Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Electron tomography"”.
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Electron tomography of defects
Tomography of crystal defects in the electron microscope was rst attempted in 2005 by the author and colleagues. This thesis further develops the technique, using a variety of samples and methods. Use of a more optimised, commercial tomographic reconstruction program on the original GaN weakbeam …
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Investigating Microtubules Inside Cells with Cryo-Electron Tomography
… neuronal microtubules remain unclear. Cryogenic electron tomography allows for visualization and structure determination of purified or cellular specimen in their native-like, vitrified state. I used this method in combination with subtomogram averaging to study intracellular microtubules and …
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Understanding nanomagnetism from all angles: Developments in magnetic electron tomography
This thesis aims to develop transmission electron microscopy (TEM) techniques to study magnetic nanomaterials in three dimensions (3D). At the nanoscale, magnetic materials exhibit various intriguing behaviours such as topological protection in skyrmions (which may be used in next-generation …
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Three-Dimensional Morphology of Polymer Nanocomposites Characterized by Transmission Electron Tomography
Electron tomography is an invaluable technique with the capability of carrying out thorough 3D structural, chemical and morphological characterization of materials at nanometer scale. Tilting range, increment and reconstruction algorithms are three of the main factors affecting the quality of …
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Visualisation of innate immune signalling complexes in cells by cryo-electron tomography
… of MAVS. I optimised a cryo-correlative light electron microscopy (cryo-CLEM) workflow to attain high-resolution structural insights into the ASC signalling complexes in cells. My cryo-ET reconstructions, obtained in unstained and fully hydrated conditions, show that the speck is formed of a …
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Investigating the cytoskeleton and microtubule-based transport with in situ cryo-electron tomography
… cargo interaction are not well described. Cryo-electron tomography (cryo-ET) can be applied to cellular samples to resolve macromolecules spatially at nanometer resolution. When many copies of a target are present, sub-tomogram averaging (STA) can be applied to improve resolution. I aimed to use …
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A CORRELATIVE CRYO-ELECTRON TOMOGRAPHY APPROACH TO STUDY BRD4-NUT CHROMATIN CONDENSATES ACROSS SCALES
… compatibility with active transcription. Cryo-electron tomography (cryo-ET) is suitable for conducting such analyses, as it allows molecular-resolution imaging of cellular structures within their native context. However, methodological challenges limit the straightforward application of cryo-ET …
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Optimización en problemas de tomografía electrónica y localización de concentradores con asignación única optimization in electron tomography and single-allocation hub location problems
En esta tesis doctoral se abordan problemas de optimización matemática procedentes de dos áreas principales: Tomografía Electrónica y Teoría de Localización. Por este motivo, este manuscrito se puede dividir en dos grandes bloques. En el primero, se han desarrollado una amplia gama de herramientas …
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Spindle organization in three dimensions
… mitotic spindle components was analyzed by electron tomography. This 3-D analysis reveals morphologically distinct microtubule end morphologies in the mitotic spindle pole. These results have structural implications for models of microtubule interactions with centrosomes Third, spindle …
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INVESTIGATION OF ADVANCED IMAGE RECONSTRUCTION ALGORITHMS FOR ELECTRON MICROSCOPY
… optics have made transmission electron microscopy a widespread and essential tool for 2D/3D material characterization at the atomic scale. With the rapid development of hardware and novel experimental techniques, there is an increasing demand for advanced algorithms to work with …
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
… of the overall architecture of the NPC by cryo-electron tomography(cryo-ET) to ~23Å resolution has revealed its size, shape, and course arrangement, but lacks distinguishable protein-protein boundaries and secondary structural details.
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Developmental Changes In The Structure and Composition of The Postsynaptic Density
… are identical. Here, immuno-gold labeling and electron tomography of PSDs isolated throughout development was used to track changes in both the structure and molecular composition of the PSD. State-of-the-art cryo-electron tomography was used to study the fine structure of the PSD during …
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Visualisation of dynein complexes in vitro and inside cells
… also not clear. To understand this, I used cryo-electron microscopy (EM) combined with biochemical analysis and fluorescence microscopy to study dynein in vitro and in cells. In Chapter 2, I present the cryo-EM structure of purified full-length human cytoplasmic-1 in an inhibited state. By making …
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Topological analysis of microtubules during cell division
… topology of microtubules obtained through large electron tomography data. Our results show that the geometry/topology and entanglement of microtubules changes throughout cell division and it depends on the length of the microtubules and their locations. We also detect change in twisting of the …
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Quasi-four-dimensional characterization of dislocation interactions in FCC and HCP systems
… using in situ deformation in a transmission electron microscope, diffraction contrast electron tomography for three-dimensional analysis, and electron backscatter diffraction for orientation determination. In situ deformation experiments were conducted at room temperature and elevated …
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Assessing Blood-Based Laboratory Diagnostics for Alzheimers’s Disease: A Systems Approach
… cerebrospinal fluid (CSF) testing and positron electron tomography (PET) brain imaging, are invasive, costly, and inaccessible to many. Blood-based tests offer a less invasive and more cost-effective alternative, yet they remain underutilized in clinical practice. By conducting a literature …
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Time-resolved and three-dimensional study of dislocation-particle interactions in aluminum and copper alloys
… straining experiments inside the transmission electron microscope, post mortem analysis, and electron tomographic reconstructions. Specifically, diffraction-contrast electron tomography was developed during the course of this work as a 3D imaging technique for crystalline systems such that the …
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Atomic-scale and three-dimensional transmission electron microscopy of nanoparticle morphology
… materials. This thesis addresses transmission electron microscope characterisation of nanoparticle morphology, concerning specifically the crystal- lographic status of novel intermetallic GaPd2 nanocatalysts and advancement of electron tomographic methods for high-fidelity three-dimensional …
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Tomographic reconstruction of the morphology of silicon nanoparticleclusters
… TEM, high resolution TEM and transmission electron tomography techniques. TEM and TEM based tomography provide the means to determine the size and morphology of primary particles and clusters, while the structure of printed macroscopic layers has previously been characterised via small …
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Computational Modeling of Claudin-15 Paracellular Channels
… reduced computational modeling, and cryo-electron tomography (cryo-ET) to address these gaps. Atomistic MD simulations of Cldn-15 strands in opposing plasma membranes demonstrated that lateral flexibility emerges from multiple modular cis interfaces, permitting curvature and branching …
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