Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Enrichment strategy"”.
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Structural Characterization of Donkey Lactoferrin: Amino Acid Sequence and Glycan Compositions
… by chymotrypsin digestion, TiO2 and HILIC enrichment strategy, reversed-phase high performance liquid chromatography, electrospray mass spectrometry, high collision dissociation fragmentation, is reported. 26 different glycan compositions were identified, linked to the protein backbone via …
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Investigative Application of the Intrinsic Extended Finite Element Method for the Computational Characterization of Composite Materials
… (IXFEM). The IXFEM employs a localized intrinsic enrichment strategy to treat arbitrary discontinuities defined through the level-set method separate from the problem domain discretization, i.e. the finite element (FE) mesh. A global domain decomposition identifies local subdomains for building …
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Study of space-time evolution of flux in a long-life traveling wave reactor
… non-enriched region with fertile material. This enrichment strategy is aimed to allow breed-and-burn in the core. The core, on the outside, is surrounded by reflector of uniform thickness. To facilitate the study of flux profile, the two primary regions in the core are further divided into finer …
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SORTASE AND CHEMICAL BIOLOGY AS TOOLS TO STUDY UBIQUITINATION
… and genetic code expansion to develop a strategy for site-specific modification of proteins of interest with user-defined polyubiquitin chains. We exemplify the broad utility of this strategy for multiple different proteins of interest, including DNA replication protein PCNA. As an …
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Development and Application of Large-Scale Protein Folding Stability Analysis in Drug Target Identification and Disease Biomarker Discovery
… this work is to develop a novel chemo-selection strategy to improve the bottom-up proteomics readout in proteome-wide limited proteolysis experiments. Another goal of this work is to apply these methods to the target identification of two drugs with known mode of action, and to the biomarker …
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
… Therefore, it is important to incorporate enrichment during sample preparation in endogenous protein quantifications. Traditionally, solid phase extraction, protein precipitation, and filter-aided sample preparation could help clean up the target analyte. However, more targeted enrichment …
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The Use of Spectroscopic Techniques in the Characterization of Mycobacterial Metabolites
… low sensitivity, we used <sup>13</sup>C-isotope enrichment strategy to improve the detection threshold of NMR. We developed and investigated the use of three NMR-based profiles. The first is for the cell wall associated lipids, the second is for covalently bound mycolic acids and the third deals …
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Studio in vitro dell'espressione di varianti del gene CFTR con ruolo patofisiologico non ancora definito
… of the possible alternatives, we chose a target enrichment strategy and designed a set of probes covering the full-length CFTR to be used for DNA capture (custom capture kit, Nimblegen SeqCap EZ Library, Roche). The application of NGS will lead to the improvement of time and cost of molecular …
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Application and Evaluation of a Chemical Modification- and Mass Spectrometry-Based Thermodynamic Assay for the Study of Protein-Ligand Interactions in Complex Mixtures
… modification- and mass spectrometry-based strategy for detecting protein-ligand interactions by monitoring the change in thermodynamic stability of proteins upon ligand binding. This is accomplished by monitoring the denaturant dependent oxidation of globally protected methionine residues. …
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A Signature Enrichment Design With Bayesian Adaptive Randomization For Cancer Clinical Trials
… we proposed a cross-validated signature enrichment design combined with Bayesian response-adaptive randomization. We evaluated the performance of this design using four criteria based on the benefits and losses for individuals inside and outside of the clinical trial. The proposed design …