Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"ENU"”.
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Charakterisierung und Validierung von ENU-Mausvarianten mit beeinträchtigter Fähigkeit zur Objekterkennung
… Manipulationen mittels der mutagenen Substanz ENU basiert. Hierbei wurden relevante Mausvarianten ausschließlich aufgrund ihres Phänotyps selektiert und zur Gründung einer neuen Mauslinie verpaart. Da es sich hierbei um einen hypothesenfreien Ansatz handelt, wurde für die …
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Phänotypische und genotypische Charakterisierung der ENU-induzierten Mausmutante HST001 zur Verwendung für die nephrologische Forschung
Phenotypic and genotypic characterization of the ENU-induced mutant mouse line HST001 for use in nephrological research A dominant mutation was established in the line HST001 within the Munich ENU mouse mutagenesis project. On the genetic background of the inbred strain C3H heterozygous mutant mice …
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Phänotypische und genotypische Charakterisierung der ENU-induzierten Mausmutante SMA002 als Tiermodell für Wachstumsdefizit und Hyperaktivität
… analysis of the dominant mutation in the ENU-induced mutant mouse line SMA002. The results were derived from the analysis of heterozygous mutant animals on the C3H genetic background. As the main phenotype of SMA002, an abnormal behavior combined with reduced body weight was revealed. The …
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An Integrated Software System for EEG/EMG-Based Forward Genetic Screen of Sleep/Wake Abnormalities in ENU-Mutagenized Mice
… in mice. We have initiated a dominant screen of ENU-mutagenized mice, in which a comprehensive set of sleep parameters is measured via EEG/EMG-based somnography analysis in basal light/dark periods as well as in the recovery period following forced sleep deprivation. A major obstacle for such …
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Identification and Characterisation of New Mouse Models for Hearing Loss
Mouse N-ethyl-N-Nitrosourea (ENU) mutagenesis programmes have been successfully employed for the identification of models of human disease, and allowed the discovery of novel gene associations. This thesis describes the characterization of two new ENU-induced mouse models of hearing loss, goya and …
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Identifying Tumor Vascular Permeability Heterogeneity Using Reduced Encoding Techniques
… Sprague Dawley rats with N-ethyl-N-nitrosourea (ENU) induced mammary tumors imaged with fast T1-weighted gradient echo DCE MRI following a Gd-DTPA injection, there is a window of resolutions that detects similar PS ""hot spots"" compared to those obtained from the clinical imager resolution. The …
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Novel techniques for measuring the effect of neighbouring bases on mutation and their applications
… and discriminate between N-ethyl-N-nitrosourea (ENU)-induced and spontaneous point mutations in the mouse germline. ENU is a synthetic chemical employed in mutagenesis studies, introducing novel point mutations to genomes. My classification results reveal that a combination of k-mer size and …
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Novel techniques for measuring the effect of neighbouring bases on mutation and their applications
… and discriminate between N-ethyl-N-nitrosourea (ENU)-induced and spontaneous point mutations in the mouse germline. ENU is a synthetic chemical employed in mutagenesis studies, introducing novel point mutations to genomes. My classification results reveal that a combination of k-mer size and …
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The Genetic Basis of Susceptibility to Therapy-related Leukemia in Mice
… strains were treated with N-ethyl-N-nitrosourea (ENU), a potent alkylating agent in mice. Six of these mouse strains were susceptible to alkylator-induced leukemia. SWR/J mice were the most susceptible in this relatively small screen. We expanded on that study to characterize SWR/J mice as a …
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Nephrotic Syndrome and Glomerular Basement Membrane: Genetic Defect of the Laminin α5 Chain
… of the MRC Harwell Ageing Screen, a large-scale ENU mutagenesis screen, a novel missense mutation (E884G) was identified in the gene Lama5. Homozygous mice showed a nephrotic phenotype including a severe proteinuria that preceded histological and ultrastructural changes. Further investigation …
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The Role of ECSIT in Mitochondrial Dysfunction Mediated Cardiomyopathy
… assembled complex I. As part of a large scale ENU mutagenesis screen, The Harwell Ageing Screen, a novel missense mutation (N209I) was identified in ECSIT which resulted in a hypertrophic cardiomyopathy phenotype in homozygous mutant animals. Further investigation revealed this phenotype to be …
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Untersuchungen zur Struktur und Funktion des Legumain-Gens im Modellsystem Maus
… Parameter wurde in Zusammenarbeit mit dem ENU Mutagenese Screen in München durchgeführt. Statistisch signifikante Unterschiede zwischen allen drei Genotypgruppen der Legumain Mauslinie 3HLeg130 konnten für die beiden Parameter Carnitin und Arginin im biochemisch-metabolischen Screen durch …
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Analysis of DOCK2 and SENP2 mutations on the immune system and CD8 T cell survival
… The mutations were discovered by genome-wide ENU-mutagenesis in mice by flow cytometric screening of lymphocyte subsets in splenocytes and in peripheral blood. Genetic mapping and sequencing identified the two mutations. The first was a premature stop-gain mutation in Dock2, encoding a GTP …
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Analysis of DOCK2 and SENP2 mutations on the immune system and CD8 T cell survival
… The mutations were discovered by genome-wide ENU-mutagenesis in mice by flow cytometric screening of lymphocyte subsets in splenocytes and in peripheral blood. Genetic mapping and sequencing identified the two mutations. The first was a premature stop-gain mutation in Dock2, encoding a GTP …
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Genetic Exploration of Hereditary Immune Diseases
… of dermal ECM proteins. Our hypothesis is that ENU caused single nucleotide mutations in one or more genes in the Tsk2/+ locus interval that determines the disease phenotype. From the literature, Tsk2/+ mice have a mutation on chromosome 1 between 42.5 and 52.5 megabases. We used microarray data …