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Showing 1 to 20 of 62 for “"Enolase"”.
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Isolation and Characterization of Enolase and Enolase Isozymes From Chum Salmon (Oncorhynchus Keta)
Made available in DSpace on 2014-12-09T17:35:52Z (GMT). No. of bitstreams: 1 6801860.pdf: 3972606 bytes, checksum: 1c33f94c298e90e93f3947e3acd2b2ff (MD5) Previous issue date: 1967
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Chemical Modification of Yeast Enolase
Made available in DSpace on 2014-12-05T19:37:55Z (GMT). No. of bitstreams: 1 6202885.pdf: 2306341 bytes, checksum: d6b410e0aac6ac71608aa1c2f3c9fea2 (MD5) Previous issue date: 1962
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Isolation and Characterization of Muscle Enolase
Made available in DSpace on 2014-12-05T19:37:41Z (GMT). No. of bitstreams: 1 6101624.pdf: 3653820 bytes, checksum: 903379eee497cbfb8d675aa1be93800e (MD5) Previous issue date: 1961
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Discovery of Function in the Enolase Superfamily
… are incorrectly annotated. We are using the enolase superfamily as a model system to try to solve that problem. To date, >4000 members have been identified in the enolase superfamily, of which ∼50% have unknown functions. The muconate lactonizing enzyme (MLE) subgroup contains a family of …
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Discovery of function in the enolase superfamily
… are incorrectly annotated. We are using the enolase superfamily as a model system to try to solve that problem. To date, >4000 members have been identified in the enolase superfamily, of which ~50% have unknown functions. The muconate lactonizing enzyme (MLE) subgroup contains a family of …
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The effects of sodium cations on rabbit muscle enolase : evidence for the binding of 2PGA to apo-enolase with Na⁺
Enolase, a dimeric enzyme, in the presence of Mg$\sp{2+}$ catalyzes the reversible dehydration of 2-phospho-D-glycerate (2PGA) to form phosphoenolpyruvate (PEP) in the glycolytic pathway. Studies in the inactivation of rabbit muscle enolase by NaClO$\sb4$ show that both Mg$\sp{2+}$ and 2PGA protect …
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Natural and Engineered Promiscuity Within the Enolase Superfamily
The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti …
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Chemical and Physical Studies on Rabbit Muscle Enolase
Made available in DSpace on 2014-12-09T17:35:00Z (GMT). No. of bitstreams: 1 6500936.pdf: 3572498 bytes, checksum: 15de98ae8cccee75855c370fdd0925b6 (MD5) Previous issue date: 1964
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Galactonate Dehydratase: Exploring the Frontiers of the Enolase Superfamily
The crystal structure of GalD indicates that His 185 may serve as a general acid catalyst to facilitate the departure of the 3-OH leaving group and/or stereospecifically protonate the product in the subsequent tautomerization reaction. The function of His 185 was investigated by comparing the …
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The Identification of Novel Enzymatic Functions in the Enolase Superfamily
… the functional and catalytic diversity in the enolase superfamily is a valuable enterprise in accelerating the field of genomic enzymology. This is of paramount importance as the accumulation of genomic data is threatening to outstrip the ability for biochemists to study proteins at a pace that …
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Functional and Mechanistic Investigations of Enzymes in the Enolase Superfamily
In this thesis, the enolase superfamily is traced phylogentically and studied in detail to ascertain which active site residues are necessary to maintain function, kinetic competence, and/or substrate specificity. Methods for understanding the details of many enzymes in the enolase superfamily are …
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Isolation and Characterization of Enolase From Coho Salmon (Oncorhynchus Kisutch)
Made available in DSpace on 2014-12-09T17:35:57Z (GMT). No. of bitstreams: 1 6808209.pdf: 4208564 bytes, checksum: 27c6790a7b0bd147c4d5e526bebd1a52 (MD5) Previous issue date: 1967
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Enolase Dissociation Induced by High Pressure. A Study by Fluorescence Polarization
Enolase is a protein of M.W. 90 kilodaltons, formed by two identical subunits of 45 kilodaltons each. Fluorescence polarization is the main spectroscopic property followed to characterize the dissociation of this enzyme under pressure.
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Isolation and Characterization of Enolase From Rainbow Trout (Salmo Gairdnerii Gairdnerii)
Made available in DSpace on 2014-12-09T17:35:31Z (GMT). No. of bitstreams: 1 6612308.pdf: 2673346 bytes, checksum: f02b5d4abf6c17a8dcd33783edcba03f (MD5) Previous issue date: 1966
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
… superfamily explored in this work is that of the enolase superfamily. Genomic context and primary amino acid sequence make it clear that although the enzymes in this superfamily are structurally and mechanistically related, the chemistry and substrates are varied. Two highly divergent enzymes from …
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Identification of the surface-associate proteins and functions of enolase of streptococcus mutans
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Functional and physiological discovery in the mannonate dehydratase subgroup of the enolase superfamily
… the mannonate dehydratase subgroup (ManD) of the enolase superfamily (ENS). The outcome affirms the dangers of homology-based annotations while discovering novel metabolic pathways. Furthermore, the experimental verification of these pathways (in vitro and in vivo) has provided a platform to test …
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Effect of Photoperiod on Developmental Morphology and Enolase Isoenzyme Immunohistochemistry in Rat and Djungarian Hamster Superficial Pineal Glands
… levels of the glycolytic enzyme neuron-specific enolase (NSE) are thought to reflect increased physiological demands placed on neurons and neuroendocrine tissues. Homodimer non-neuronal enolase isoenzyme (NNE) is immunolocalized to cells, and the hybrid enolase (consisting of subunits from NSE …
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Anti-alpha-enolase Antibody as a Serologic Marker and its Correlation with Disease Severity in Intestinal Behcet's Disease
… Analyzing factors 3 C. Detection of anti-alpha-enolase antibody by ELISA 4 D. Statistical analysis 5 III.RESULTS 6 A. Measurement of serum IgM AAEA and its prevalence in intestinal BD 6 B. Clinical characteristics and laboratory findings of IgM AAEA positive and negative groups 9 C. Comparison …
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