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Showing 1 to 12 of 12 for “"Elastin-like polypeptides"”.
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Bioorthogonal Functionalization of Elastin-like Polypeptides
… platforms comprised of a unique biopolymer, elastin-like polypeptide (ELP). This thermally responsive biopolymer is easily recombinantly synthesized, though more biochemically complex ELPs require successful bioorthogonal modification. We designed the unnatural amino acid-containing ELPs …
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Programmable Assembly of Elastin-like Polypeptides Within Droplet Microenvironments
… and consists of programming the assembly of elastin-like polypeptides, an archetypal class of disordered proteins, into model organelles within droplet microenvironments. We synthesized a library of recombinant elastin-like polypeptides, and studied their phase separation and assembly into …
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Expression, Purification, And Characterization Of Elastin-Like Polypeptides Containing Chondroitin Sulphate Binding Domains
… a small-diameter blood vessel. Using recombinant elastin-like polypeptides (ELPs) developed by the Woodhouse laboratory, as well as chondroitin sulphate-specific binding sequences (CSBD1 and CSBD2) determined by the Panitch laboratory, a new elastin-like polypeptide-chondroitin sulphate binding …
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Glycoprotein mimetic materials - synthetic methods and study of viral inhibition properties
… The tyrosine enrichment required to prepare elastin-like-polypeptides (ELP) proteins for diazo coupling reactions prevented protein expression at 96 pentapeptide repeats and above, but maintained yields comparable to other work at 48 repeats and below.
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Directing the Mineralization of Inorganic Nanomaterials using Programmable Macromolecular Building Blocks
… engineered functions. With thermoresponsive elastin-like polypeptides (ELPs) fused to solid-binding peptides, we demonstrate the biomimetic mineralization of highly uniform and positively charged silica nanoparticles that support the one-step assembly of mono- and bi-material superstructures …
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Expanding the genome editing toolbox for biomedical applications
… delivery methods, including DNA origami (DO), elastin-like polypeptides (ELPs), and sperm-mediated delivery. We showed that DO nanostructures were efficiently taken up by zygotes and crossed both the ZP and plasma membrane. In contrast, ELPs and mouse sperm failed to deliver the gene editing …
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Leukocyte Response to Elastin-Like Polypeptide Coatings
… thrombosis. The extracellular matrix protein elastin has been found to be one of the least thrombogenic components of blood vessels, and its purified and recombinant forms have shown reduced thrombogenicity in both in vitro and in vivo models. Biomaterial coatings of elastin-like polypeptides …
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Synthesis and Grafting To of Biomimetic Bottlebrush Polymers
… coatings are found in nature as brush-like glycoproteins that decorate biointerfaces and provide anti-fouling, lubrication, or wear-protection. These molecules contain a surface-adhesive headgroup to anchor macromolecules to a target surface and a bottlebrush polymer that endows the …
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Sortase as a Tool in Biotechnology and Medicine
… this reaction with the unique phase behavior of elastin-like polypeptides, we developed a protocol that produces homogenously-labeled protein-small molecule conjugates using only centrifugation. The same reaction can be used to produce unmodified therapeutic proteins simply by substituting a …
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Inducing Chirality in CdS Nanocrystals with Biomolecules
… the use of intrinsically chiral biomolecules, like peptides and proteins, to generate chiroptical responses in these materials. Chapter 1 reviews the synthetic tunability of CdE NCs and current approaches of synthesizing chiral nanostructures. It then introduces the structural complexity of …
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Understanding Elastin-Like Polypeptide Block Copolymer Self-assembly Behavior
<p>Elastin-like polypeptides (ELPs) are thermally responsive polymers composed of the pentapeptide repeat Valine-Proline-Glycine-X-Glycine where X is any amino acid except proline. ELP diblocks have been engineered by creating two ELP blocks with hydrophilic and hydrophobic guest residues. The …
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Synthesis, nanostructure, and mechanics of thermoresponsively tough biomaterials from artificial polypeptides
… of poly(N-isopropylacrylamide) (PNIPAM) and elastin-like polypeptides (ELPs) was leveraged to assemble nanostructured hydrogels from dual-associative block copolymers. Hybrid protein-polymers or protein fusions were formed by fusing PNIPAM or ELPs to the termini of a soluble artificial …