Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Embryonic Stem Cells (ESCs)"”.
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System to study colony-colony interactions in embryonic stem cells
… regulatory factors involved in self-renewal of embryonic stem cells (ESCs). Recent studies suggested that different autocrine factors exist in murine ESCs (mESCs) and can influence their self-renewal and proliferation, predominantly in deficiency conditions. These observations were investigated …
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The feasibility of interspecific rescue of endothelial/hematopoietic lineage deficiency
… mouse and rat to test the possibility that embryonic stem cells (ESCs) from one species are able to survive and develop in a blastocyst from a difference species. Then we intended to make a rat-mouse chimera with an endothelium/hematopoietic system from a rat genetic background and the other …
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Mitochondrial DNA replication in pre-implantation embryonic development
All eukaryotic cells possess mitochondrial DNA (mtDNA), which is maternally inherited through the oocyte, its replication being regulated by nuclear-encoded replication factors. It was hypothesised that mtDNA replication is highly regulated in oocytes, pre-implantation embryos and embryonic stem …
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Quantitative dynamic modeling of transcriptional networks of embryonic stem cells using integrated framework of Pareto optimality and energy balance
Embryonic Stem Cells (ESCs) are pluripotent and thus are considered the "cell type of choice". ESCs exhibit several phenotypic traits (e.g., proliferation, differentiation, apoptosis, necrosis, etc.) and when differentiated into a particular lineage they can perform an array of functions (e.g., …
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Sox2 co-occupies distal enhancer elements with cell-type-specific POU factors to specify cell identity in embryonic stem cells and neural precursor cells
… distinct cellular states, that of pluripotent embryonic stem cells (ESCs) and multipotent neural progenitor cells (NPCs), but what common or distinct roles Sox2 may play in these cell types not fully understood. Further, the molecular mechanisms by which Sox2 can specify two distinct cell …
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Comparative Analysis of Antiviral Responses in Mouse Embryonic Stem Cells and in Mesenchymal Stem Cells
<p>Embryonic stem cells (ESCs) are cells that have unlimited capacity for selfrenewal and differentiation. These properties make ESCs a great cell source for application in regenerative medicine. When used for cell therapy, ESC-derived cells could be placed in a wounded area that is likely to be …
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Revealing a Non-canonical Role of Anti-apoptotic MCL-1 in Early Embryonic Development
… revealed that Mcl-1 deficiency leads to embryonic lethality around E3.5 during peri-implantation stage. Strikingly, the study did not detect any increase in apoptotic cells of the blastocyst, indicating a function of MCL-1 beyond regulating apoptosis. Our previous studies revealed an …
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Attenuated Responses To Inflammatory Cytokines In Mouse Embryonic Stem Cells: Biological Implications And The Molecular Basis
<p>Embryonic stem cells (ESCs) have attracted intense interest due to their great potential for regenerative medicine. However, their immune property is an overlooked but a significant issue that needs to be thoroughly investigated not only to resolve the concern for therapeutic applications but …
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Microfluidic perfusion culture for controlling the stem cell microenvironment
In multicellular organisms, cells do not exist in isolation but communicate with other cells via extracellular signaling molecules, many of which diffuse into the microenvironment. More than most cell types, embryonic stem cells (ESCs) are critically sensitive to their microenvironment, which plays …
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Gcn5 Impacts Fgf Signaling At Multiple Levels and Activates C-Myc Target Genes During Early Differentiation of Embryoid Bodies
… developmental processes remains largely unclear. Embryonic stem cells (ESCs) are self-renewing, pluripotent, and have the abilities to generate almost all cell types in adult tissues. The dual capacity of ESCs to self-renew and differentiate offers unlimited potential for studying gene regulation …
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The Role of p66Shc in Stem Cells: Signalling, Identity and Apoptosis
… apoptosis, the physiological roles of p66Shc in stem cells remain poorly defined. This thesis examines the role of p66Shc in signalling, apoptosis, and extracellular matrix (ECM)-dependent behaviour in embryonic stem cells (ESCs) and neural stem cells (NSCs). In ESCs, deletion of p66Shc enhanced …
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Lack of Inflammatory Response in Mouse Embryonic Cells to Listeria Infection
… cell types within mouse blastocysts, including embryonic stem cells (ESCs) and trophoblast stem cells (TSCs), exhibit remarkable resilience against the cytotoxic effects associated with viral infections and embryotoxic cytokines, such as TNFα and IFNγ, both of which are known to hinder …
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Identification of the ERK5 Kinase as a Key Regulator of Embryonic Stem Cell Pluripotency
Embryonic Stem Cells (ESCs) can self-renew or differentiate into all specialised cell types in the adult body, a phenomenon known as pluripotency. Distinct pluripotent states have emerged as a key concept in ESC biology; the naïve state safeguards selfrenewal whilst primed pluripotency provides a …
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Downregulation of reproductive homeobox gene 6 (RHOX6) interferes with male germ cell differentiation
… the Rhox family, is expressed in primordial germ cells (PGCs) in the developing gonad and the placenta, although the biological function of Rhox6 in determination of PGCs and placental cells remains unknown. To investigate the Rhox6 function, we generated male chimeras by injection of the …
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The Generation of a Candidate Axial Precursor in Three Dimensional Aggregates of Mouse Embryonic Stem Cells
Textbook accounts of vertebrate embryonic development have been based largely upon experiments on amphibian embryos, which have shown that the tissues of the trunk and tail are organised from distinct precursors that existed during gastrulation. In the mouse and chick, however, retrospective clonal …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… despite being a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells (PGCs), the early embryo and in naïve embryonic stem cells (ESCs). Genome integrity is crucial during early development, as the germline DNA …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… cell must produce all of the different types of cells in the adult organism. What are the regulatory mechanisms required to coordinate the necessary gene expression networks for this process? Polycomb group (PcG) proteins are epigenetic regulators necessary for proper differentiation of cells and …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
… genes, such as germ-line related genes in embryonic stem cells (ESCs) (3). During intestinal homeostasis, chromatin modifiers play important roles in maintaining transcriptional signatures of specific cell types. Our group has already demonstrated that RING1A/B are necessary to maintain …
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New Tools for Real-Time Study of Embryonic Development
<p>Embryonic development represents one of the most complex and dynamic cellular processes in biology, and plays vital roles in understanding of functions of embryonic stem cells (ESCs) and design of ESC-based therapy. Conventional assays and fluorescence-based imaging methods have been widely used …
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The Role of TET Proteins in the Epigenetic Regulation of Neural Gene Expression and Behavior
… (Tet3Δe7-9). As Tet1 is also highly expressed in embryonic stem cells (ESCs), we generated Tet1 homozygous deleted ESCs through sequential targeting to compare the function of Tet1 in the brain to its role in ESCs. To test our hypothesis that TET proteins epigenetically regulate transcription of …
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