NDLTD logo NDLTD Union Catalog and Global ETD Search

Global ETD Search

Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

Results

Showing 1 to 20 of 106 for “"EMSA"”.

  1. Transcriptional Regulation of Matrix MetalloProteinase-3 in Rat Brain Astrocytes.

    김, 광수2011

    … start codon are important in induction of MMP-3. EMSA-based study and site-direct mutagenesis revealed that AP-1 is important in induction of MMP-3. Since three AP-1 binding sites (we described them as proximal, middle and distal, respectively according to the distances from start codon) exist in …

    ajou Repository record

  2. Significant Association of ADORA3 Genetic Polymorphisms with Aspirin-Intolerant Urticaria in a Korean Population

    남, 은주2011

    … assay, electrophoretic mobility shift assay (EMSA), and real-time PCR for the mRNA expression in PBMC of AIU patients. RESULTS: The haplotype [T-1050C-564] frequency of ADORA3 gene showed significantly higher in AIAU group than in AICU and NC groups (p = 0.047, for AIAU vs. AICU; p = 0.005, …

    ajou Repository record

  3. Transkriptionelle Regulation des CD23a-Promotors in Zellen chronischer lymphatischer Leukämien vom B-Zelltyp

    Gross, Tim PhilippUniversität Würzburg

    … wurde mit Gel-Retardierungsexperimenten (EMSA) untersucht. Hierzu wurden CD23a-Core-Promotor-Oligonukleotide zusammen mit Kernproteinextrakten aus Stimulationsansätzen von B-CLL- und normalen B-Zellen mit IL-4, IFN-g und PMA genutzt. Die EMSA-Experimente zeigten trotz unterschiedlicher …

    wurz-thes Repository record

  4. Significant Association of IL-18 Genetic Polymorphisms with Aspirin-Intolerant Urticaria in a Korean Population

    손, 진경2011

    … assay, electrophoretic mobility shift assay (EMSA) and neutrophil chemotactic assay. RESULTS: The minor allele frequency of IL-18 at -607 A>C and haplotype 1 [C-607G-137] showed significantly higher in AIAU than in NC groups(p=0.019, for AIAU vs NC; p=0.048, for AIU vs NC). The …

    ajou Repository record

  5. Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

    Kibler, Clayton EugeneThe University of Texas Medical Branch at Galveston2021

    … domain. An electrophoretic mobility shift assay (EMSA) demonstrated that TRP47 directly binds host DNA via its tandem repeat domain. Utilizing chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) with E. chaffeensis-infected cells, TRP47 was found to bind at multiple …

    utmb Repository record

  6. Genetic Effect of ALOX15 Polymorphisms on Aspirin-Exacerbated Respiratory Disease

    송, 영신2011

    … assay, electrophoretic mobility shift assay (EMSA), and enzyme activity assay in red blood cell (RBC) lysates. RESULTS: No significant difference of the genotype frequency in ALOX15 genetic polymorphism was addressed. Peripheral blood total eosinophil count was significantly higher in AERD …

    ajou Repository record

  7. Regulation of the MexAB-OprM Multidrug Efflux System of Pseudomonas aeruginosa: Involvement of Pentachlorophenol and Plant Chemicals

    Starr, Lisa Michelle2010

    … PCP resistance. An electromobility shift assay (EMSA) showed that PCP interacts with NalC, interfering with its binding to the PA3720-armR promoter region and thereby promoting PA3720-armR expression. Nonetheless, the increase in ArmR did not drive mexAB-oprM expression suggesting that PCP …

    queens Repository record

  8. Interaction between positive- and negative- regulatory elements in the first intron controls transcription of the glutamine synthetase gene during adipocyte differentiation

    Ryou, Chongsuk2015

    … by electrophoretic mobility shift analyses (EMSA) and functional analyses of stably transfected fusion genes composed of putative regulatory elements alone or in combination. To identify putative transcription factors, EMSA included the use of wild type and mutant oligonucleotides as well as …

    wayne-thes Repository record

  9. 2D layout of bead based thioaptamer/aptamer selection platform for therapeutics and diagnostics

    Xu ZhaoThe University of Texas Medical Branch2007

    … Electrophoretic mobility shifting assay (EMSA) has been used to verify XBY6 binding, and ELISA assay has been used to verify I-kappa B binding towards human recombinant NF-kappa B protein. Preliminary study of bead in 2D gel showed applicability of bead-based selection and thus on bead …

    utmb Repository record

  10. Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling

    Delucchi, Anthony Benjamin2001

    … by electrophoretic mobility shift assay (EMSA) and proved to effectively form a DNA binding heterodimer with ARNT. In addition the functionality of C∆418 was assessed by co-precipitation showing that the ThioHis-produced C∆418 was indeed able to dimerize with C∆553 (an AHR deletion …

    u-pacific Repository record

  11. Phytochemische und pharmakologisch-biologische Untersuchungen von Arnica montana und Vernonia triflosculosa

    Kos, Olha

    … wurde im NF-kB EMSA und IL-8 ELISA in vitro sowie in vivo im Crotonöl-induzierten Mausohrödem-Test untersucht. <br>Die Aktivität war vergleichbar mit 11-alpha,13-Dihydrohelenalin-Derivaten. Wie erwartet zeigten 6-O-(2-Methylbutyryl) und 6-O-Methacryloylhelenalin …

    freiburg-diss Repository record

  12. Contribution to the elucidation of the anti-inflammatory activity of Sesquiterpene Lactones

    García-Piñeres, Alfonso J.

    … densitometric method for the quantification of EMSA analyses was also established. This allowed the determination of the concentration required to obtain a half-maximal inhibition of NF-kB (IC50). The method was used to calculate the IC50 values for 24 SLs. <br>Up to now, the anti-inflammatory …

    freiburg-diss Repository record

  13. Transkriptionelle Regulation des Homeo-Domänen-Transkriptionsfaktors Islet/Duodenum Homeobox-1 (IDX-1) in insulinproduzierenden Betazellen des endokrinen Pankreas

    Peter, AndreasUniversität Würzburg

    … Mithilfe von Luziferasereportergenassays und EMSA untersucht. Die Expression von IDX-1 Protein und mRNA wird durch milde Hyperglykämie stimuliert. Dieser Effekt ist auf eine Aktivierung des IDX-1 Promotors zurückzuführen. Die Aktivierung innerhalb des Promotors konnte auf zwei Regionen …

    wurz-thes Repository record

  14. Genetic Effect of MBL2 Polymorphisms in Patients with Diisocyanate-induced Occupational Asthma

    배, 수진2013

    … assay and electrophoretic mobility shift assay (EMSA). RESULT: Significantly higher frequency of the AA genotype at -431 A>C (P=0.016) and higher frequency of haplotype (ht) 2 [CAG] were noted in the DI-OA group compared to AEC group (P=0.044). DI-OA patients carrying the GG genotype at -225 G>C, …

    ajou Repository record

  15. The P22 Xis Protein: Regulation of Bacteriophage P22 Site -Specific Recombination

    Mattis, Aras NikodemasUniversity of Illinois at Urbana-Champaign2015

    … of Xis mutants were purified and assayed by EMSA and crosslinking studies. All purified mutants formed multimers by crosslinking, but L15F, K16E, A33P, S43F, and A55T were defective in DNA binding. Furthermore, L46F bound DNA with a weaker affinity but still formed specific complexes.

    uiuc Repository record

  16. Interaction of Spliceosomal U2 snRNP Protein p14 with Its Branch Site RNA Target

    Perea Vargas, WilliamThe Graduate School and University Center of The City University of New York2016

    … have used electrophoretic mobility shift assays (EMSA) and nuclear magnetic resonance (NMR) under non-denaturing conditions to establish the structural and or functional role of this splicing factor. Our EMSA results show that p14, which contains a RNA recognition motif (RRM), binds duplex RNA …

    cuny-grad Repository record

  17. DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus

    Wowor, Andy James BudimanBiological Sciences2022

    … from the electrophoretic mobility shift assay (EMSA) also support a striking difference between ds-DNA binding for Klenow and Klentaq. In EMSA, all ds-DNA/Klenow complexes show a time dependent shift from a slower to a faster moving complex while pt-DNA/Klenow complexes (both matched and …

    lsu-thes Repository record

  18. Characterization of Transcriptional and Post-transcriptional Regulation of lin-42/Period During Post-embryonic Development of C. elegans

    James, TracyVirginia Tech2012

    … assays (Electrophoretic Mobility Shift Assay, EMSA) (Chapter 2) followed by an RNA interference (RNAi)-based genetic screen (Chapter 3) to identify lin-42 transcriptional regulators. Using the EMSA, we identified three 50 to 100 base pair regions (binding regions, BR1-3) in the lin-42 5â …

    vt Repository record

  19. Etablierung und Anwendung der Chromatin-Immunopräzipitation für <i>in vivo</i>-Bindungsstudien der bZIP-Transkriptionsfaktoren TGA2.1 und TGA2.2 an Promotoren der Pathogenabwehr in Tabak

    Butterbrodt, Thomas Walter2012

    … das spät exprimierte Gen PR1a. DNA-Bindestudien (EMSA) zeigten, dass das as-1-Element von den bZIP-Transkriptionsfaktoren TGA2.1 und TGA2.2 erkannt wird. Um eine in vivo-Interaktion der TGA-Faktoren mit endogenen as-1-ähnlichen Promotorelementen zu untersuchen, wurde die …

    goettingen Repository record

  20. Sequenz-spezifische Interaktion des murinen präreplikativen Komplexes mit Origin-DNA

    Herrmann, ThomasUniversität Würzburg

    … (Komplex A) auch in vitro mit Hilfe von EMSA-Experimenten beobachtet werden. Die Bindungsaktivität von Komplex A an Fragment B konnte durch ATP verstärkt werden.

    wurz-thes Repository record

Page 1 of 6