Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 102 for “"ES cells"”.
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
… for fast assay of relative cellular activities for homologous recombination versus random integration. In this system, a cell that has undergone a homologous recombination has red cytoplasm, while cell with a nonhomologous recombination possesses a green nucleus. The roles of important …
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The Role of Dax-1 in Regulating Pluripotency in mouse Embryonic Stem Cells
<p>The orphan receptor Dax-1 is highly expressed in pluripotent embryonic stem (ES) cells and shows a correlative reduction in expression as these cells differentiate. While it is known that Dax-1 is expressed in pluripotent mouse ES cells, the precise function of Dax-1 in these cells is not as …
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Extracellular Signalling and Stem Cell Self-Renewal
… in vitro allows for stem cell populations to be established from these lineages. Fibroblast growth factor (FGF) signalling triggers the differentiation of primitive endoderm (PrE) cells fated to contribute to the yolk sac, while cells unreceptive to FGF form the epiblast (Epi) that subsequently …
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Studying the direct effects of forces on embryonic stem cell behaviour
Cells experience different mechanical cues from their local environment, including shear flow, forces applied by neighbouring cells, and substrate stiffness. These external signals influence cell behaviour, also in embryonic stem (ES) cells, where they could potentially affect pluripotency or …
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H4K16 acetylation during embryonic stem cell differentiation
… octamer core. The N-terminal tails of the histones protrude through the DNA and can be modified by a variety of enzymes. Acetylation of Histone 4 Lysine 16 (H4K16ac) is an important modification associated with an increase in transcription, and in flies is an important component of the doseage …
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Expression Analysis and Stem Cell Engineering
The overall goal of the thesis was to develop tools to advance Investigations of stem cells in tissue engineering therapeutics for neurodegenerative disease and spinal cord injury. Two tools to characterize cell fate and a tool to separate a subset of neural cells were developed and evaluated. In …
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Genome-Wide Localization And Novel Deposition Pathways Of Histone Variant H3.3 In Embryonic Stem And Neuronal Precursor Cells
… Variant histone proteins play critical roles in the epigenetic regulation of gene expression and in the development of multicellular organisms. In this thesis, I describe the first genome-wide profiles of histone H3 variants in pluripotent mammalian embryonic stem (ES) cells, and I …
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Scalable production of cellular aggregates for the differentiation of embryonic stem cells into cardiac muscle
Embryonic stem (ES) cells have the potential to treat many diseases, such as heart disease, diabetes, and Parkinson's disease. However, large numbers of desired differentiated or progenitor cells must be generated from ES cells for many regenerative medicine applications to be successful. Current …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
The two defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued …
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Defining the Transcriptional and Epigenetic Signature of Mouse Embryonic Stem Cells with Compromised Developmental Potency
Mouse embryonic stem (ES) cells have played a crucial role in studying developmental processes and gene function in vivo. They are extremely useful in the generation of transgenic animals as they can be genetically manipulated and subsequently microinjected into blastocyst stage embryos, where they …
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Development of embryonic stem cells expressing endogenous levels of a fluorescent protein fused to the telomere binding protein TRF1
Telomeres are the repetitive DNA sequence and associated proteins found at the ends of linear chromosomes. They have a role in biological processes including meiosis and aging as well as implications in a number of genomic instability disorders and cancers. Telomeres maintain genomic stability by …
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Mapping the core regulatory circuitry of embryonic stem cells
Embryonic stem (ES) cells are of tremendous biological interest because they have the capacity, termed pluripotency, to generate any cell type of the adult organism. Our lab is interested in understanding the genetic circuitry that governs pluripotency. For my thesis work I have contributed to a …
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Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
Although embryonic stem (ES) cells and their pluripotent capability have been elucidated for decades, little study has been done on obtaining the pluripotency profile of ES cells in the incipient stages of differentiation. In this research, an ES cell line with transfected green fluorescent protein …
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Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells
… associated with changing levels of gene expression, but their role in determining, or possibly predicting, patterns of expression is uncertain. Here, the link between histone modifications and Hoxb gene expression in mouse embryonic stem (ES) cells was explored. Levels of the “active” …
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HECAT5 regulates cell proliferation via interaction with M3R in cell type-specific manner
Embryonic stem (ES) cells are derived from pluripotent inner cell mass of the early mammalian embryo. Their self-renewal and pluripotency make them attractive sources for stem cell therapies. For ethical and practical reasons, ES cells have a major threat limiting in potential therapeutic …
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Roles for chromatin regulators during differentiation of embryonic stem cells
Mammalian development involves the process by which a single fertilized egg develops into an adult with over 200 specialized cell types, each with a distinct gene expression pattern controlling its cellular state. As cells differentiate into specialized cell types, changes in the gene expression …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
During gastrulation, epiblast cells undergo an epithelial-to-mesenchymal transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic …
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Development of a High-Throughput 3D Tumor Model for Bone Sarcomas
Preclinical drug testing commonly relies on the use of two-dimensional (2D) cultures, which allow for rapid drug screening in vitro. However, 2D cultures are unable to capture the complexity of the native three-dimensional (3D) tumor microenvironment, resulting in a lack of correspondence between …
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Induced pluripotency and reprogramming by defined factors
The process by which the totipotent zygote undergoes development into an adult organism using a single genome is the foundation for epigenetics and cellular differentiation. Somatic cell nuclear transfer experiments (SCNT) provided unequivocal proof of nuclear equivalency between adult somatic …
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