Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Early embryo development"”.
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A Genomic Perspective on Early Embryo Development in Cattle
In vitro embryo production (IVP) has revolutionized the cattle industry, but it still faces efficiency limitations compared to in vivo embryo production. With a major impact on it, a proper transcriptome profile is crucial for an embryo to undergo pre-implantation development and become …
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Role of m<sup>6</sup>A RNA "reader" YTHDF2 in Mammalian Germ Cells and Early Embryo Development
… role in regulation of gene expression in development and disease. The m6A-RNA modification mostly destabilizes mRNA levels and its role is mediated by protein that binds the m6A-RNA with high affinity, YTH-domain family protein 2 (YTHDF2). However, little is known about the role of m6A-RNA …
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Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
Human naïve embryonic stem (HNES) cells can self-renew under defined culture conditions and closely resemble the preimplantation epiblast lineage. Whilst some of the signalling cascades that are fundamentally implicated in modulating the naïve gene regulatory network have been studied, our …
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MicroRNA expression and function during porcine oocyte maturation and early embryonic development
<p>Understanding oocyte maturation and embryo development in the pig is essential to improving farm animal reproduction efficiency. Many reproductive tissues are associated with a high degree of transcriptional and translational turnover that may be in part regulated by non-coding RNA such as …
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Exploring methods to understand bovine embryo competency in vitro
The development of a preimplantation embryo is a stepwise process consisting of morphological, biochemical, and genomic changes. Much remains unknown about the attainment of embryo competency to develop and establish pregnancy. To investigate this, we compared methods of selection at the oocyte or …
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Magnetic Micromotors in Assisted Reproductive Technology
… surgery (laparoscopy) approach to achieve early embryo transfer after in vitro fertilization. In both applications, magnetically actuated micromotors are utilized to capture, transport, and deliver individual cells in a reproducible, controllable manner. In comparison with established in …
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The effect of uterine flushings on early bovine embryo development in vitro
… cows on day 6, 10, or 15 post insemination on early embryo development. Ninety-eight cows were superovulated with follicle stimulating hormone. Embryos were collected by non-surgical flushing procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in …
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Characterisation of Local Mechanical Properties in Living Tissues
… of cells and tissues, especially during early embryo development. Although a wealth of morphological data characterises the shapes and movements of cells in embryos, how these movements are driven, patterned and controlled, and how this patterning is related to the mechanical properties …
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Modulation of bovine preimplantation embryonic development and pregnancy by FGF2, LIF, and IGF1
In vitro embryo production enhances genetic selection through capitalizing on superior genetics, improving fertility, and increasing animal efficiency, all of which contribute to sustainable food animal production. Of the livestock species, cattle use more embryo technologies than any other. While …
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Transcription factor Pax6 protects mouse cortical cell fate specification
… regions of the developing nervous system. Early embryo development is dependent on the balance between cell proliferation and differentiation. The process of cortical progenitor pool expansion, division and production of postmitotic cells starts at around embryonic day (E) 10.5. PCs arise …
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Factors affecting the quality and function of the bovine periovulatory follicle
… the cumulus-oocyte complex to progress through early embryonic development, and to investigate the relative effects of estradiol and progesterone on oocyte maturation and early embryo development. Ability of GnRH to elicit an ovulatory response when administered as an epidural was evaluated in …
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Computational discovery of Cis-regulatory elements in multiple drosophila species
… patterns of gene expression in D. melanogaster embryogenesis to identify sets of developmentally co-expressed genes. We developed a computational method that identifies DNA binding sites for transcription factors from families of co-regulated genes that are expressed during Drosophila embryo …
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Xenogenous Intrafallopian Transfer of Horse (Equus caballus) Gametes
… was undertaken to evaluate fertilization and early embryo development of in vitro matured (IVM) horse oocytes following transfer with homologous sperm to the oviduct of estrous ewes. A total of 1023 follicles (5.1 per ovary) were found after processing 202 slaughterhouse ovaries by aspiration …
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Spatiotemporal control of gene expression in Caenorhabditis elegans
… the myriad of different cells generated during development. Profiling genome-wide gene expression landscapes in different tissues has improved our understanding of the physiological and pathological processes taking place during development. Yet, the mechanisms underlying cell-type specific …
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Assessment of Murine Embryo Development Following Electroporation and Microinjection of a Green Fluorescent Protein DNA Construct
… The purpose of this study was to assess in vitro development, transgene expression, and integration following pronuclear or cytoplasmic microinjection of condensed or linear green fluorescent protein DNA into murine embryos using electroporation. In experiment 1, the effect of embryo orientation …
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Dynamic changes of TDP-43 in testicular and epididymal sperm
… TDP-43’s putative role in fertilization and/or early embryo development.
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Infertility and infertility treatment: childhood cancer and epigenetic risks.
… occur during pregnancy for cancers that arise early in life. Parental infertility and infertility treatment have been hypothesized as possible risk factors for childhood cancer. The link is suspected since many infertile couples may have specific genetic or epigenetic anomalies that could be …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
Implantation is the hallmark event during early pregnancy where a connection between the embryo and mother is formed. During the following phase of early postimplantation development, the pluripotent cells of the embryonic disc, the embryo proper, undergo gastrulation. This key process establishes …
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DISSECTING THE ROLE OF REACTIVE OXYGEN SPECIES IN EARLY MAMMALIAN EMBRYOGENESIS
… induce oxidative stress (OS) in both gametes and embryos. OS arises when ROS production exceeds a critical threshold, or when intracellular antioxidant systems are unable to neutralize them effectively. OS is a key factor impairing embryo quality during the processes of oocyte maturation, …
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Loss of basigin expression in the uterus reduces fertility in female mice
… angiogenesis. In mice, global ablation of Bsg is embryonic lethal, which complicates the studies on the role of BSG in reproduction. To overcome the embryonic lethality, we generated a progesterone receptor (PR)-Cre Bsg conditional knockout (cKO) mouse model and used this animal model to test this …