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Showing 1 to 5 of 5 for “"Electric field gradient focusing"”.
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Electric Field Gradient Focusing-UV Detection for Protein Analysis
<p>Electric field gradient focusing (EFGF) utilizes a hydrodynamic flow and an electric field gradient to focus and concentrate charged analytes and order them in a separation channel according to electrophoretic mobility. Elution can be achieved by decreasing the applied voltage or increasing the …
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Polymer Microchips for Capillary Electrophoresis and Electric Field Gradient Focusing of Biomolecules
… peak efficiencies of 43,000 theoretical plates. Electric field gradient focusing (EFGF), which uses a combination of pressure-driven flow and an electric field gradient to separate charged species according to their electrophoretic mobilities, was explored for protein analysis. Capillary-based …
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Investigation of Novel Microseparation Techniques
… a concentrated sample plug due to on-column focusing. Factors influencing peak symmetry, resolution and analysis cycle length were investigated. Quantitative analysis of a continuous sample stream is possible under certain operating conditions. Electric field gradient focusing (EFGF) devices …
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Fabrication of Polymeric Microfluidic Devices for Protein Analysis
… buffer ion permeable membrane in a PGMAMMA micro electric field gradient focusing (µEFGF) device. Using the µEFGF device, green fluorescent protein (GFP) was concentrated 4000-fold. Separation of GFP and R-phycoerythrin (R-PE), and selective elution of GFP from a protein mixture containing GFP, …
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Detection of Proteins by Two-Photon Excitation of Native Fluorescence
… µTAS separation methods, the relatively new electric field gradient focusing (EFGF) technique has shown potential. It focuses and separates analytes based on their electrophoretic migration in an opposing hydrodynamic flow. The detection principles that are compatible with µTAS separation may …