Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"Efflux pump"”.
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Cloning and characterization of AdeMNO RND efflux pump of Acinetobacter baumannii
… of Resistance-Nodulation-cell Division (RND) efflux pumps in the antibiotic resistance mechanism of A. baumannii. Expression of six different RND pumps was analyzed in clinical isolates of A. baumannii. A novel RND family pump, AdeMNO, was found to be present in a majority of isolates. The …
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Structure and Dynamics of AcrA, a Periplasmic Component of a Multidrug Efflux Pump
AcrA is the periplasmic component of an efflux system AcrA-AcrB-TolC, which can expel different classes of antibiotics. AcrB is the inner membrane (IM) pump that utilizes proton-motive force for the active transport, TolC is the outer membrane (OM) channel, and AcrA coordinates the actions of AcrB …
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Characterisation of bedaquiline resistance mediated by the <i>M. tuberculosis</i> MmpS5L5 efflux pump
… a transcriptional repressor of the MmpS5-MmpL5 efflux pump. MmpS5L5 can efflux bedaquiline and a spectrum of anti-TB drugs in clinical development, making this mode of drug resistance highly consequential. Despite its importance, little is known about the pump’s structure and substrate …
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Assay Setup for High Throughput Screening to Identify Novel Efflux Pump Inhibitors against Escherichia coli
… kuten E. colille, on ominaista efluksipumput, joiden yli-ilmentäminen soluseinärakenteessa on seuraus efluksipumppujen pumppauskapasiteetin ylittymisestä esimerkiksi bakteereiden altistuessa pitkäjatkoisesti matalille, bakteerikasvua estämättömille antibioottipitoisuuksille. Tutkituin …
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Design, synthesis and biological evaluation of verapamil analogues, reversed isoniazids and hybrid efflux pump inhibitors against Mycobacterium tuberculosis
… drug degradation, target modification and drug efflux. Drug efflux by efflux pumps (EPs) is one of the major mechanisms responsible for the development of drug tolerance leading to the emergence of drug resistance. These efflux pumps are regulated by the house keeping proteins present in the …
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Stressed Out: Resistance-nodulation-division (RND) and Major Facilitator Superfamily (MFS) Efflux Pump Mediated Survival Strategies of Acinetobacter baumannii
… dissertation is focused on the role RND and MFS efflux pumps play in A. baumannii’s survival strategies under various stress conditions. The first goal was to functionally characterize two putative EmrAB-like MFS pumps, identified as being overproduced in RND efflux deficient strains of A. …
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Testing Whether MRP4 (a cAMP Efflux Pump) and the Beta 2 Andrenergic Receptor (an Upstream Regulator of cAMP Signaling Pathways
… Aim: MRP4/ABCC4 is an ABC transporter that can efflux the second-messenger, cAMP, from cells. MRP4 has a PDZ interacting motif at its carboxy terminal end through which it binds to scaffolding proteins NHERF1 and PDZK1. Previous studies have shown that PDZK1 serves as a scaffold physically …
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Characterization of NfxB and PA4596, Two Repressors of the mexCD-oprJ Operon Encoding an RND-Type Multidrug Efflux Pump in Pseudomonas aeruginosa
MexCD-OprJ is an RND-type multidrug efflux pump present in P. aeruginosa and is capable of exporting, and as such providing resistance to, several clinically important antimicrobials including fluoroquinolones, cephems, macrolides, and several biocides including chlorhexidine (CHX). Expression of …
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Investigating antimicrobial resistance mechanisms in Neisseria gonorrhoeae using peptide probes
… of resistance is the expression of a multidrug efflux pump termed MtrCDE, which exports a wide range of antimicrobial agents. Efflux pumps are membrane-bound systems and consequently challenging to study and target with drugs. The transcriptional regulator (MtrR) of the efflux pump, however, is …
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On the Structure and Function of Multidrug Efflux Pumps
… export drugs through the intrinsic activity of efflux pumps. In this work, a summary is provided of our current understanding of the structures and molecular mechanisms of multidrug efflux pumps in bacteria (Chapter 1). The emerging picture of the structure, function and regulation of efflux …
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Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus
… genes, and overexpression of the efflux pump-encoding gene abcC, among a large collection of highly triazole- resistant clinical A. fumigatus isolates. Upon revealing that these mechanisms alone cannot substantiate the majority of triazole resistance exhibited by this collection, …
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The Role of Multidrug Efflux Pumps in the Stress Response of Pseudomonas aeruginosa to Organic Contamination
… damage due to the upregulation of multidrug efflux pumps. Further study of this PCP-mediated induction of efflux pumps revealed a simultaneous increase in antibiotic resistance. It was discovered that the resistance nodulation-cell division (RND) efflux pump, MexAB-OprM, in particular is …
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Sweet relief: regulating the response to glucose phosphate stress by the small RNA SgrS & Co.
… response. SetA is a proton motive force-driven efflux pump capable of transporting various sugars and sugar analogs in vitro. The second part of my graduate study shows that setA expression is induced in response to glucose-phosphate stress, and this requires SgrR. Under stress conditions, setA …
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… growth. We also observed that the use of efflux pump inhibitors in combination with the fragments enhanced their antibacterial properties and may help combat multidrug resistance. In addition, using a chemoenzymatic isotopic labeling strategy, we observed by NMR a slight modification of …
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Genetic interaction landscape of the Escherichia coli transcriptional factor machinery
… of cell division, putrescine pathway and efflux pump, and cold shock adaptation, respectively. Pan-bacterial conservation suggests that TF genes with similar GI profiles are co-conserved in bacterial evolution. Moreover, the E. coli TF GI network provided deep insights on conserved genetic …
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Efflux-Mediated Multidrug Tolerance in Replicating Mycobacteria
… residence through the induction of distinct efflux pumps that are also required for intracellular growth. My findings provide a mechanistic explanation for the clinical tuberculosis treatment data gathered over the last 50 years where the length of treatment required for cure correlates with …
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Genetic mechanisms contributing to antimicrobial resistance: gene repression in Mycobacterium, gene transfer in Salmonella, and phage sensitivity in Escherichia
… studies to investigate the regulation of efflux pump expression in Mycobacterium intracellulare, regulation mechanisms of natural competence in Salmonella Typhimurium, and phage therapy of Enterohemorrhagic Escherichia coli serogroup O157:H7. A recent study showed specific mutations in the …
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Structure & Function of Bacterial Transport Machines in their Cellular Context
… are the MacA-MacB-TolC (MacAB-TolC) antibiotic efflux pump and the Type I Secretion System (T1SS), responsible respectively for antibiotic resistance and export of protein virulence factors. To pass the Gram-negative envelope in a one-step translocation process, both machines use a tripartite …
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The Chemical Analysis and Biological Activities of the Secondary Metabolites from Dalea mollis and Dalea albiflora
… not limited to, the production of biofilms and efflux pumps. Efflux pumps prevent antimicrobial drugs from reaching their biological target, so the coordinated use of efflux pump inhibitors and antimicrobial drugs has been identified as a potential treatment for multidrug-resistant …
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