Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"ERK1/2 pathway"”.
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REGULATION OF ERK SIGNAL TRANSDUCTION BY SIGNAL-INDUCED CYSTEINE OXIDATION
The ERK1/2 pathway plays roles across eukaryotic biology by transducing
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Exploiting tumour cell dependency on pro-survival BCL2 proteins with BH3 mimetics
The RAS-RAF-MEK1/2-ERK1/2 signalling pathway is one of the most frequently deregulated pathways in human cancer, with mutations in KRAS and BRAF commonly occurring in cancers of the pancreas, skin, and colon, among others. Indeed, BRAF and MEK1/2 inhibitors are approved for the treatment of …
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Development and application of a high throughput cell based assay to identify novel modulators of ERK1/2 activation and,Functional characterisation of the candidate Radial spokehead like (Rshl1)
… involved in the modulation of cell signalling pathways, specifically the activation of the ERK1/2 pathway, 2) to apply this assay in a screen of previously uncharacterised proteins, and 3) to characterise one candidate protein from this assay and to validate its association with the ERK1/2 …
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Protein Kinase C as an Apoptosis Regulator and a Potential Prognostic Factor in Breast Cancer
… cell lines with constitutive activation of the ERK1/2 pathway due to activation mutations of Ras and Raf. The apoptosis induced by PKCδ silencing was found to be accompanied by further increased MEK1/2 and ERK1/2 phosphorylation as well as reduced levels of the ERK1/2 phosphatase MKP3. These …
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Mitochondrial Instability, Lack of Erk1/2 Activation and High Expression of P75NTR in Engrailed-Deficient Mesencephalic Dopaminergic Neurons
… study was to elaborate more on the molecular pathway of death and to find out the mechanisms of survival by interfering with this process. In this work, I demonstrate that reduction in expression of Engrailed in mesDA neurons results in a higher sensitivity to mitochondrial apoptotic insult. …
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Regulation of C-RAF stability by the RanBPM/CTLH complex
… identified RanBPM as an inhibitor of the ERK1/2 pathway through the modulation of C-RAF protein levels. This study shows that RanBPM-mediated degradation of C-RAF occurs through the proteasome and the entire CRA domain of RanBPM is necessary for direct interaction with C-RAF and for …
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Long-Term Hypoxia Alters Ovine Fetal Adrenal eNOS and Cortisol Biosynthesis
… via phosphorylation through key signaling pathways. In examining the effects of the MEK/ERK1/2, PI3K/Akt, and calcium signaling pathways, we found that the MEK/ERK1/2 pathway and calcium do not regulate eNOS phosphorylation (peNOS), but the PI3K/Akt pathway, along with ACTH, regulates peNOS …
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Biomarker Accessible and Chemically Addressable Mechanistic Subtypes of BRAF Melanoma
… Nevertheless, pathological activation of the ERK1/2 regulatory system is a linchpin tumorigenic mechanism associated with the majority of both primary and recurrent disease. To avoid common resistance mechanisms associated with perdurance of ERK1/2 signaling, we sought to identify therapeutic …
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Activation of the Transient Receptor Potential Vanilloid-1 (TRPV1) channel mediates Extracellular Signal Regulated Kinase (ERK) phosphorylation via Beta-arrestin-2 signaling
… also acts as a signaling scaffold for the MAPK (ERK1/2) pathway which was described as an important nociceptive marker. In this thesis, several experimental approaches were employed to investigate TRPV1 signaling and to characterize whether β-arrestin-2 as well as ERK play a role downstream of …
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A study of the regulation of oestrogen receptor alpha by tissue factor in breast cancer cells
… β1 integrin leading to the activation of the ERK1/2 pathway, but was independent of PAR1 and PAR2 activation. Furthermore, exogenous TF suppressed oestradiol-mediated cell proliferation through a pathway partly mediated through PAR2 activation, and also reversed the inhibitory influence of …
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Functional assessment of BRAF and CRAF through Base Editor 3 mutagenesis reveals residues and regions critical for ERK1/2 signalling
The RAS-regulated RAF-MEK-ERK1/2 signalling pathway is critical for transducing extracellular signals from receptor tyrosine kinases at the plasma membrane through to protein targets to direct cell fate. Components of this pathway are frequently mutated to drive enhanced signalling in cancer and a …
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Investigation of mTOR-independent regulation of macroautophagy
… explores the role of CMGC kinase family members ERK1/2 and CCNB1-CDK1 in the regulation of autophagy. The ERK1/2 signalling cascade is activated in a high proportion of cancers. ERK2 has been proposed as a regulatory kinase of TFEB; however, we found little evidence to suggest that ERK1/2 was a …
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The Effect of L-DOPA and Natural Products on Age-Related Motor Deficits and Dopaminergic Cell Survival
… cell death and the expression and activation of ERK1/2 and ERK5, kinases known to protect from oxidative stress, were examined in DA cells. Resveratrol and pinostilbene decreased DA-induced cell death and activated ERK1/2, but not ERK5. Pretreatment with U0126, an inhibitor of the ERK1/2 …
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Estudo do papel do lpg2 na migração de células dendríticas infectadas por Leishmania infantum
… Finally, to investigate the activation of the ERK1/2 signaling pathway, we evaluated the expression of ERK1/2 protein using the western blot technique. RESULTS: The results demonstrate a reduction in dendritic cell migration following infection with L. infantum Δlpg2 compared to cells infected …
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Determining the Effects and Signaling Properties of β-Blockers in House Dust Mite-Driven Murine Models of Asthma
… inhibition of the β2AR- associated β-arrestin-2-ERK1/2 pathway. All our previous studies were conducted in ovalbumin-driven murine models of asthma. However, ovalbumin models have been criticized for lacking clinical relevance. To address the limitations of the ovalbumin models, we tested the …
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PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… RAF-MEK-ERK cascade, alongside additional pathways that promote cellular proliferation, survival and migration. KRAS traffics to the plasma membrane in order to be activated and bind to its effector proteins. Despite almost 40 years of research, there has been limited success in direct …
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ERK1/2 signalling and protein ubiquitylation in the control of apoptosis
… are tightly regulated by cell fate signalling pathways. The ERK1/2 signalling pathway is a key regulator of cell intrinsic apoptosis, in part through regulation of the pro-apoptotic protein ‘BCL2-interacting mediator of cell death’ (BIM). BIM is phosphorylated by ERK1/2 and this serves to drive …
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MAPK and pro-inflammatory mediators in the walls of brain blood vessels following cerebral ischemia
… and mitogen activated protein kinase (MAPK) pathway because microarray work [8] and published data [9] primarily pointed at these. These parameters and the relationships between them were studied in the cerebrovascular walls after ischemic and hemorrhagic strokes. This study lends further …