Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"EXTRACELLULAR PROTEASES"”.
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Erwinia carotovora extracellular proteases: characterization and role in soft rot
… of this research were to (1) characterize Ecc extracellular protease (Prt) and (2) elucidate its role in potato soft rot. A gene encoding a Prt, <u>prt</u>1, was cloned from cosmid pCA7 containing Ecc genomic DNA into plasmid pSK1. <u>Escherichia coli</u> transformed with pSK1 or pSK23, a …
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Proteases in Staphylococcal Arthritis
Proteases in Staphylococcal Arthritis Ann-Marie Calander Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, Göteborg University, Guldhedsgatan 10A, 413 46 Göteborg, Sweden Staphylococcus aureus (S. aureus) is a ubiquitous bacterium. Due to an increasing prevalence of …
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Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
Extracellular proteases and lipases from strains P26, 32A, Pl, and P27 of Pseudomonas fluorescens were produced using a dialysis membrane method. The proteases were partially purified by molecular exclusion chromatography (MWC0=60,000) and selected biochemical characteristics were determined. …
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Nanotechnology approaches for improvoved vasoactive intestinal peptide based-drug delivery systems
… VIP impairs the hydrolytic activity of extracellular proteases, leading to VIP-mediated functional responses after harsh conditions resembling the extracellular circulating proteases milieu. We used several experimental approaches combining physical and chemical characterization to …
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Investigation of quorum sensing process in Bacillus licheniformis
… termed as quorum sensing molecules, in the extracellular milieu until a threshold concentration triggers alteration in the expression of specific genes culminating in variety of responses including virulence, bioluminescence, sporulation, biofilm formation and secondary metabolites …
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Synthesis and biological evaluation of structurally diverse indole-based analogues as inhibitors of tubulin polymerization and synthesis of drug-linker constructs cleavable by enzymes present in the tumor microenvironment.
… from BDCs through cleavage of linkers by extracellular proteases, such as urokinase-type plasminogen activator (uPA) or plasmin, that are upregulated or activated in the tumor microenvironment. We have incorporated plasmin-targeted (D-Ile-L-Phe-L-Lys) and uPA-targeted (Gly-Gly-L-Arg) short …
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Polymerizable Peptide Monomers for the Targeted and Intracellular Delivery of Cancer Therapeutics
… half-lives, are susceptible to degradation by extracellular proteases, and are unable to cross cell membranes and access intracellular targets. An antibody-targeted, pH-responsive polymeric system was recently developed and implemented for the intracellular delivery of the pro-apoptotic BH3 …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… the isolation of extreme thermophiles producing extracellular proteases, and the biochemical characterisation of a stable, chelator-insensitive protease. Two plate assay systems were developed for the initial screening of proteases. The first involved the incorporation of various protease …
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Defining genomic drivers of evolution in the entomopathogenic Serratia spp. A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… pathotypes. Differences were exclusive to extracellular nuclease production (in non-pathogenic isolates and S. entomophila vs S. proteamaculans) and siderophore production in hypervirulent isolates. While the plasmid disease-encoding genes are well characterised, the mechanism driving …
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Quasivirus Infection Model Identifies a Novel Role for Protein Arginine Methyltransferase 1 in the HPV Lifecycle
… viral entry requires heparin sulfate moieties, extracellular proteases, and actively replicating host cells. Cocultures provide a large population of actively replicating primary keratinocytes and the growth inactivated fibroblasts provide the ECM components necessary for viral entry. In …
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ΜΕΛΕΤΗ ΤΗΣ ΠΡΩΤΕΑΣΗΣ ΚΑΙ ΛΙΠΑΣΗΣ ΨΥΧΡΟΤΡΟΦΟΥ ΒΑΚΤΗΡΙΑΚΟΥ ΣΤΕΛΕΧΟΥΣ ΤΟΥ ΝΩΠΟΥ ΓΑΛΑΚΤΟΣ ΚΑΙ ΕΠΙΔΡΑΣΗ ΤΟΥΣ ΣΤΗΝ ΠΟΙΟΤΗΤΑ ΤΟΥ ΤΥΡΙΟΥ ΦΕΤΑ
… OF CULTURE CONDITIONS ON THE PRODUCTION OF THE EXTRACELLULAR PROTEASE AND LIPASE FROM THE BACTERIUM WAS STUDIED. THE ENZYMES WERE PURIFIED AND THEIR PROPERTIES WERE STUDIED. THE ACTION OF PROTEASE ON THE SIMULTANEOUSLY PRODUCED LIPASE WAS EXAMINED. THE BEHAVIOR OF THE TWO ENZYME DURING THERMAL …