Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Electropherogram"”.
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The study of capillary electrophoresis: polyimides, nylon and non-aqueous analysis
… compositional analysis is described. The electropherograms of decomposed products from polyimide are determined by the structure of monomers from which a polyimide is made. In most cases, it is difficult to know structures for all peaks that appear in the electropherogram. But different …
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Investigations of Chemiluminescence Detection Parameters for Kinetic Analysis and Capillary Separations
… camera imaged the resultant CL emission, and an electropherogram was reconstructed. This post-column detection scheme decouples the separation parameters from detection and allows longer lived CL reagents to be used with fast separations.
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Signal processing for DNA sequencing
… is the conversion of the electrical signal-the electropherogram--collected by the sequencing equipment to a sequence of letters drawn from ( A,TC,G ) that corresponds to the sequence in the molecule sequenced. This work formulates the problem as a pattern recognition problem, and observes its …
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic Assignment Tool (PAT). Species belonging to the Class Bacilli were the most abundant followed by unclassified species.</p> <p>Two road killed river otters were necropsied to recover brain and blood …
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A mathematical model of polymerase chain reaction induced stutter
… on understanding stutter present in capillary electropherogram readouts as this methodology forms the basis of current DNA fingerprinting. The readouts come from taking samples of various initial template masses of DNA from different individuals, applying polymerase chain reaction (PCR) to the …
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Identifying, Monitoring, Quantifying and Converting Algae to Bio-Fuels in Bio-Reactors
… DNA amounts with the area under the curve in an electropherogram. This primer also distinguished between and quantified each species. CE-SBE demonstrated great potential for quantification of algae with difficult morphologies, and algae grown in a co-culture photo-bioreactor.</p>
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Interaction of phthalazines with molybdenum hydroxylases. Phthalazine and its 1-substituted derivatives as substrates, inhibitors and inducers of aldehyde oxidase and xanthine oxidase, both in vitro and in vivo.
… band of enzyme activity is apparent on the electropherogram. The Km value for phthalazine is significantly reduced with enzyme prepared from phthalazine treated rabbits, indicating that a form of the enzyme with a high affinity for phthalazine may have been induced. 1-Hydrazinophthalazine …
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Studies of Enzyme Kinetics and Inhibition Through Capillary Electrophoretic Enzyme Assays
… depletion of NADH is monitored, resulting in an electropherogram that is inverse relative to previous EMMA methods. The remainder of the dissertation focuses on the use of magnetic beads with immobilized enzymes for heterogeneous on-line enzyme assays. A study of bead surface chemistry effects on …
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Short tandem repeat (STR) profile authentication via machine learning techniques
… at both the channel and profile levels of STR electropherograms. A number of supervised and unsupervised machine learning algorithms were then used to predict whether a given STR electropherogram was authentic or synthesized by laboratory techniques. With the aid of the LNKnet machine learning …
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Square-wave voltammetry detection for capillary electrophoresis
… normally observed and produced negative electropherogram peaks. Using this as a detection method, reasonable detection limits (~1[mu]M, SN = 3) were obtained for analytes that did not have UV-chromophores. When this detection method was applied to analytes with [pi]-electron density (i.e. …
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Capillary electrophoresis instrumentation and applications for the analysis of neural systems
… systems by substantially decreasing the electropherogram complexity and reducing background interference. Many tryptophan and tyrosine metabolites present in biological systems are amenable to LINF detection. An enhanced wavelength-resolved CE-LINF instrument was developed in an effort to …
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Blood on FTA™ Paper: Does Punch Location Affect the Quality of a Forensic DNA Profile?
… involves observation of data in the form of an electropherogram, and subsequently annotating a DNA “profile” from an individual or from the evidence. The profile obtained from the evidence can be compared to reference profiles deposited in a national DNA database, which may include the potential …
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The development of PCR internal controls (PICs) for forensic DNA analysis
… of fragments were detected as red peaks on the electropherogram flanking the human DNA profile. To study the behaviour of the markers within the system and their effect on the performance and sensitivity of the assay, the PICs were used during the amplification of human DNA of different quantity …
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Reconstruction of major male and female lineages of the Strand Muslim community
Initially, a pilot study was carried out in order to reconstruct the major paternal and maternal lineages of the Muslim population living in the Cape metropolitan area. The Study has shown the ability of molecular genetic tools to give insight into the origins and history of local communities. The …