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Showing 1 to 10 of 10 for “"ERG11"”.
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ERG11-Mediated Azole Resistance in Candida albicans
… to efflux transport, point mutations in the <em>ERG11</em> gene, whose gene product is the target of azoles, result in reduced target binding affinity without precluding enzymatic function. In addition to point mutations, overexpression of <em>ERG11</em> has also been shown to decrease …
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An Investigation into Clinically Relevant Determinants of Azole Resistance in Candida albicans
… and MDR1 as well as increased expression of ERG11, encoding the azole target (14α-lanosterol demethylase, also known as CYP51) are all primary mechanisms of azole resistance that arise in azole-resistant clinical isolates. These changes are known to be mediated through gain-of-function …
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Expression Pattern of Drug-Resistance Genes in Matched Clinical Isolates of Candida albicans at Variable Fluconazole Exposure Times
… azole targeting lanosterol 14α-demethylase (Erg11) in the ergosterol biosynthesis pathway. In some clinical isolates, resistance to FLC is due to overexpression of a) the encoding genes CDR1 and CDR2 for the ABC Transporters, b) the Major Facilitator Transporter encoding gene MDR1, or c) …
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Delineating the Upc2A Regulon in Candida glabrata
… a Upc2A-dependent way, including ERG2, ERG3, and ERG11, the promoters of 10 of which were bound by Upc2A. A total of 15 genes were upregulated, including ERG2, ERG3, ERG25, and ERG11 in the strain containing GOF mutation and the promoters of 6 of these genes were bound by Upc2A. Based on our data, …
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Transcriptional Regulation of Azole Antifungal Resistance in Candida albicans
… encoding efflux pumps and the up-regulation of ERG11, a gene encoding the azole drug target lanosterol demethylase. The overexpression of efflux pumps remain the major contributor of azole resistance. The ATP binding cassette (ABC) transporter genes CDR1 and CDR2 have been shown to be regulated …
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Phenotypic and molecular antifungal susceptibility testing of yeast isolates from Pretoria
… performed on C. albicans isolates to amplify the ERG11 gene and the FKS1 gene. Sequencing was done for the amplification products and the sequence data were analysed by the CLC genome workbench software. Among the 250 isolates collected, Candida species accounted for 82.8% and C. neoformans …
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Diagnostic characterization of Malassezia pachydermatis isolates from canine otitis externa
… media. Sequencing of the miconazole drug target, ERG11, and a miconazole disk diffusion assay were used to assess susceptibility compared to the type strain and investigate substitutions to the drug target as a mechanism of reduced susceptibility. Multiple isolates had a reduced susceptibility to …
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The Molecular Mechanisms of Antifungal Drug Resistance In Pathogenic Fungi
… is overexpression and/or point mutation of ERG11, which is a rate-limiting enzyme in the ergosterol biosynthetic pathway. Other antifungals also target the ergosterol pathway, including fenpropimorph,(morpholine) which targets Erg24p, and terbinafine (allylamines), which targets Erg1p …
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Interactions of the sterol and lipid biosynthetic pathways in yeast
… (ACC1, FAS1) und der Ergosterolbiosynthese (ERG11, ERG28, ERG5, ERG2, ERG20) führt. Diese Ergebnisse unterstützen die Beobachtung, dass es im ACC1 überexprimierenden Stamm zu einer Anreicherung von Zymosterol und gesättigten Fettsäuren kommt. Die signifikant veränderte Expressionshöhe von …
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Novel Determinants That Influence Azole Susceptibility in Candida glabrata and Candida albicans
… </em>are the transcriptional regulators of <em>ERG11</em>, the gene encoding the target of azoles in the ergosterol biosynthesis pathway. Recently two homologs for these transcription factors, <em>UPC2A </em>and <em>UPC2B</em>, were identified in <em>C. glabrata</em>. One of these, …