Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"ER Retention"”.
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A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
To determine whether the N-terminal hydrophobic sequence is also responsible for retention, the N-terminal 29 amino acids of cytochrome P450 2C1, with and without an additional 29 amino acids containing an N-glycosylation site, were fused to a soluble cytoplasmic protein, Escherichia coli …
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Generation and characterization of BAP-deficient cell lines
… expressed integral membrane proteins of the ER. In B-lymphocytes, they transiently bind to the TM domains of mIgD and mIgM, both of which are BCR subunits. BAP29 and BAP31 are discussed to play a role in controlling ER export of certain TM proteins by an unknown mechanism. In particular, BAPs …
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Study of zein protein body formation in a heterologous system (<i>Xenopus laevis oocyte</i>)
… proteins accumulate in protein bodies which are derived from the vacuole. Zeins, the major corn storage proteins, however, are retained in the endoplasmic reticulum (ER) and their protein bodies are derived from the ER. There are circumstantial and preliminary data indicating that 27K zein, the …
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Characterization of Drosophila Scap: Analysis of Mutants and Evidence for a Retention Factor
… of lipid homeostasis and it is highly conserved among metazoans. SREBP is a transcription factor whose precursor is an endoplasmic reticulum (ER) transmembrane protein. In order to be activated it must travel to the Golgi apparatus via interaction with an escort protein, Scap. Scap, in turn …
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Cellular mechanisms that modulate kainate receptor trafficking and assembly
… targeting. Cytoplasmic and non-cytoplasmic determinants that modulate KAR expression at the plasma membrane have been recently characterized. The intracellular trafficking determinants are presumed to bind to chaperone proteins, but these proteins have not been identified for any KAR subunit. …
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HSP70 as a Target for Correction of Wilson Disease Causing ATP7B Mutants
Copper (Cu) is an essential micronutrient, which operates as a cofactor of enzymes regulating a wide range of critical physiological processes. However, Cu overload compromises the redox balance in cells and tissues causing serious toxicity. Removal of excess Cu from the body is driven be …
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The role of tapasin and its isoforms in antigen presentation and tumor immunity
… MHC-I molecules in the endoplasmic reticulum (ER). This thesis examines the expression, localization and function of two novel, alternatively-spliced isoforms of human tapasin that lack exon 7 (“isoform 2”) or both exons 6 and 7 (“isoform 3”). Isoform 1 contains a di-lysine ER-retention motif; …
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Structural Mechanism of Orthopoxvirus Sabotage of MHCI Antigen Presentation
<p>Immunomodulatory proteins that subvert major histocompatibility complex class I: MHCI) antigen presentation can help viruses to evade cytotoxic T-lymphocyte: CTL) detection and clearance. Cowpox virus, like other orthopoxvirus family members, is a large DNA virus, whose genomic termini encode …
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Regulation of Polarized Protein Transport to Axons, Dendrites, and Sensory Cilia in Caeborhabditis Elegans Neurons
… result of asymmetric trafficking of a large number of cellular components including ion channels, neurotransmitter receptors, synaptic vesicles, and signaling proteins. Yet, despite the significance of asymmetric protein transport in neuronal polarity, the molecules and mechanisms that direct …
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Die Regulation der Expression des humanen Oncostatin-M-Rezeptors durch Januskinasen
The oncostatin M receptor (OSMR) is part of a heterodimeric receptor complex which mediates signal transduction of the pleiotropic cytokine OSM. Like other cytokine receptors, the OSMR does not contain intrinsic kinase activity and therefore signalling events are mediated by members of the family …
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Characterization of the glycosylation and signal peptide sequences within the vaccinia virus m2 protein and influence on ERK1/2 and NF-kB activation
… Wild-type vaccinia virus (VV) strains subvert the immune response by expressing proteins that interfere with nuclear translocation of NF-κB. In contrast, infection with an attenuated strain, MVA, results in host cell NF-κB activation. Inclusion of the M2 gene of VV into the attenuated MVA …
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Proteinkonjugationen durch Kupfer-abhängige Formylglycin-generierende Enzyme zur Generierung von Antikörper-Wirkstoff-Konjugaten
The formylglycine-generating enzyme (FGE) is utilized for the regioselective conjugation of proteins. In an oxygen-dependent reaction it catalyzes the formation of the non-canonical amino acid Cα-formylglycine (FGly) from the cysteine residue of a CxPxR-type core recognition se-quence. In addition …
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Untersuchung von Proteinkomponenten der ER-Golgi Recycling-Maschinerie von Hefe
In Hefe werden alle bisher untersuchten ER-Proteine, die zum Golgi gelangen, mit Hilfe von COPI-Vesikeln zum ER zurücktransportiert. Bisher ist aber noch ungeklärt, wie die unterschiedlichen Proteine in die Vesikel dirigiert werden.Durch Verwendung eines Sec22-a-Hybridproteins konnte in unserer …
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Functional Characterization of the Mammalian TRPV4 Channel: Yeast Screen Reveals Gain-of-Function Mutations
… are a class of six-transmembrane (6-TM) cation-permeable channels that mediate flux of calcium and sodium into cells, leading to depolarization as well as activation of calcium-mediated second-messenger signaling pathways. The TRP channel family is large and diverse in terms of tissue expression, …
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Biochemische und zellbiologische Charakterisierung des COP Vesikel vermittelten Proteintransports in Pflanzen
Während in Hefen und in Säugetieren die Beteiligung der "coatprotein-coated vesicles" (COP Vesikel) an dem intrazellulären Proteintransport zwischen ER und Golgi-Apparat unumstritten ist, konnte ihre Existenz in Pflanzen bis zum Beginn der vorliegenden Arbeit noch nicht nachgewiesen werden. Die …