Further characterisation of the envelope stress responses of Salmonella Typhimurium
… Salmonella spp. to sense and adapt to exogenous stresses contributes towards infection severity and prevalence. The envelope stress responses promote survival within and between hosts by maintaining envelope homeostasis and promoting the rapid response to environmental insults. In this study, we …
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Intramembrane signal transduction and cell envelope stress response in <i>Bacillus subtilis</i>
… für ein Bakterium lebenswichtig. Die Zellhüllstress-Antwort in Bacillus subtilis wird durch sieben signaltransduzierende Systeme vermittelt, zu denen drei ECF-sigma-Faktoren und vier Zweikomponentensysteme gehören. Eines dieser Zweikomponentensysteme ist das LiaRS-System, welches auf …
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Outer Membrane Vesicle Production in Escherichia coli Relieves Envelope Stress and is Modulated by Changes in Peptidoglycan
… with a minor role in methionine transport) and envelope stress (ΔdegP, dual function periplasmic chaperone/ protease responsible for managing proteinaceous waste). This research showed that the growth defect of ΔnlpAΔdegP correlated with reduced OMV production with respect to the …
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Influence of Membrane-Damaging Agents and the Sigma Factor AlgU on the Induction of the MexCD-OprJ Efflux System of Pseudomonas Aeruginosa
… P. aeruginosa sigma factor AlgU is responsive to envelope stress, it was of interest to ascertain whether AlgU is capable of mediating this increased mexCD-oprJ expression. Thus, the impact of AlgU loss on mexCD-oprJ expression in response to membrane-damaging agents was assessed in a algU …
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PEPTIDOGLYCAN REMODELLING FACTORS: KEY PLAYERS IN CELL DIVISION AND HOMEOSTASIS MAINTENANCE OF ESCHERICHIA COLI
… integrity and coordinated growth of the cell envelope are crucial for survival, especially under environmental stress conditions. The lipopolysaccharide-containing outer membrane (OM) acts as a selective barrier against toxic molecules and provides additional mechanical support that …
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Investigating Hfq-dependent Small Regulatory RNA, Bsr34, and Its Roles in Brucella abortus Physiology and Pathogenicity
… regulators of bacterial virulence and stress adaptation, yet remain largely uncharacterized in Brucella. Following the identification of Hfq-associated sRNA candidates in B. suis, this study provides the first functional characterization of Bsr34, an Hfq-dependent sRNA in B. abortus …
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A role for a predicted E. coli inner membrane lipoprotein in cell envelope integrity
… is the possession of a double-membraned cell envelope. The outer membrane provides an additional layer of protection against hydrophobic molecules including antibiotics, contributing to the multidrug resistance crisis. The Gram-negative membrane is composed of phospholipids, glycolipids, and …
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Understanding the Genome-Wide Response of Streptomyces coelicolor to the Glycopeptide Antibiotic Teicoplanin
… that ΔvanJ exhibited increased signs of cellular stress that were attributed to a delayed induction of genes involved in the osmotic, redox, and cell envelope stress responses. This dataset led to the functional characterisation of a group of genes with phosphatidic acid phosphatase activity which …
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Extracytoplasmic stress response systems in S. Typhimurium
… gene expression following activation of certain stress response systems. In gram negative bacteria such as Salmonella, envelope stress responses (ESR) are response systems that target stresses affecting components of the cell envelope such as the periplasm and outer membrane proteins. The two …
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Characterization of NfxB and PA4596, Two Repressors of the mexCD-oprJ Operon Encoding an RND-Type Multidrug Efflux Pump in Pseudomonas aeruginosa
… found to be induced in response to CHX-promoted envelope stress in an AlgU-dependent manner and is directly repressed by NfxB. Loss of PA4596 resulted in increased resistance to the biocide CHX, shown to be a result of increased CHX-dependent expression of mexCD-oprJ. Susceptibility to CHX was …
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Analysis of a bacterial serine/threonine kinase
… of cell surface appendages and is one of three envelope stress response systems, it is hypothesized that RdoA serves to relay Cpx activation signals. This is supported by studies on the effect of pH on Cpx activity in wildtype and rdoA- cells presented here. RdoA homologues are present in at …
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Control of Shigella sonnei and adhesive invasive Escherichia coli infections with a natural product which inhibits the bacterial oxidoreductase DsbA
… that propolin D caused extensive bacterial envelopE stress, as indicated by a changed expression of key bacterial genes involved in stress responses. Propolin D also enhanced the autophagy activity of the host cells; the intracellular growth of S. sonnei was significantly reduced in wild …
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Investigation of Bacillus subtilis Sigma Factor Dynamics Using Improved Single Cell Tools
… factors, which are an important part of the cell envelope stress response, under their specific stresses. To do this we use transcriptional reporters of sigma factors, quantitative single cell snapshots, time-lapse microscopy, and microfluidics.\\ By developing an improved microfluidics setup for …
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Structure and function analysis of the natural product plantazolicin, a bacillus anthracis-specific antibiotic
… upregulation of genes within the cell envelope stress response. Using fluorescence microscopy, PZN localizes to distinct ~200 nm wide foci within the envelope; furthermore, like other cell envelope-acting compounds, PZN depolarizes the B. anthracis membrane. Upon selection and …
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A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli
… key mutations that conferred changes to cell envelope stress responses, morphology, and transcriptional/translational regulation that contributed to its fitness. The final E. coli expression system developed in this thesis ultimately improved the soluble yield of two model ‘difficult to …
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Surface Localization Determinants of Borrelia burgdorferi Lipoproteins
… lipoprotein that influences sorting within the envelope. One facet of this study utilized an N-proximal tandem negative charge (Glu-Asp) that served as an inner membrane retention signal in OspA20:mRFP1 as a target for mutagenesis. A library of random mutants in the two codons was generated and …
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Conformational Rearrangements In The Sensory Rcsf/Omp Complex Mediate Signal Transduction Across The Bacterial Cell Envelope
<p>Timely detection and repair of envelope damage are paramount for bacterial survival. The Regulator of Capsule Synthesis (Rcs) stress response is a complex signaling cascade that monitors gram-negative cell envelope integrity and can transduce the stress signals across the multilayered envelope …