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Showing 1 to 20 of 54 for “"Endonucleases"”.
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Bioinformatic analysis: Retroposon sequences containing multiple putative endonucleases
Among the retroposons, the source of the endonuclease activity is known to be variable. We investigated whether any retroposon genomes encode potential multiple endonuclease functions. Amino acid alignments were generated to reveal the presence of the characterized ordered-series-of-motifs (OSM) …
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Genomic nucleic acid memory storage with directed endonucleases
Technologies for long-term recording of cellular pathway activation are constrained by the difficultly to constantly monitor transient signaling events and expression of target genes. To overcome these limitations we designed a recording tool that uses the transcriptional output of a signaling …
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
LAGLIDADG Homing Endonucleases (LHEs) are a family of highly specific DNA-cutting enzymes capable of recognizing target sequences of ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter …
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Intrinsic Cleavage Inhibition by Thermophilic Type II-S Restriction Endonucleases
<p>Restriction endonucleases (REs) are the cornerstone research tools used in molecular biology and biotechnology. These enzymes are essential to several ground-breaking achievements, including DNA cloning, gene editing, and genome mapping. A subset of these enzymes, Type II-S restriction …
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DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
… Different classes of naturally occurring DNA endonucleases, including homing endonucleases, have therefore been explored as candidates for genome modification studies as they target long stretches of DNA. Homing endonucleases are mobile genetic elements whose biological role is to introduce …
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Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti
… fever, and dengue hemorrhagic fever. Homing endonucleases are selfish genetic elements which introduce double-stranded DNA (dsDNA) breaks in a sequence-specific manner. In this study, we aimed to validate a somatic assay to detect recombinant homing endonuclease (rHE)-induced dsDNA breaks in …
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In Vitro and in Vivo Studies of the Recognition of Double-Strand DNA by EcoRI and RsrI Endonucleases
Made available in DSpace on 2014-12-17T21:58:46Z (GMT). No. of bitstreams: 1 9503183.pdf: 7252488 bytes, checksum: 309c1eef47e382d24902e0bdd38c89ed (MD5) Previous issue date: 1994
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
<p>We have modified the combinatorial selection method Restriction Endonuclease Protection and Selection Assay (REPSA) to work in high temperature conditions for the discovery of new DNA-binding proteins in thermophiles (HT-REPSA). We utilized <em>Thermus thermophilus </em>(HB-8/ATCC 27634/DSM 579) …
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Protein Engineering via in Vitro Coevolution
… I-SceI with novel DNA cleavage activity. Homing endonucleases have great potential for applications such as targeted gene correction in gene therapy and gene alteration in systems biology and metabolic engineering. However, the limited repertoire of recognition sequences in naturally available …
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A novel CRISPR-Cas9 platform with divergent targeting capabilities
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. The range of sequences that CRISPR endonucleases can recognize, however, is constrained by the need for a specific protospacer adjacent motif …
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Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna
… and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, and double …
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The Association of the Human Cytomegalovirus Genome with the Cellular Nuclear Matrix
… MIE gene was cloned, digested with restriction endonucleases, and the resulting DNA fragments were reacted with nuclear matrices prepared in vitro. Relative to coding region and vector fragments, the regulatory region of the MIE gene bound the in vitro nuclear matrix with the greatest …
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Isolierung und Charakterisierung von Iridoviren
… from digestion of purified viral DNA with the endonucleases EcoRI, BamHI, and HindIII showed marked differences from the profiles of iridoviruses of lower vertebrates (genus Ranavirus), e.g., Rana esculenta Iridovirus and Frog virus 3. Restriction enzyme digests with the endonucleases MspI and …
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Interrogation of CRISPR-Cas targeting specificity for mammalian genome engineering
Class II CRISPR-Cas RNA programmable DNA endonucleases enable high efficiency genome editing across the biological diversity for research, industrial, and biomedical applications. Human genome editing with CRISPR-Cas just recently made its debut in human clinical trials and has immense therapeutic …
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Methods and models of screening genomic variants
… genotype and phenotype. Advances in RNA-guided endonucleases have enabled such pooled screens in human cells. I first consider a model to understand drivers of immune evasion in a pooled knockout screen conducted in an in vitro model of metastatic melanoma. Next, I discuss strategies for scaling …
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Characterization of a 4.0 kilobase plasmid from Pasteurella multocida
… The restriction patterns produced by fourteen endonucleases were used to generate a restriction map. Five restriction enzymes cleaved the plasmid at multiple sites. Two enzymes, Bgl II and Sal I had unique sites on pLAR-1. Twelve of the fifty six strains of P. multocida surveyed contained …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… a particular selected gene using site-specific endonucleases, which include meganucleases, zinc finger nucleases, TALEN (transcription activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are …
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Structural Studies of the Mammalian RNase H2 Heterotrimeric Complex
RNase H enzymes are endonucleases that specifically catalyze the hydrolysis of ribonucleotides in RNA:DNA hybrids. They are classified into type 1 or type 2 based on amino acid sequence similarity, structure, and biochemical properties. Type 1 RNase Hs require a minimum of four ribonucleotides …
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Genetic Study of Lactobacillus Strains Colonizing a Murine Gastric Epithelium
… as probe, and analysis with five restriction endonucleases indicated that these large plasmids belong to at least two groups. DNA sequences homologous to the probing plasmid were detected in six of the strains studied. All of the six contained plasmids of various sizes. Only some of them …
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Intraspecies typing of orthopoxviruses
… recently analysis of genomic DNA by restriction endonucleases has proved a valuable additional way of identifying species and revealing intra-species variations among the orthopox viruses. This thesis reports the results of investigations of poxvirus isolates in which both biological …
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