Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Endocytic trafficking"”.
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Regulation of hepatocyte growth factor receptor endocytic trafficking
… terminating HGFR signaling. Perturbation of HGFR trafficking, either at the level of internalization or during sorting at the early endosome for degradation, leads to oncogenic activation of HGFR. Impaired HGFR trafficking is emerging as a key mechanism for HGFR-induced cancer progression and …
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Role of endocytic trafficking during Dpp gradient formation
… I show that most of GFP-Dpp traffics through endocytic compartments at the receiving epithelial cells, probably recycled through apical recycling endosomes (ARE). Finally, a Dpp recycling assay based on subcellular photouncage of ligand is presented to address specifically the Dpp recycling …
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Characterization of The Ubiquitin Ligase, Ube4B, In Endocytic Trafficking
… growth factor receptor (EGFR) is an RTK whose trafficking through the endocytic pathway through the cell is well-documented. Stimulation of EGFR with its cognate ligand, EGF, prompts EGFR entry into the endocytic pathway and simultaneously activates downstream signal transduction pathways that …
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Endocytic Trafficking of The Amyloid Precursor Protein In Rat Cortical Neurons
… cultured primary neurons, we determined that the trafficking of APP from the endosomal membrane into internal vesicles of late endosome/multivesicular bodies (MVB) is dependent on Endosomal Sorting Complexes Required for Transport (ESCRT) machinery. This implied that APP is ubiquitinated to allow …
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Requirements of Slm Proteins For Proper Organization of Eisosome, Endocytic Trafficking, and Recycling
… inactivated Slm proteins reveals that FM4-64 dye trafficking is inhibited at both permissive and non-permissive temperatures. Due to the fact that the severity in actin disruption is correlated with the severity of endocytic defects, I found that the inactivation of SLM genes led to defects in the …
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Quantitative Analysis of Integrin Trafficking and Focal Adhesion Turnover to Study Integration of Early Endocytic Trafficking, Signaling and Migration
Pages 8-153 are misnumbered as pages 9-154.
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Cytokine engineering through ligand/receptor dynamics : a study on granulocyte colony-simulating factor
… cell-surface receptors, these complexes undergo trafficking processes that often attenuate the intracellular signals through internalization and degradation. Additionally for GCSF, the bloodstream neutrophils represent a further negative feedback mechanism that limits the potency of the drug, as …
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Endosomal Trafficking and Exosomal Secretion of Egfrviii
… <p>These results reveal novel aspects of the endocytic trafficking of EGFRvIII that underlie its reduced degradation and the mechanism by which it is packaged into exosomes for secretion. Moreover, I show that EGFRvIII secretion on exosomes can facilitate changes in the tumor microenvironment …
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Biological studies of organellar (Na⁺,K⁺)/H⁺ exchanger NHE7
… NHE7 binding proteins and that SCAMPs regulate endocytic trafficking of NHE7 from the recycling endosomes to the trans-Golgi network (TGN). In agreement with this finding, I found that NHE7 can also be targeted to the plasma membrane and then internalized. Caveolins, structural proteins for …
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Function and Regulation of the Cell Fate Determinant Numb in Polarized Epithelial Cells
… an adaptor protein that functions in regulating endocytic trafficking events. Numb was originally identified in Drosophila as an asymmetrically localized cell fate determinant, and was subsequently found to be conserved in vertebrates. In mammalian polarized epithelial cells, Numb is distributed …
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The Interplay of Signal Transduction and Membrane Traffic of the Macrophage Colony Stimulating Factor Receptor (MCSFR) Controls Macrophage Growth
… we characterized MCSFR internalization, endocytic trafficking, and signaling regulation by new fluorescent microscopy and conventional biochemistry methods. In this work we discovered a novel cellular mechanism regulating MCSFR signaling. Specifically, we showed that the MCSFR is …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
Endocytic recycling is the process by which cells recycle endocytic materials from the trans-Golgi network or endosome back to the cell membrane, as opposed to being targeted to the vacuole for degradation. Ypt31p and Ypt32p GTPases are known to regulate the function of F-box protein Rcy1p, which …
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Activation of lipid kinase VPS34 by Rab GTPases
… II, which act as key regulators of autophagy and endocytic trafficking, respectively. Complex I consists of VPS34, VPS15, Beclin 1 and ATG14L, whereas complex II contains UVRAG instead of ATG14L. VPS34 complex activity is regulated by membrane properties and by members of the Rab family of small G …
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A Systems Biology Approach to Study Type III and Type IV Bacterial Effector Properties
… molecules in usurping the tightly regulated endocytic trafficking pathway were developed. These tools were then used to identify and characterize the location at which three bacterial effectors EspG, VirA, and IpaJ disrupt the global secretory pathway. Lastly, the effector library was …
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A role for HSC70 in regulating antigen trafficking and presentation during macronutrient deprivation
… activity increased with macronutrient stress, endocytic trafficking and proteolytic turnover of the BCR was impaired. Addition of high molecular mass macronutrients restored endocytosis and antigen presentation, evidence of tightly regulated membrane trafficking dependent on macronutrient …
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Analysis of trafficking dynamics and cellular response in the interleukin-2 ligand/receptor system
… in a T cell proliferation assay. Dedicated trafficking studies show that endocytic trafficking of this 20 I variant might be responsible for this increased potency, as a significantly increased fraction of internalized 201 is sorted to recycling instead of to lysosomal degradation. …
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Characterizing Roles of Host Cell Trafficking Factors in HIV Assembly and Release
… host cell Rab GTPases, to investigate the post-endocytic trafficking of Env in physiologically relevant CD4+ T cell lines. We found that endocytosed Env traffics to Rab14+ late endosomes and lysosomes, where it is retained mostly without being degraded, and we showed the first direct evidence …
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Trafficking motifs in potassium channels
… is of fundamental importance to investigate the trafficking mechanisms that regulate the surface density of these channels. Techniques in cell biology, molecular biology and biochemistry were employed to identify the molecular basis of Sar1-GTPase dependent ER exit of the KATP and hERG channels …
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Identification and Characterization of Effectors/Binding Molecules for the Small GTPase Rab15
Endocytic trafficking is a key mechanism for regulating receptor availability on\r\nthe plasma membrane as well as receptor degradation. Clathrin-dependent endocytosis\r\ninvolves receptor internalization into early endosomes. Here internalized receptors are\r\nsorted for degradation in lysosomes, …
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Regulation of synaptic structure and function at the Drosophila neuromuscular junction
… I also describe my studies on how regulation of endocytic trafficking controls synaptic growth during neuronal development. To identify regulators of synapse assembly, I carried out a large-scale EMS mutagenesis screen of the second chromosome. From this screen I identified a mutation in actin …
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