Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"ERK2"”.
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Mechanism and Regulation of ERK2 Subcellular Localization
… cell system indicate that nuclear import of ERK2 is not regulated by soluble transport factors, but requires access to nucleoporins. While this process is not influenced by classical import machinery, it can be modulated by anchoring proteins that bind to ERK2 and sequester the kinase in the …
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Diverse roles for the mitogen-activated protein kinase ERK2 revealed by high-throughput target identification
… mitogenactivated protein kinases (MAPK) ERK1 and ERK2 (ERK). These kinases are critical in normal physiological processes from development to memory, and their activation in cancer drives growth and metastatic invasion. Understanding the effects of ERK signaling is especially important since …
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Search for Insulin Secretion Modulators, Novel Functions of WNK Signaling and Intramolecular Signal Transduction in ERK2
… breast cancer, and other processes, as well as ERK2 intradomain communication are discussed. Each chapter is dedicated to a separate project, but the common denominators for these projects are protein kinases involved in the WNK or ERK pathway. Through an assay developed in the lab to detect …
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Erk1 and Erk2 in hematopoiesis, mast cell function, and the management of Nf1-associated leukemia and tumors
… increased latency and potency of active Erk1 and Erk2, the principal cytosolic-to-nuclear effectors of canonical Ras-Raf-Mek signaling. Thus, Erk represents a potential regulator of leukemogenesis and tumor-associated inflammation. However, single and combined Erk1 and Erk2 roles in HSC function, …
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Nucleocytoplasmic Localization of MAPKs
… active and nuclear form of the MAPK ERK2 is sufficient for transformation of immortalized fibroblasts (Robinson MJ et al,1998). However the mechanisms of nuclear localization of MAPKs are still not fully understood clearly. Although most nucleocytoplasmic localization events require …
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Substrate Interaction and Sub-Cellular Localization in Map Kinase Pathways
… the extracellular signal-regulated kinases, ERK2 and ERK5 are two closely related enzymes that have overlapping functions in a number of cellular pathways. Sub-cellular localization and specificity towards substrates are two mechanisms of controlling the function of an enzyme in the cell. My …
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Nuclear Behaviors of ERK1/2 Signaling
… pathway functions are carried out by ERK1 and ERK2 (ERK1/2), serine/threonine protein kinases that interact with a large number of substrates in several cell compartments. ERK1/2 are crucial for major cellular phenomena such as proliferation, differentiation, and programmed cell death. Despite …
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Theoretical Studies of Protein-Protein Interactions
… called Extracellular Signal Regulated Kinase 2 (ERK2), which regulates cell cell growth, adhesion, survival and differentiation, and motor protein kinesin-1. First part of this thesis is devoted to a study of ERK2-EtsΔ138 system using variety of techniques from theoretical methods, e.g. First …
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The Regulation of Cellular Localization of Both Active and Inactive ERK1/2
… control of ERK1/2 I examined nuclear import of ERK2 mutants, the interactions of ERK2 with karyopherins β1 and β2, and explored the roles of cytoskeletal elements, motor proteins and scaffolding complexes in the localization of ERK1/2. Mutation or dysregulation of the ERK1/2 signaling cascade …
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Isoform-Specific Roles of Extracellular Signal-Regulated Kinases in Pain
… signal-regulated kinase: ERK) isoforms, ERK1 and ERK2, are believed to be key signaling molecules in nociception and nociceptive sensitization. Studies utilizing inhibitors targeting the shared ERK1/2 upstream activator, mitogen-activated protein kinase kinase: MEK), and transgenic mice expressing …
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Role of Bcl-xL in Doxorubicin-Induced Cell Death: Apoptosis and Mitotic Cell Death
… not affected by Bcl-xL overexpression. However, ERK2 was rapidly activated and its high activity was sustained in Bcl-xL-overexpressing cells treated with doxorubicin, whereas its activity was progressively decreased in doxorubicin-treated control cells. CREB, p90RSK, and NF-B, possible target …
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SCRIBBLE: A POTENTIAL DUAL KINASE INHIBITOR
… immunoprecipitation (IP) assays and discovered ERK2 as another binding partner. Surprisingly, no interaction was observed with the highly homologous MAP kinase, ERK1. No other representative kinases showed binding capabilities with Scribble. IP data confirmed that both ERK2 and ERK6 bind to …
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Regulation der Differenzierung von Ratten-Calvaria-Osteoblasten unter Einfluss von Wachstumsfaktoren
… Phosphorylierung und Aktivierung von Erk1 und Erk2, sowie von Smad1 und Smad2 mit Hilfe eines Kinase-Aktivitätsassays sowie der Westernblot-Analyse untersucht werden. Im Rahmen dieser Arbeit soll weiterhin untersucht werden, welche Bedeutung die Wachstumsfaktoren TGF-b1 und BMP-4 auf die …
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Regulation and Dysregulation via Docking Interactions in WNK and ERK1/2 MAPK Signaling
… The terminal kinases of the cascade, ERK1 and ERK2, phosphorylate hundreds of cytosolic and nuclear substrates, and in many cases protein-peptide docking interactions between ERK1/2 and substrates are required. One of the docking sites on ERK2 has been shown to be the site of a mutation, E322K, …
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Paxillin is a Novel Regulator of Xenopus Oocyte Maturation
… Raf) activates MEK1, which in turn activates ERK2. ERK2 then acts back on MOS to enhance its expression resulting in amplification of the kinase signaling cascade. To date, little is known regarding other factors that regulate this powerful feedback kinase cascade. Here we present the …
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Exploration of Chemical and Biochemical Mechanisms of Catalysis
… control cellular signaling. One such enzyme is ERK2 which plays a role in the transmission of signals by catalyzing the phosphorylation of its substrates. The signaling pathway that it is a part of is important in the control of growth, cell survival and differentiation. As with other proteins, …
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Exploration of Chemical and Biochemical Mechanisms of Catalysis
… control cellular signaling. One such enzyme is ERK2 which plays a role in the transmission of signals by catalyzing the phosphorylation of its substrates. The signaling pathway that it is a part of is important in the control of growth, cell survival and differentiation. As with other proteins, …
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Regulation of estrogen receptor-alpha mediated gene expression and endocrine resistance through estrogen receptor-alpha phosphorylation and micro-RNA in breast cancer
… ER and extracellular signal-regulated kinase 2 (ERK2) in order to understand their physiological impact on breast cancer. We identified nine miRNA- encoding genes harboring overlapping ER and ERK2 binding sites close to their transcription start sites, which require ER and ERK2 for …
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Regulation of ERK1/2 and SAPK/JNK Phosphorylation by Histamine
… concentration (10<sup>-4</sup> M) increased ERK2 phosphorylation. There was, however no significant effect seen at other concentrations (10<sup>-6</sup> M - 10<sup>-11</sup> M). Surprisingly, H1 receptor agonist betahistine (10<sup>-5</sup> M), H2 agonist amthamine (10<sup>- 5</sup> M), H3 …
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Chrysophanol-8-O-glucoside Attenuates Platelet Activation Through Inhibiting MAPK and PKCε
… of CP-8-O-glc on MAPKs, Akt, PKCε activation. ERK2, JNK1, Akt, and PKCε were significantly activated during collagen-induced aggregation, and the observation was inhibited by ERK inhibitor and CP-8-O-glc, which subsequently contributes to antiplatelet mechanism of CP-8-O-glc. On the other hand, …
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