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Showing 1 to 20 of 168 for “"Electroporation"”.
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Dynamic Electroporation Modelling
… occupy. Conventional biotechnology processes of electroporation make use of unipolar electric field pulses, which are known to generate undesirable conditions such as asymmetrical electropermeabilization. These electrical protocols also contribute to lower efficiencies in electroporation based …
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Modeling cell and tissue electroporation
… of the basic mechanisms of interaction. Electroporation, in which transient, aqueous pores form in lipid bilayers, is one fundamental mechanism by which large electric fields may alter biological systems. Here cell and tissue electroporation models are presented that are based on the …
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Effects of Irreversible Electroporation and High-Frequency Irreversible Electroporation for the Treatment of Breast Cancer
… methods, minimally invasive, non-thermal, focal electroporation-based ablation therapies are being investigated for the treatment of BC. Irreversible Electroporation (IRE) delivers a series of long, monopolar electrical pulses via electrodes inserted directly into the targeted tissue which …
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PEDOT:PSS Enhanced Electroporation for Cell Therapy Manufacturing
… such as viral vectors, lipid vectors, electroporation, microinjection, and chemical transfection, among others. Miniaturization and automation of these techniques are essential for scaling the complexity of cellular therapies without concurrently scaling the costs of research and …
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Surface tension and electroporation of lipid bilayers
Electroporation of lipid bilayers is widely used in DNA transfection, gene therapy, and targeted drug delivery and has potential applications in water desalination and filtration. A better, more thorough molecular understanding is needed, however, before such devices can be effectively used and …
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The pathways and mechanisms of skin electroporation
Thesis (Sc.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1999.
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Finite element modeling of high frequency irreversible electroporation
High frequency irreversible electroporation (HFIRE) is a minimally invasive, non-thermal method of soft tissue ablation that has potential to effectively treat tumors and atrial fibrillation without the severe muscular contractions that occur with monophasic pulses. The technology is relatively …
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Electrical characterization of electroporation of human stratum corneum
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1994.
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Rapid single-cell electroporation for labeling organotypic cultures
Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in …
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Development of microfluidic pipette tips for automated electroporation
… maximum output performance of the cuvette electroporation process was 2.5x10⁹ CFU/([mu]gDNA). The performance of the microfluidic tips is 2.5X the output efficiency of the cuvettes at the same applied potential difference and DNA concentration. Further experiments studied the effects of …
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A unified model of electroporation and molecular transport
… transmembrane voltage, a phenomenon termed electroporation. These pores facilitate electrical and molecular transport across cell membranes that are normally impermeable. By applying pulsed electric fields to cells, electroporation can be used to deliver nucleic acids, drugs, and other …
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Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels
Electroporation is an efficient, low-toxic physical method which is used to deliver impermeant macromolecules such as genes and drugs into cells. Genetic modification of the cell is critical for many cell and gene therapy techniques. Common electroporation protocols can only handle small volumes of …
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Microfluidic systems for cell transfection using sonoporation and electroporation
Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation …
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DEVELOPMENT OF A GENERAL LETHAL CRITERION FOR IRREVERSIBLE ELECTROPORATION
Irreversible electroporation (IRE) is a non-thermal and promising technique for tumor ablation that generates an ablation zone producing permanent nanopores in the membrane of cells, thus leading to cell death. The ablation zone is determined with a designed treatment plan, including a selection of …
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Electroporation of tissue and cells for drug delivery applications
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1994.
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Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation
… manage and prevent these cancers. Irreversible electroporation is a new non-thermal focal ablation therapy utilizing short, pulsed electric fields to damage cell membranes leading to cell death. The therapy is minimally invasive, involving the insertion of needle electrodes into the region of …
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Improvements in Pulse Parameter Selection for Electroporation-Based Therapies
Irreversible electroporation (IRE) is a non-thermal tissue ablation modality in which electrical pulses are used to generate targeted disruption of cellular membranes. Clinically, IRE is administered by inserting one or more needles within or around a region of interest, then applying a series of …
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Advancements in Irreversible Electroporation for the Treatment of Cancer
Irreversible electroporation has recently emerged as an effective focal ablation technique. When performed clinically, the procedure involves placing electrodes into, or around, a target tissue and applying a series of short, but intense, pulsed electric fields. Oftentimes, patient specific …
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High aspect ratio electrodes for high yield electroporation of cells
Electroporation is a widely used process in cell biology studies. It uses an electric field to create pores on the cell membrane in order to either insert exogenous molecules inside the cells or disrupt the cell membrane to kill the cells. Current micro-fluidic electroporation devices use the …
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