Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 127 for “"Egress"”.
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Alphavirus Entry, Assembly, and Egress.
North Carolina State University Theses Biochemistry.
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Defining Molecular Mechanism of Egress in Cryptosporidium
… of gene function in the parasite. Merozoite egress is a crucial event in the life cycle of Cryptosporidium that contributes to parasite propagation. However, the molecular mechanisms behind egress remain unknown. We propose to study the genes upregulated in the intracellular stages of …
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A role for myotubularins in vaccinia virus egress
… regions. To study factors affecting IEV egress, a proteomic study was performed with A36, E2 and F12 as bait, and many potential interaction partners were identified. Amongst them, two cellular phosphoinositide (PI) phosphatases, myotubularin related protein (MTMR) 1 and su(var), enhancer …
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Viral and cellular proteins involved in vaccinia virus egress
… motor protein are thought to be involved in IEV egress from the site of wrapping to the cell periphery, although the exact mechanism of movement is unclear. Until recently, A36 was the only known protein to interact with the kinesin-1 motor through kinesin light chain (KLC), but F12 has also been …
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CELLULAR AND MOLECULAR PATHWAYS CONTROLLING SARS-COV2 ASSEMBLY AND EGRESS
… from many other RNA viruses that assemble and egress directly at the plasma membrane. This feature adds an additional layer of complexity to the study of the coronavirus life cycle as, once assembled, virions have to traffic through host intracellular membranes to reach the plasma membrane to …
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Investigating the mechanisms of BK Polyomavirus egress and virus-host interactions.
… this thesis further investigated the non-lytic egress of BKPyV by examining the effects of knocking out cellular genes thought to be involved in bypass of the Golgi apparatus on viral release, known as GORASP1 and GORASP2. It was observed that single CRISPR-mediated knockouts of each gene both …
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The role of heterochromatin markers in lytic human herpesvirus egress and maturation
… in the host nucleus, assemble capsids then egress out of the nucleus, mature through acquisition of tegument proteins and secondary envelopment, and finally bud out of the host cell. The virus must contend with components of the host nucleus to replicate, including the host chromatin. …
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The introduction of adaptive social decision-making in the mathematical modelling of egress behaviour
This dissertation represents an attempt at increasing the behavioural sophistication of evacuation simulations, through the study of evacuation modelling, the development of new behavioural algorithms, their implementation within an existing evacuation model and the testing of the resulting model. …
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Studies of viral and cellular proteins involved in herpes simplex virus type-1 egress
The egress pathway of herpes simplex virus-1 (HSV-1) is a complicated process mediated by co-ordinated activity of several virus glycoproteins. The virions are first assembled and enveloped at trans-Golgi-network (TGN) or endosome membranes and then travel through a guided pathway that is directed …
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High-throughput functionalization of the Toxoplasma kinome uncovers a novel regulator of invasion and egress
… was shown to be a regulator of invasion and egress. I call the kinase Store Potentiating/Activating Regulatory Kinase (SPARK), based on its impact on intracellular Ca2+ stores. Despite homology to mammalian PDK1, SPARK lacks a lipid-binding domain, suggesting a rewiring of the pathway in …
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Characterizing the Role of the Herpes Simplex Virus Type 2 UL21 Protein in Viral Nuclear Egress
… primary envelopment of capsids during nuclear egress, however the precise mechanism by which it does this is unclear. To investigate the role of UL21 in HSV-2 nuclear egress, we focused on events upstream of primary envelopment. First, we examined the role of UL21 in disrupting the localization …
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Computational modelling of agent based path planning and the representation of human wayfinding behaviour within egress models
The focus of this thesis is on wayfinding within buildings from an evacuation/circulation modelling perspective. Majority of the existing evacuation models simplify the process of wayfinding by assigning the shortest path to all agents. This is not a realistic representation of the actual route …
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Experimental and Numerical Investigation on Particle-gas Flows in Open Vortex-based Solar Cavity Receivers
… flow behaviour within it, such as particle egress through the aperture and convective heat losses under solar radiation, means many gaps in understanding remain. Potential safety risks, environmental pollution, and thermal efficiency issues all result from particle egress through the open …
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Viral subversion of host cell membrane trafficking
… cellular machinery to facilitate envelopment and egress of virus progeny. This thesis examines egress-related interactions between host cell factors and proteins of two different enveloped viruses: hepatitis D virus (HDV) and herpes simplex virus 1 (HSV-1). HDV is a small RNA virus causing …
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Static and Microfluidic Live Imaging Studies of Plasmodium falciparum Invasion Phenotypes
… of an optimised imaging platform for recording egress-invasion sequences. I used live cell microscopy to understand this stage of malarial infection better, by monitoring egress-invasion sequences in live cultures under controlled conditions and addressing the morphology and kinetics of …
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Evasion of interferon-gamma responses by Toxoplasma gondii in murine and human fibroblasts
… pathways. Furthermore, we observed parasite egress from IFN[gamma]-stimulated cells before replication, but inhibition of egress did not prevent cell death. Thus, the inhospitable intracellular environment of dying IFN[gamma]-stimulated human fibroblasts triggers parasite egress. This …
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Recent Thymic Emigrants and their Biomarkers
… Recent Thymic Emigrants. To this extent, nearly egress-ready thymocytes, fully egress-ready thymocytes and naïve PBMCs were obtained from pediatric donors, and both bulk and single cell RNA sequencing was performed to provide insight into the gene expression in these stages of T cell development …
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Exploring the link between RAG1 Cellular Distribution and Components of the CRL4DCAF1 E3 Ligase
… in regulating its activity. RAG1 nucleolar egress and puncta formation are dependent on the same domain as DCAF1 association. We investigated whether DCAF1 or the CRL4 ligase is responsible for regulating RAG1 localization within HEK293T cells. Treating transfected cells expressing RAG1 with …
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Autonomous Aerial Localization of Radioactive Point Sources via Recursive Bayesian Estimation and Contour Analysis
… position and intensity estimates, and finally egress from the area of interest back to the launch site. In all five steps, time is of critical importance: faster responses promise potentially saved lives. A time-optimal ingress and egress path planning method solves the first and last steps. …
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