Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 75 for “"Early embryo"”.
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Investigations of steroidogenic capabilities of the early embryo
ICR albino mouse embryos (n=4lS0) were used to determine production of progesterone and estradiol. In Experiment I, cultures containing 20 (n=lO), 40 (n=lO) or 60 (n=6) early blastocysts were incubated in 13 X 100 rom tubes with .25 ml BMOC-2 for 20 h under 5% CO2 and air at 37C. Also, 20 (n=4) , …
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A Genomic Perspective on Early Embryo Development in Cattle
In vitro embryo production (IVP) has revolutionized the cattle industry, but it still faces efficiency limitations compared to in vivo embryo production. With a major impact on it, a proper transcriptome profile is crucial for an embryo to undergo pre-implantation development and become …
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The Role of GDF-3 in Patterning the Early Embryo
<p>The central aim of modern embryology is the resolution of the signaling pathways and transcriptional networks that direct embryonic development. After a century of rich experimental embryology, more recent molecular analyses of embryogenesis have revealed that communication between cells drives …
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Protein phosphorylation and cell diversification in the mouse early embryo.
… of compaction of the mouse preimplantation embryo. Compaction is a post-translationally controlled rearrangement of cell contacts and the cytoskeleton that occurs at the 8-cell stage of development. This re-arrangement seems to be necessary for the differentiation of the two cell types …
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Effects of Lipopolysaccharide Induced Inflammatory Response on Early Embryo Survival in Ewes
Early pregnant ewes, 5 or 6 day post coitus (dpc) were used as a model to study early embryonic loss via gram-negative bacterial infections, such as mastitis. Ewes 5/6 dpc were injected with a gram-negative bacteria cell wall component, lipopolysaccharide (LPS) or tumor necrosis factor-alpha …
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Role of m<sup>6</sup>A RNA "reader" YTHDF2 in Mammalian Germ Cells and Early Embryo Development
… generation of competent oocytes that can support early embryo development. Our molecular and bioinformatics analysis show that YTHDF2 is important for the fine tuning of the maternal transcriptome through destabilization and that its function is dependent on m6A-RNA modification.
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Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
Human naïve embryonic stem (HNES) cells can self-renew under defined culture conditions and closely resemble the preimplantation epiblast lineage. Whilst some of the signalling cascades that are fundamentally implicated in modulating the naïve gene regulatory network have been studied, our …
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The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
… regulatory mechanisms are employed during the early Drosophila embryo and it has been established as a system well suited for the study of these mechanisms. In Drosophila, ORB2 is an RBP expressed in the early embryo. It has been shown to regulate translation of bound mRNAs, but relatively …
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Magnetic Micromotors in Assisted Reproductive Technology
… surgery (laparoscopy) approach to achieve early embryo transfer after in vitro fertilization. In both applications, magnetically actuated micromotors are utilized to capture, transport, and deliver individual cells in a reproducible, controllable manner. In comparison with established in …
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Investigating processing body condensation, material properties and function during early Drosophila development
… before they are translated and degraded in the early embryo. How P bodies differentially regulate maternal RNAs is not fully understood. Using a combination of in vivo and in vitro assays, I show that P bodies in the mature oocyte exist as multilayered viscoelastic condensates that are regulated …
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Factors affecting the quality and function of the bovine periovulatory follicle
… the cumulus-oocyte complex to progress through early embryonic development, and to investigate the relative effects of estradiol and progesterone on oocyte maturation and early embryo development. Ability of GnRH to elicit an ovulatory response when administered as an epidural was evaluated in …
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Morpho : a self-deformable modular robot inspired by cellular structure
… Such deformations play a role in many processes: early embryo shape change, heart and intestine function, and in lamprey locomotion. Modular robotic systems that replicate the basic deformable multicellular structure have the potential to quickly generate large-scale shape change and create …
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DNA Methylation Landscape of the Fibrinogen Gene Cluster in the Equine Embryo
… of equine pregnancy includes the unique phase of embryo mobility from day 9 until fixation on day 15. Fixation of the embryo to the uterine wall occurs before a microvillous attachment at ~day 40. Throughout the mobility period the equine embryo expresses and secretes fibrinogen, a protein best …
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Isolation, characterization, and transcriptional autonomy of theout at first gene of Drosophila melanogaster
… kb RNAs) uniformly distributed throughout the early embryo, and a zygotic component (primarily the 3.3 kb RNA) expressed in small clusters of cells in the segments of germ band extended embryos and in the brain and nerve cord of older embryos. oaf transcripts are also present in the developing …
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MicroRNA expression and function during porcine oocyte maturation and early embryonic development
<p>Understanding oocyte maturation and embryo development in the pig is essential to improving farm animal reproduction efficiency. Many reproductive tissues are associated with a high degree of transcriptional and translational turnover that may be in part regulated by non-coding RNA such as …
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Mechanisms of toxicity in cell models of FSHD
… Whereas DUX4 expression normally occurs during early embryonic development, ectopic DUX4 expression is highly toxic in human cells. In my thesis work, using a new cell model with regulated expression of DUX4, I found that expression of DUX4 leads to the stabilization of normally rapidly …
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The Biological Effects of Novel Synthetic Retinoids
… a role in many developmental processes in the early embryo as well as being a potent tool for in vitro differentiation of stem cells. The exogenous application of these naturally occurring retinoids as well as some of their synthetic analogs has been shown to yield digit duplication phenotypes …
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A meiosis-specific form of the Anaphase Promoting Complex/Cyclosome regulates the oocyte-to-embryo transition in Drosophila
… genetically, and overexpression of Mtrm in the early embryo causes developmental defects in a subset of embryos. This work contributes to our understanding of the meiotic cell cycle and the specific regulation that distinguishes it from mitosis.
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Gene expression and imprinting in mice cloned by nuclear transfer
… to those seen in cloning are found upon in vitro embryo culturing, a process known to affect the expression of some imprinted genes. Because imprinting is normally established in the gametes and maintained in the early embryo, imprinted genes are likely to be resistant to reprogramming after …
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Exploring methods to understand bovine embryo competency in vitro
The development of a preimplantation embryo is a stepwise process consisting of morphological, biochemical, and genomic changes. Much remains unknown about the attainment of embryo competency to develop and establish pregnancy. To investigate this, we compared methods of selection at the oocyte or …
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