Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Endosomal sorting"”.
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Investigation of early endosomal sorting and budding
… Funktionen von frühen Endosomen. Während endosomale Fusion gut charakterisiert ist, fehlen Kenntnisse über den Prozess der Sortierung. Generell wird von beiden Prozessen angenommen, dass sie von verschiedenen, unabhängigen Mechanismen gesteuert werden. Diese Arbeit stellt einen neuen …
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The interconnected roles of spastin and protrudin in endosomal sorting
Endosomal tubule fission is associated with endoplasmic reticulum (ER)-endosome membrane contact sites and several ER proteins are required for efficient endosomal tubule fission. Our lab has previously identified two hereditary spastic paraplegia (HSP)- associated proteins, spastin (SPG4) and …
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The interrelationship between the endosomal sorting regulators RAB21, Retromer and WASH complex
… diseases (several types of cancer). The cargo sorting occurs in the endosomal network. The early endosome is the first step in the sorting process. The early endosome recruits several sorting complexes namely retromer, commander and WASH complex to facilitate the proper trafficking of cargo. …
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Experimental and modeling studies of endosomal sorting using the epidermal growth factor/receptor system in fibroblasts
… endosomes and how this process affects ligand sorting.
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Endosomal Sorting Complex Required for Transport (ESCRT): Investigation of the expression and functional roles in neurodegenerative diseases
Endosomal Sorting Complex Required for Transport (ESCRT) is responsible for trafficking of misfolded proteins, unwanted organelles and ubiquitinated proteins to be degraded by lysosome. Abnormalities in ESCRT proteins have been linked to various neurodegenerative diseases such as Alzheimer's …
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Characterization of The Ubiquitin Ligase, Ube4B, In Endocytic Trafficking
… modification allows EGFR recognition by endosomal protein complexes that mediate protein inclusion into MVB vesicles from those that remain on the MVB membrane for incorporation into other cellular structures (e.g. plasma membrane, Golgi). The endosomal sorting machinery consists of a …
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Molecular Mechanisms Regulating Chemokine Receptor CXCR4 Signaling and Trafficking
… The ubiquitin moiety on CXCR4 serves as a sorting signal for entry into the endosomal sorting complex required for transport (ESCRT) pathway. This pathway consists of four different protein complexes (ESCRT-0, I, II and III), plus several accessory factors, that act in a sequential and …
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Endocytic Trafficking of The Amyloid Precursor Protein In Rat Cortical Neurons
… and Aβ aggregation, the mechanisms underlying endosomal production of Aβ peptides and their secretion remain unclear. Using endosomes isolated from cultured primary neurons, we determined that the trafficking of APP from the endosomal membrane into internal vesicles of late …
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Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators
Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
<p>The endosomal sorting complexes required for transport (ESCRT) machinery is a ubiquitin-dependent molecular mechanism made of up of four individual complexes: ESCRT-0, -I, -II, III, that is necessary for regulating the degradation of cell surface receptors directed towards the lysosome. Not only …
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Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1
… receptor-mediated endocytosis as well as endosomal sorting for lysosomal degradation. Epsin1 is an endocytic adaptor protein with three tandem UIMs (Ubiquitin Interacting Motifs) which are responsible for the highly specific interaction between epsin and ubiquitylated receptors. Epsin1 is …
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Role And Regulation Of ESCRT-III In Multivesiculr Body Biogenesis
Endosomal sorting complex required for transports: ESCRT) machinery responsible for multivesicular body: MVB) biogenesis is essential for receptor downregulation, viral budding and cytokinesis. ESCRT-III is a large polymer built from related ESCRT-III proteins that is thought to help generate …
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Mechanisms of microparticle release from human platelets
… independent of influx of hydrophilic ions or the endosomal sorting complex required for transport. It was found that microparticles were also released from platelets undergoing apoptosis, which would progress to secondary necrosis in vitro as they were not cleared. Microparticle release in …
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Shedding light on the molecular interactomes of fast, complex biological processes using multispectral imaging with uncompromised spatiotemporal resolution
… complex biological processes, for instance, endosomal receptor sorting. This process occurs within a few seconds and is regulated by tens, or hundreds, of components on the endosomal membrane. Furthermore, these endosomes themselves move on the µm/sec range in 3D within the cell, making this …
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Mechanisms of Multivesicular Body Biogenesis and Exosome Release
… ist dabei nicht abhängig von der ESCRT-(endosomal sorting complex required for transport)-Maschinerie ab, sondern bedarf der Bildung von Ceramiden. Es konnte eine Anreicherung von Ceramiden in Exosomen nachgewiesen werden; gleichzeitig reduzierte die Hemmung von neutralen …
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Analysis of trafficking dynamics and cellular response in the interleukin-2 ligand/receptor system
… of the native and intermediate states under endosomal sorting conditions when compared to wild-type IL-2. This behavior correlates with differential pH-sensitivities of receptor binding affinity measured for IL-2 and the 201 variant. improved sorting leads to an extended half-life of intact …
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The Role of FAM21 in the Regulation of Mitochondrial Dynamics
… complex, recruits WASH and Arp2/3 complexes to endosomal membranes. Coordinated activity of WASH and Arp2/3 complexes drives actin polymerization to facilitate endosomal sorting and potentially fission. To address whether a similar mechanism could be at play in mitochondrial division, the role …
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Regulation of autophagosome formation and maturation by neurodegeneration-associated proteins
… endosomes. Autophagosomes are closed by the endosomal sorting complex required for transport (ESCRT) complex and then released by dynamin 2 (DNM2) before fusing with lysosomes to enable the degradation of the autophagy substrates. Impaired autophagic clearance is a shared hallmark of …
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Structural and biochemical characterisation of pUL7 and pUL51, conserved tegument proteins required for efficient herpesvirus assembly
… adhesions. HSV-1 pUL51 structurally resembles endosomal sorting complex required for transport (ESCRT)-III components, a class of cellular proteins involved in membrane scission. HSV-1 pUL51 binds lipids when recombinantly expressed, and forms filamentous and ring-shaped polymers that resemble …
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Using Chemical Probes to Examine Cellular Activities
… protein that is recruited in anaphase by the endosomal sorting complex required for transport (ESCRT) to the reforming nuclear envelope, where spastin is proposed to sever stable microtubules. However, we currently lack an understanding of spastin's dynamics during anaphase, particularly the …
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