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Showing 1 to 20 of 1056 for “"Electrophoresis"”.
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Velocity-difference induced focusing in capillary electrophoresis and preparative capillary electrophoresis
… (V-DIF) with a dynamic pH junction in capillary electrophoresis (CE) using a sample with a pH different from that of the background electrolyte (BGE) was developed in our group, but the mechanism was not well understood. In this work, the mechanism of this focusing technique was first studied …
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Gel electrophoresis for elemental speciation purposes
This work has shown that gel electrophoresis is a relevant fractionation method for trace elements bound to or complexed by macromolecules. Furthermore, detection methods of the trace elements that do not rely on the use of radiotracers were applied, making this combination relevant for materials …
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Selectivity and detection in capillary electrophoresis
… and detection limitations in capillary electrophoresis. A more practical design of an electrochemical detector is introduced with simultaneous on-line UV detection¹, for the selective detection of a number of pungent and neurological compounds, the piperines and the capsacinoids. …
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Square-wave voltammetry detection for capillary electrophoresis
… the development and application of capillary electrophoresis (CE) has matured significantly in the last two decades, its application to trace analysis has been limited by The lack of a generally applicable, Sensitive detection method. This Ph.D. project's aim was to develop such a method using …
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Novel absorption detection techniques for capillary electrophoresis
<p>Capillary electrophoresis (CE) has emerged as one of the most versatile separation methods. However, efficient separation is not sufficient unless coupled to adequate detection. The narrow inner diameter (I. D.) of the capillary column raises a big challenge to detection methods. For UV-vis …
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The application of electrophoresis to separation processes
Thesis: B.S., Massachusetts Institute of Technology, Department of Chemical Engineering, 1958
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A stain-free detection system for electrophoresis
… a novel stain free detection system for slab gel electrophoresis is examined. Currently, stained techniques are used to identify electrophoretic bands in gels. The stains utilized in these methods involve health risks since they are mutagenic. Also stains like EtBr are intercalating agents meaning …
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Non-linear electrophoresis of ideally polarizable particles
This thesis investigates the non-linear regime of electrophoresis, in particular the variation of electrophoretic velocity with electric field at high field strengths. Known theoretical approaches to the problem accounting for ion steric effects, dielectric decrement effects and charge-induced …
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Study of disposable microdevices for DNA electrophoresis
… feasible. The chip was designed to perform gel electrophoresis, specifically of DNA fragments for either sequencing or identification purposes. With a disposable version of such a chip, constraints on the gel type are relaxed and lifetime issues become nonexistent. Such a chip was created using …
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A doppler electrophoresis instrument for macromolecular characterizations
Electrophoresis is the technique used to characterize proteins, oligonucleotides, and DNA. Methods employed to date include gel and capillary electrophoresis. Most samples can be characterized by these methods. However, large DNA molecules do not separate well with either method. A newer …
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Using Capillary Electrophoresis for High-Throughput Molecular Screening
… enrichment (SELEX) and nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). However, these stand-alone techniques require multiple rounds of selection and produce a limited number of each unique aptamer. SELEX has the added disadvantage of heavy resource consumption. Thus, …
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Capillary Zone Electrophoresis Studies of Sulfur Containing Compounds
… a new approach, the use of Capillary Zone Electrophoresis (CZE) for the analysis of these types of ions as well as the closely related thiophenium ions. CZE offers an unprecedented advantage in that separations can be employed for speciation or quantitation of complex mixtures by using the …
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Fundamental Capillary Electrophoresis: An Evaluation of Electrokinetic Sampling
<p>Capillary electrophoresis has become a powerful separation technique in fields of biochemical separations, inorganic ions, and chiral separations. The technique has experienced exponential growth since the historic publication of Zone Electrophoresis in Open-Tubular Glass Capillaries by Lukacs …
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THE DETERMINATION OF TRACE METALS BY CAPILLARY ELECTROPHORESIS
The development of a capillary electrophoresis (CE) method for the determination of inorganic and organo mercury species as their dithizone sulphonate (DzS) complexes using coated capillary columns is described. The complexes were pre-formed before injection and detection was by direct measurement …
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Core-based hyperbranched polyethyleneimine coating in capillary electrophoresis
In capillary electrophoresis (CE), chemical and dynamic modification of a fused silica surface can be used to control the electroosmotic flow (EOF), to minimize the interaction of analytes with the capillary surface and to modify the selectivity. , core-based hyperbranched polyethyleneimine (CHPEI) …
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Further investigation of capillary electrophoresis for pharmaceutical analysis
… In this context the potential of capillary electrophoresis (CE) is established through a study of the current literature. Capillary electrophoresis method is developed and optimised for alkylxanthines. The achieved separation for these analytes stands out for its high efficiency and …
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Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection
Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino …
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