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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 41 for “"Electrophoretic Mobility Shift Assay"”.

  1. Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin

    Milligan, Sarah LThe University of Texas Medical Branch at Galveston2019

    … further confirmed using an in vitro SUMOylation assay with recombinant TRP47, and native polySUMOylated TRP47 was immunoprecipitated from E. chaffeensis-infected cell lysates. TRP47 was detected in host cell nuclei primarily at 24 and 48 hours post-infection by immunofluorescence microscopy. A …

    utmb Repository record

  2. Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

    Kibler, Clayton EugeneThe University of Texas Medical Branch at Galveston2021

    … dependent on a variant MYND-binding domain. An electrophoretic mobility shift assay (EMSA) demonstrated that TRP47 directly binds host DNA via its tandem repeat domain. Utilizing chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) with E. chaffeensis-infected …

    utmb Repository record

  3. Strategies of overexpressing retinoid X receptor and pregnane x receptor for functional studies

    Bunton, Chandra Zaneta2018

    … analysis. RXR function was assessed by electrophoretic mobility shift assay (EMSA) and proved to effectively form a DNA binding heterodimer with PXR. These studies involving RXR and PXR demonstrate that these proteins can be efficiently produced in a functional manner utilizing an …

    u-pacific Repository record

  4. Signaling Pathway Inhibitor Library Screening Reveals B-Catenin/TCF4 as a Novel Telomerase Regulator in Cancer cell lines

    TOH LING LING, JOELLE2013

    … also found and evidences from promoter studies, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay, showed that ?-catenin/TCF4 bind to hTERT promoter in vivo and in vitro. Taken together, this is the first study has shown that Wnt signaling regulates telomerase via the …

    nus Repository record

  5. Significant Association of ADORA3 Genetic Polymorphisms with Aspirin-Intolerant Urticaria in a Korean Population

    남, 은주2011

    … in ADORA3 were analyzed by luciferase reporter assay, electrophoretic mobility shift assay (EMSA), and real-time PCR for the mRNA expression in PBMC of AIU patients. RESULTS: The haplotype [T-1050C-564] frequency of ADORA3 gene showed significantly higher in AIAU group than in AICU and NC groups …

    ajou Repository record

  6. Genetic Effect of ALOX15 Polymorphisms on Aspirin-Exacerbated Respiratory Disease

    송, 영신2011

    … were analyzed by luciferase reporter activity assay, electrophoretic mobility shift assay (EMSA), and enzyme activity assay in red blood cell (RBC) lysates. RESULTS: No significant difference of the genotype frequency in ALOX15 genetic polymorphism was addressed. Peripheral blood total …

    ajou Repository record

  7. TDI 천식 기도염증반응과 관련된 활성산소의 역할

    김, 성호2010

    … 7 1. Epithelial cell culture and tTG activity assay 7 2. Intracellular reactive oxygen species (ROS) measurement 8 3. Immunoblot analysis 8 4. In vitro cross-linking reaction assay 9 5. Statistical analysis 9 B. Method 10 1. Materials 10 2. Cell culture 10 3. Real-Time polymerase chain reaction …

    ajou Repository record

  8. Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling

    Delucchi, Anthony Benjamin2001

    … analysis. C∆418 function was assessed by electrophoretic mobility shift assay (EMSA) and proved to effectively form a DNA binding heterodimer with ARNT. In addition the functionality of C∆418 was assessed by co-precipitation showing that the ThioHis-produced C∆418 was indeed able to …

    u-pacific Repository record

  9. Identification and Characterization of the Cis-acting Elements Around the Murine cd4 Cnhancer

    Dong, Xin

    … chloramphenicol acetyltransferase (CAT) assay. Two novel <em>cis</em>-acting elements with silencer function were discovered by Bal 31 deletions. Their silencer functions were further confirmed by subcloning them at different positions and different orientations relative to CD4 promoter. …

    loma-linda Repository record

  10. Significant Association of IL-18 Genetic Polymorphisms with Aspirin-Intolerant Urticaria in a Korean Population

    손, 진경2011

    … of IL-18 were examined by luciferase reporter assay, electrophoretic mobility shift assay (EMSA) and neutrophil chemotactic assay. RESULTS: The minor allele frequency of IL-18 at -607 A>C and haplotype 1 [C-607G-137] showed significantly higher in AIAU than in NC groups(p=0.019, for AIAU vs NC; …

    ajou Repository record

  11. Genetic and biochemical interactions between the DNA replication initiator and a chromosome architecture protein in Bacillus subtilis

    Seid, Charlotte AMassachusetts Institute of Technology2014

    … DnaA and Rok using purified proteins in an electrophoretic mobility shift assay. DnaA depended on Rok for association with a DNA probe, recapitulating the dependence observed at this chromosomal region in vivo. DnaA and Rok are both transcription factors and regulate some of the same genes. …

    mit Repository record

  12. Characterization of Gene Expression During Adenosine 3':5'-Cyclic Monophosphate Induced Neuroendocrine Differentiation in Human Prostatic Adenocarcinoma

    Goodin, Jeremy LeeVirginia Tech2002

    … the promoter region for this gene was analyzed. Electrophoretic mobility shift assay, antibody supershift assays, site-directed mutagenesis, and luciferase reporter gene analysis were employed to authenticate the roles of several transcription factors in the regulation of the HRS3a gene. Two …

    vt Repository record

  13. Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis

    Ho, Ngoc Anh ThuResearchSpace@Auckland2017

    … by multiple biochemical techniques (i.e. electrophoretic mobility shift assay, surface plasmon resonance and intrinsic fluorescence quenching assay). Protein-DNA binding assays have demonstrated that the 21-amino-acid extension at the N-terminus of KstR imposes a positive but non-essential …

    auckland-ms Repository record

  14. GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae

    Sheen, Hyuk Ho2018

    … as bait RNA and a streptotag-based RNA pull-down assay, GCD10 and GCD14 were identified as specific 5UTR-binding proteins. Subsequent electrophoretic mobility shift assay (EMSA) shows that GCD10 and GCD14 proteins bind directly to the 5 UTR and both subunits are required for the binding. To …

    york Repository record

  15. Functional analysis of MYB112 transcription factor in the model plant Arabidopsis thaliana

    Lotkowska, Magda EwaUniversität Potsdam

    … micro-arrays and qRT-PCR) and transactivation assays in leaf mesophyll cell protoplasts. We show that MYB112 binds to an 8-bp DNA fragment containing the core sequence (A/T/G)(A/C)CC(A/T)(A/G/T)(A/C)(T/C). By electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation coupled …

    potsdam-diss Repository record

  16. Genetic Effect of MBL2 Polymorphisms in Patients with Diisocyanate-induced Occupational Asthma

    배, 수진2013

    … were genotyped by TaqMan allelic discrimination assays using TaqMan probes. The serum MBL levels were detected using enzyme-linked immunosorbent assay (ELISA). The functional variabilities of the promoter polymorphisms were analyzed by luciferase reporter assay and electrophoretic mobility shift …

    ajou Repository record

  17. DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus

    Wowor, Andy James BudimanBiological Sciences2022

    … For both proteins, binding of ss-DNA shifts from weakest to tightest binding of the three structures as the salt concentration increases. Both Klenow and Klentaq release 2-3 more ions when binding to pt-DNA and ds-DNA than when binding to ss-DNA. Both of these non-sequence specific …

    lsu-thes Repository record

  18. Genetic effect of histamine N-methyltransferase (HNMT) polymorphism on chronic urticaria with aspirin hypersensitivity

    강, 영미2008

    … the SNP-IT and TaqMan fluorogenic 5’nuclease assay, respectively. The functional effect of polymorphisms of -465T>C, -413C>T and 939 A>G was analyzed by luciferase reporter assay, electrophoretic mobility shift assay (EMSA), 3’-UTR stability, enzyme activity assay and histamine release from …

    ajou Repository record

  19. Thymoquinone is a Novel Ligand which Activates Neu4 Sialidase to Promote a Pro-Inflammatory Response

    Finlay, Trisha2009

    … cells and human fibroblast cells using an assay that measures the cleavage of the sialidase specific fluorescent substrate 2’-(4-methylumbelliferyl)-α-DN-acetylneuraminic acid (4-MUNANA). The cleavage of 4-MUNANA causes the release of free 4-methylumbelliferone, which fluoresces at 450nm …

    queens Repository record

  20. HIF, HAPE, and Hilltop rats: a paradox unfolding

    Engebretsen, Barbara J., author, Tucker, Alan, advisor, Miller, C. W., committee member, Gotshall, Robert, committee member, Cordain, Loren, committee memberColorado State University. Libraries2003

    … protein extracts were assessed by immunoblot assay for HIF-1α, HIF-1β, NOS II, endothelial NOS (NOS III), and VEGF expression. Electrophoretic mobility shift assay and enzyme linked immunosorbant assay determined HIF-1 DNA-binding activity. Results: Hilltop rats had lower BFww/dw after 18 …

    colostate Repository record

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